Building synthetic metabolisms in bacteria for a sustainable future @DTUBiosustain · Views are our own #Pseudomonas’ 🧬 ·
Joined August 2018
- Tweets 793
- Following 349
- Followers 1,865
- Likes 976
225 Photos and videos
Nikel SEM Group retweeted
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions—led by @FavoinoGiusi & @OscarPuiggene @LabNikel @FEMSmicro #Microbiology #Reviews #SynBio #Metabolic #Engineering
academic.oup.com/femsre/adva…
11
33
2,505
Nikel SEM Group retweeted
3 Apr 2025
Ins and outs for designing bacterial metabolisms for C1-assimilation, from a multilevel perspective, now pieced together in this review from our @LabNikel! Check it out at academic.oup.com/femsre/adva…
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions—led by @FavoinoGiusi & @OscarPuiggene @LabNikel @FEMSmicro #Microbiology #Reviews #SynBio #Metabolic #Engineering
academic.oup.com/femsre/adva…
3
9
684
14 Mar 2025
New paper out! Check our new CRISPR toolkit for Cupriavidus necator!
13 Mar 2025
Streamlined and efficient genome editing in #Cupriavidus necator H16 using an optimised SIBR-Cas system—spearheaded by @dellavallesimo & @enricoorsi & led by Costas Patinios Wei Huang @TrendsinBiotech #SynBio
sciencedirect.com/science/ar…
5
12
925
4 Mar 2025
New paper out in collaboration w/ @mirbachh from @maxplanckpress and many more! Check Helena's thread for a detailed overview of the manuscript!👇
4 Mar 2025
Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in @NatureComms ! 🔥
Read below to see how we developed the sensors and what they are good for 🥳.
nature.com/articles/s41467-0…
1
5
375
Nikel SEM Group retweeted
4 Mar 2025
Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in @NatureComms ! 🔥
Read below to see how we developed the sensors and what they are good for 🥳.
nature.com/articles/s41467-0…
9
8
29
1,925
4 Mar 2025
New paper out from our group!
Computation-aided designs enable developing auxotrophic #metabolic #sensors for wide-range glyoxylate and glycolate detection—spearheaded by @enricoorsi w/a choral assembly of cool collaborators @LabNikel @NatureComms #SynBio #OpenAccess
nature.com/articles/s41467-0…
3
9
661
Nikel SEM Group retweeted
21 Feb 2025
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint led by @OscarPuiggene #SynBio #Methylotrophy #CircularEconomy @LabNikel
biorxiv.org/content/10.1101/…
8
27
1,778
Nikel SEM Group retweeted
21 Feb 2025
Super exciting work by @OscarPuiggene from @LabNikel on advancing #methanol use by Pseudomonas Putida by engineering #serinecycle variants! Many congratulations, excited to be a part of this !
21 Feb 2025
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint led by @OscarPuiggene #SynBio #Methylotrophy #CircularEconomy @LabNikel
biorxiv.org/content/10.1101/…
2
13
487
20 Feb 2025
Check our newest preprint 👇!
29 Jan 2025
#Preprint alert 🚨 Genetic dissection of cyclic di-GMP signaling in #Pseudomonas aeruginosa via diguanylate cyclase disruption―collaboration between @LabNikel & Andrea Smania's team in Argentina, led by @martino_RA & @Daniel__Volke @biorxiv_micrbio #CRISPR
biorxiv.org/content/10.1101/…
3
240
Nikel SEM Group retweeted
Engineering a phi15-based #expression system for stringent #gene expression in #Pseudomonas putida—led by @EvelineLammens, @Daniel__Volke in a nice collab between @LabNikel & @RobLavigne1's team
nature.com/articles/s42003-0…
8
32
1,590
20 Feb 2025
Congratulations @PabloINik on this great achievement!
We are thrilled to congratulate our group leader, @PabloINik, for being elected Fellow of ASM! A well-earned recognition of your outstanding contributions to our field! 🥳
14 Feb 2025
Honored to be elected as Fellow of the #American Academy of #Microbiology! This recognition reflects the incredible work of my past and present collaborators, students, and mentors. Deep gratitude to my nominators and the microbiology community for their support! @ASMicrobiology
27
669
Nikel SEM Group retweeted
17 Jan 2025
CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—our latest contribution led by @FilFederici & Francesco Luppino @LabNikel @DTUBiosustain #OpenAccess #SynBio
sciencedirect.com/science/ar…
10
61
2,764
Nikel SEM Group retweeted
18 Jan 2025
Amazing tool for multiple cycles of genetic engineering of Gram negative bacteria developed by @FilFederici and Francesco, now fully available! @LabNikel
17 Jan 2025
CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—our latest contribution led by @FilFederici & Francesco Luppino @LabNikel @DTUBiosustain #OpenAccess #SynBio
sciencedirect.com/science/ar…
1
3
244
Nikel SEM Group retweeted
Leveraging engineered #Pseudomonas putida minicells for bioconversion of organic acids into short-chain methyl ketones by @KaterinaKozaeva, @ManuelNietoDom1, @maxi_prep et al. @ACSSynBio @LabNikel @DTUBiosustain #OpenAccess
pubs.acs.org/doi/10.1021/acs…
1
10
55
2,078
19 Dec 2024
Finally, this piece from @NicoKrink provides a guided overview of CRISPR-based editing tools for bacteria, offering orientation on their diverse applications, challenges, and improvements, inspired by The Hitchhiker’s Guide to the Galaxy
doi.org/10.1038/s44319-024-0…
1
3
123
19 Dec 2024
We hope this thread is helpful to everyone interested in synthetic biology and metabolic engineering, or simply curious about what our group has been up to in 2024. Have some relax in this break to charge your energies back! 🎉
3
107
19 Dec 2024
And also Meliawati led this work on Paenibacillus polymyxafor isobutanol production by genes KO and KI, optimizing NADPH regeneration, and identifying key metabolic bottlenecks, offering insights for further biotechnological applications of this bacterium
doi.org/10.1111/1751-7915.14…
1
1
109
19 Dec 2024
Touching on new bugs, here @enricoorsi and @dellavallesimo led the work for creating a new CRISPR/Cas toolkit for the emerging C1 platform C. necator, showing unprecedented efficiencies and turnover times for gene deletions
doi.org/10.1101/2024.11.24.6…
1
2
102
19 Dec 2024
@ManuelNietoDom1 explores here enzymatic synthesis of F-alanine enantiomers using few dehydrogenases, demonstrating efficient production with high enantiomeric excess, and introduces a novel fluorine biocatalysis method for trifluorinated alanine
nature.com/articles/s42004-0…
1
2
100
19 Dec 2024
And in this chapter, w/ @DeMaria_SynBio they outline use of L-threonine aldolases (LTAs) from E.coli and P.putida to efficiently synthesize 4-F-L-threonine in vitro protocols for adapting these activities to produce other fluorometabolites in vivo
10.1016/bs.mie.2024.02.016
1
1
96
19 Dec 2024
These types of sensors are ideal platform to harness for #evolution campaigns. Here, @enricoorsi led this #perspective article that proposes integration of ML, automation, high-throughput screenings and in vivo evolution to develop superior biocatalyst
doi.org/10.1038/s41467-024-4…
1
1
104
19 Dec 2024
Going back to P. putida, this minireview w/ @vdlorenzo_CNB and @PabloINik traces the evolution of KT2440 from a model organism for biodegradation studies to a SynBio #chassis, highlighting its adaptability and importance in various applications
doi.org/10.1128/jb.00136-24
1
1
89