Chromatin folks: don't miss GRC/GRS Genome Architecture in Cell Fate and Disease, June 15-20 2025 Ventura, CA. Registration deadline: Mar 18, 2025. grc.org/genome-architecture-…

Functional memory T cells are derived from exhausted clones and expanded by checkpoint blockade biorxiv.org/content/10.1101/… #biorxiv_immuno

What a PERFFect day to share our new preprint! With @tsion_abay #BobStickels @MerilTakizawa @ChaligneRonan and @Satpathology, we introduce PERFF-seq, a new experimental approach to studying rare cells with scRNA-seq via transcript-specific enrichment. biorxiv.org/content/10.1101/… 1/n

Happy to share our new work describing DESynR genes! The evolution of new human protein-coding genes relies on repurposing protein domains from ancestral genes, rather than de novo sequence creation. New genes evolved from old parts, and while we have methods that enable high-throughput generation and screening of base-pair level sequence evolution (mutagenesis, directed evolution, CRISPR, etc), we lack similar scalable technologies for domain-level evolution. We developed a high-throughput combinatorial molecular assembly method to generate thousands of barcoded novel genes from the domain building blocks of natural genes, termed DESynR (Domain Engineered via Synthesis and Recombination) genes.