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Johannes Walter @FrogWalterLab
Joined July 2020
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  • Following 31
  • Followers 429
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Check out latest from the Walter-Pellman collaboration, revealing how the replisome is remodeled in mitosis to allow CMG eviction, which we propose minimizes fallout from under-replication. biorxiv.org/content/10.1101/…

TTF2 promotes replisome eviction from stalled forks in mitosis

When cells enter mitosis with under-replicated DNA, sister chromosome segregation is compromised, which can lead to massive genome instability. The replisome-associated E3 ubiquitin ligase TRAIP...

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Complementary work on USP37 from the Labib and Emanuele labs biorxiv.org/content/10.1101/… biorxiv.org/content/10.1101/…

USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination

The CMG helicase (CDC45-MCM2-7-GINS) unwinds DNA as a component of eukaryotic replisomes. Replisome (dis)assembly is tightly coordinated with cell cycle progression to ensure genome stability....

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Thrilled to share our new finding that the DUB USP37 suppresses rogue ubiquitylation and disassembly of stressed replisomes by TRAIP. Fantastic collaboration between Olga Kochenova in my lab and Giusi D’Alessandro in Steve Jackson’s lab! Read all about it: biorxiv.org/content/10.1101/…

USP37 prevents premature disassembly of stressed replisomes by TRAIP

The E3 ubiquitin ligase TRAIP associates with the replisome and helps this molecular machine deal with replication stress. Thus, TRAIP promotes DNA inter-strand crosslink repair by triggering the...

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Great to see that, as we found in egg extracts, STK19 is also integral to TC-NER in human cells! biorxiv.org/content/10.1101/… biorxiv.org/content/10.1101/… biorxiv.org/content/10.1101/…

STK19 drives Transcription-Coupled Repair by stimulating repair complex stability, Pol II ubiquit...

DNA damage forms a major obstacle for gene transcription by RNA polymerase II (Pol II). Transcription-coupled nucleotide excision repair (TC-NER) efficiently eliminates transcription-blocking lesions...

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We recapitulated transcription-coupled repair in frog egg extracts and showed that STK19 couples stalled RNAPII to repair. Tour de force by @TychoMevissen (seeking PI positions, grab him if you can!), and key contribution from Lucas Farnung and team. biorxiv.org/content/10.1101/…

STK19 positions TFIIH for cell-free transcription-coupled DNA repair

In transcription-coupled repair, stalled RNA polymerase II (Pol II) is recognized by CSB and CRL4CSA, which co-operate with UVSSSA and ELOF1 to recruit TFIIH for nucleotide excision repair (TC-NER)....

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Please RT: The Walter lab at Harvard seeks computational biologists to extend our work using AlphaFold to find new protein-protein interactions. Contact johannes_walter@hms.harvard.edu biorxiv.org/content/10.1101/… predictomes.org/

Predictomes: A classifier-curated database of AlphaFold-modeled protein-protein interactions

Protein-protein interactions (PPIs) are ubiquitous in biology, yet a comprehensive structural characterization of the PPIs underlying biochemical processes is lacking. Although AlphaFold-Multimer...

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A warm thank you to Lumír Krejčí for hosting my Mendel lecture in Brno, Czech Republic, home of a vibrant scientific community, the marvelous Tugendhat Villa, and of course, Gregor Mendel. To stream Mendel lectures: youtube.com/@mendellectures4…

MENDEL LECTURES

The first series of Mendel Lectures was in 2003, when it was devoted to the 50th anniversary of the discovery of DNA. Since then, Brno has been visited by almost 100 scientists, including Nobel Prize...

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Excited to share (with my 8 followers) graduate student Ernst Schmid’s “SPOC” classifier, which can pick a good AlphaFold-Multimer prediction out of a crowd, and predictomes.org/, a database of PPIs in the genome maintenance space. biorxiv.org/content/10.1101/…

Predictomes | Home

A free interactive resource for viewing predicted AlphaFold multimer structures for protein pairs in biological pathways.

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Comprehensive model of how the replisome bypasses G4 quadruplexes. Intricate mechanism involving one accessory helicase that helps CMG bypass the G4, and a second helicase that subsequently unfolds the G4 for nascent strand extension. Beautiful work! biorxiv.org/content/10.1101/…

Multistep mechanism of DNA replication-coupled G-quadruplex resolution

G-quadruplex (or G4) structures are non-canonical DNA structures that form in guanine-rich sequences and threaten genome stability when not properly resolved. G4 unwinding occurs during S phase via...

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