Chromosome Dynamics Lab @ Católica Biomedical Research. Aiming to understand how mitotic chromosomes are assembled and influence mitotic fidelity
Joined October 2015
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Mar 27
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Excited to share our new preprint!
We uncover a new layer of control in how cells restart transcription after mitosis.
👉 TTF2 prevents premature rRNA transcription during mitotic exit (biorxiv.org/content/10.64898…)
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Mar 27
🧵1/
Excited to share our new preprint!
We uncover a new layer of control in how cells restart transcription after mitosis.
👉 TTF2 prevents premature rRNA transcription during mitotic exit (biorxiv.org/content/10.64898…)
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Mar 27
12/ Huge congratulations to the great team: @catarinaapedro @MicrodotLaura @peneda_catarina and Mia.
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Mar 27
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Our work links two fields that often don’t talk enough:
🧬 Mitosis
🧬Transcription regulation
and highlights how their coordination preserves cellular integrity
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Mar 27
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We further show that:
Disrupting this timing → nucleolar fragmentation in interphase
👉 Mitotic transcription timing has consequences beyond mitosis
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Mar 27
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Importantly, this challenges the idea that transcription simply resumes passively as cells exit mitosis.
Instead, reactivation is actively restrained and timed
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Mar 27
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This leads to:
• early rRNA synthesis
• premature recruitment of nucleolar components
👉Transcription turns ON too early
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Mar 27
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But here’s the unexpected part 👇
Loss of TTF2 triggers premature RNA Polymerase I activation during anaphase
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Mar 27
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As expected:
Loss of TTF2 → retention of chromatin-associated transcripts in metaphase
(mainly RNA Pol II–derived)
✔️ Consistent with its known role in transcriptional clearance
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Mar 27
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Using nascent RNA labeling polymerase-specific perturbations,
we tracked transcription dynamics across mitosis in:
• human embryonic stem cells
• HeLa cells
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Mar 27
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We focused on TTF2, a factor known to remove RNA Polymerase II transcripts at mitotic entry.
But what happens later—during transcriptional reactivation?
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Mar 27
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During mitosis, transcription is globally shut down.
As cells exit mitosis, transcription must be reactivated—precisely and in the right order.
How this timing is controlled remains poorly understood.
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Oliveira Lab retweeted
Feb 26
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Drop a line if you are interested to know more details and please share!
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Feb 16
🧵 Thread: What happens when transcription isn’t properly shut off during mitosis? Our new preprint explores just that 👇
biorxiv.org/content/10.64898…
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Feb 16
5/ This means that mitotic transcription isn’t just a passive consequence of cell cycle stage — it actively threatens genome stability when not properly regulated.
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Feb 16
4/ The result? When transcription isn’t cleared, sister chromatid resolution is impaired — a critical step needed to ensure faithful chromosome segregation in anaphase.
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Feb 16
3/ Importantly, we show that these defects arise because R-loops form when transcription persists during mitosis — and preventing transcription reverts the defects. That establishes a causal link.
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Feb 16
2/ Using TTF2 depletion to force nascent transcripts to persist on mitotic chromatin, we found that this abnormal retention leads to mitotic errors including abnormal chromosomes and bridges.
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Feb 16
1/ During mitosis, cells globally suppress transcription as chromosomes condense — a conserved hallmark of cell division. But what if this repression fails? 😮💨
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