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CRISP_SCIENCE @ScienceCrisp

RIKEN (CODATA, WDCM ...) - NIG (CODATA, DDBJ/CIB, WDCM, OECD BRC, GBIF, JST BIRD, TPRP ...) - FREE

Joined January 2017
  • Tweets 25,118
  • Following 351
  • Followers 2,014
3,438 Photos and videos
REVIEW "#CRISPR-tool box and its applications in tomato: From stress resilience to trait improvement" Subramanyam Reddy Chinreddy, Vaibhavi Patel, Swetha Kondala, Thejaswini Alavalapati, Celeste Gracia-Rodriguez, Umesh K Reddy. Plant Stress. 2026-06-06. doi.org/10.1016/j.stress.202…
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News & Views “Spatial #CRISPR screens map total RNA in tissue” Leon Schwartz, Pornchanan Pholraksa & Yogesh Goyal. Nat Biotechnol. 2026-06-11. doi.org/10.1038/s41587-026-0… Perturb-DBiT

Spatial CRISPR screens map total RNA in tissue

Nature Biotechnology - A method for spatial CRISPR perturbation screening reveals how genetic changes alter coding and non-coding RNA in a native tissue context, including the tumor microenvironment.

nature.com
CRISP_SCIENCE
@ScienceCrisp
Jun 13
"Large-scale, spatially resolved panoramic #CRISPR screening in native tissue environments using Perturb-DBiT" Alev Baysoy, Xiaolong Tian, Paul Renauer, Feifei Zhang [..] Sidi Chen, Rong Fan.   Nat Biotechnol 2026-06-11. doi.org/10.1038/s41587-026-0… Perturb-DBiT (perturbation-compatible deterministic barcoding in tissue); mRNA, lncRNA, miRNA, tRNA bioRxiv 2024-11-10 (Spatially Resolved in vivo CRISPR Screen Sequencing via Perturb-DBiT")
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NEWS "Phase 1 clinical trial set to begin on first leishmaniasis vaccine" Emily Cladwell. Ohio State News. 2026-06-02. news.osu.edu/phase-1-clinica… #CRISPR-Cas9 KO centrin Neglected Tropical Disease

Phase 1 clinical trial set to begin on first leishmaniasis vaccine

A phase 1 clinical trial testing the safety of a leishmaniasis vaccine is set to begin in the coming months – the first vaccine created to protect against the disfiguring skin disease common in...

news.osu.edu
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"Large-scale, spatially resolved panoramic #CRISPR screening in native tissue environments using Perturb-DBiT" Alev Baysoy, Xiaolong Tian, Paul Renauer, Feifei Zhang [..] Sidi Chen, Rong Fan.   Nat Biotechnol 2026-06-11. doi.org/10.1038/s41587-026-0… Perturb-DBiT (perturbation-compatible deterministic barcoding in tissue); mRNA, lncRNA, miRNA, tRNA bioRxiv 2024-11-10 (Spatially Resolved in vivo CRISPR Screen Sequencing via Perturb-DBiT")

Large-scale, spatially resolved panoramic CRISPR screening in native tissue environments using...

Nature Biotechnology - In vivo CRISPR genetic perturbations are spatially mapped at scale.

nature.com
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2026-06-13 2度と起こらないであろう事象
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"Improving iSpyMacCas9 multiplex genome editing in rice by CRISPR-combo-mediated BBM1 activation" Innocent Byiringiro, Danyel Fernandes Contiliani, Colin Davies, Filiz Gurel, Silvana Creste, Yiping Qi. Plant J. 2026-06-07. doi.org/10.1111/tpj.70980 #CRISPR-Combo technology enables simultaneous targeted mutagenesis and transcriptional activation in plants … applied to rice by replacing SpyCas9 (5'-NGG-3’ PAM) with iSpyMacCas9 (5'-NAAR-3' PAM)

Improving iSpyMacCas9 multiplex genome editing in rice by CRISPR‐combo‐mediated BBM1 activation

This study establishes iSpyMacCas9-Combo as an expanded CRISPR-Combo platform that enables efficient simultaneous genome editing and transcriptional activation in rice, particularly at AT-rich or...

onlinelibrary.wiley.com
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“Optimized protocol for efficient generation, confirmation, transformation, and #CRISPR editing of grapevine hairy roots” Sasha M Tietz, Kyle Brenner, Tumelo Moyo, Philip R Young, Melané A Vivie. J Exp Bot. 2026-04-02. doi.org/10.1093/jxb/erag165

Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of...

Optimized workflows and methods are provided for the establishment of Rhizobium rhizogenes-mediated transgenic or CRISPR-edited hairy root cultures for gra

academic.oup.com
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"Single-cell multi-omic atlas and morphogen screening informs midbrain and hindbrain organoid engineering" Nadezhda Azbukina, Zhisong He, Hsiu-Chuan Lin [..] J. Gray Camp, Barbara Treutlein. [ 10]. Nat Neurosci 2026-06-03. doi.org/10.1038/s41593-026-0… … Together, the multi-omic atlas and morphogen screen reveal morphogen–regulon relationships guiding region-specific progenitor differentiation towards diverse neuron types of the posterior brain. #CRISR-Cas9 KO

Single-cell multi-omic atlas and morphogen screening informs midbrain and hindbrain organoid...

Nature Neuroscience - By combining single-cell sequencing and morphogen screening, researchers map how signaling cues shape cell identity in mid-hindbrain organoids and identify conditions that...

nature.com
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“Selection of human hematopoietic stem cells bearing the intended functional edit by transient AND-gate reporters" Daniele Canarutto, Martina Fiumara [..] Samuele Ferrari, Luigi Naldini [ 17]. Nat Biotechnol 2026-06-01. doi.org/10.1038/s41587-026-0… SMArT (Selection by Means of Arificial transcriptional activators) strategies may broaden the therapeutic applicability of gene-sized editing while reducing its genotoxic burden.

Selection of human hematopoietic stem cells bearing the intended functional edit by transient...

Nature Biotechnology - Selection increases the purity of blood stem cells bearing targeted homologous recombination.

nature.com
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応用技術は飛行機だ。基礎研究は滑走路だ。 人々は飛行機は見るが、滑走路は目に入っていても見てはいない。 crisp-bio.blog.jp/archives/3…

基礎研究への投資を３分間で納得させることが可能か？ - 基礎科学が世界を変えたのに : crisp_bio

2026-06-14「基礎研究への投資を３分間で納得させる」のに使えそうな表現として、ここで、ピッツバーグ大学のTakashi D.Y. Kozai教授（生物工学）のBluesky投稿 (@bioniclab.bsky.social 2026-06-09) を引用しておきます [右図はスレッドの冒頭のスクリーンショット]。なお、

crisp-bio.blog.jp
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ファージは、科学者が発明する前から、sgRNAを発明していたらしい crisp-bio.blog.jp/archives/4… #CRISPR

バクテリオファージは、2020年ノーベル化学賞共同受賞者に先んじて、sgRNAを発明していたようです : crisp_bio

　CRISPR-Cas9 GE技術は、天然には存在しなかった人工的なsgRNAを創出したことで、実現されました。sgRNAは、自然界では細菌やアーケアのゲノムに内在しているCRISPRアレイからプロセスされてくるCRISPR RNA (crRNA）とtracRNAを融合させた人工物です。今回、ドイツ・スイス

crisp-bio.blog.jp
CRISP_SCIENCE
@ScienceCrisp
Jun 11
"Phage-encoded single-guide RNAs subvert #CRISPR-Cas9 immunity" Maximilian Feussner, Angela Migur, Jinquan Li [..] Zasha Weinberg, Rafael Pinilla-Redondo, Peter C. Fineran, Chase L. Beisel [ 5] bioRxiv 2026-06-02 (preprint). doi.org/10.64898/2026.06.01.… Central to CRISPR technologies is the single-guide RNA (sgRNA), an engineered fusion of a processed CRISPR RNA and tracrRNA. Bacteriophages encode similarly compact viral sgRNAs (vsgRNAs) that counteract bacterial CRISPR-Cas9 immunity. Even: vsgRNAs can enable genome editing in mammalian cells, offering a natural guide RNA template for CRISPR technologies.
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"Phage-encoded single-guide RNAs subvert #CRISPR-Cas9 immunity" Maximilian Feussner, Angela Migur, Jinquan Li [..] Zasha Weinberg, Rafael Pinilla-Redondo, Peter C. Fineran, Chase L. Beisel [ 5] bioRxiv 2026-06-02 (preprint). doi.org/10.64898/2026.06.01.… Central to CRISPR technologies is the single-guide RNA (sgRNA), an engineered fusion of a processed CRISPR RNA and tracrRNA. Bacteriophages encode similarly compact viral sgRNAs (vsgRNAs) that counteract bacterial CRISPR-Cas9 immunity. Even: vsgRNAs can enable genome editing in mammalian cells, offering a natural guide RNA template for CRISPR technologies.

Phage-encoded single-guide RNAs subvert CRISPR-Cas9 immunity

Central to CRISPR technologies is the single-guide RNA (sgRNA), an engineered fusion of a processed CRISPR RNA and tracrRNA that directs Cas9 and many Cas12 nucleases to bind and cleave target...

biorxiv.org
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"A census of anti-CRISPR proteins reveals AcrIE9 and AcrIE13 as inhibitors of the Escherichia coli K12 type IE #CRISPR-Cas system" Dmitry Taranenko [..] Konstantin Severinov, Ekaterina Semenova, Artem Isaev [ 11]. Proc Natl Acad Sci U S A. 2026-06-08/06-16. doi.org/10.1073/pnas.2529903…

A census of anti-CRISPR proteins reveals AcrIE9 and AcrIE13 as inhibitors of the Escherichia coli...

CRISPR-Cas adaptive immunity systems provide defense against mobile genetic elements and are often countered by diverse anti-CRISPR (Acr) proteins....

pnas.org
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"Integrated Chronic In Vivo and In Vitro Screens Uncover NFIL3 as a Driver of T-cell Dysfunction" Nayan Jain, Yuzhe Shi [..] Judith Feucht, Michel Sadelain [ 12] Cancer Discovery, 2026-02-27. doi.org/10.1158/2159-8290.cd… This study presents a two-step screening framework, integrating an in vivo pooled guide RNA screen with a multiparameter, in vitro arrayed screen. #CRISPR-Cas9 GE

Integrated Chronic In Vivo and In Vitro Screens Uncover NFIL3 as a Driver of T-cell Dysfunction

AbstractChimeric antigen receptor (CAR) therapy has transformed the treatment landscape for hematologic malignancies, but its efficacy in solid tumors is limited, owing in part to insufficient...

aacrjournals.org
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“Genome-scale perturb-seq in primary human CD4 T cells maps context-specific regulators of T cell programs and human immune traits" Ronghui Zhu, Emma Dann [..] Jonathan K. Pritchard, Alexander Marson [ 6] bioRxiv 2025-12-24 (preprint). doi.org/10.64898/2025.12.23.… #CRISPR-i Web site - virtualcellmodels.cziscience…

Genome-scale perturb-seq in primary human CD4 T cells maps context-specific regulators of T cell...

Gene regulatory networks encode the fundamental logic of cellular functions, but systematic network mapping remains challenging, especially in cell states relevant to human biology and disease. Here,...

biorxiv.org
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"A #CRISPR/Cas9-based genome-editing platform enabling efficient and precise gene replacement in Lipomyces starkeyi" Rikako Sato [..] Hiroaki Takaku [ 6]. FEMS Yeast Res. 2026-01-05. doi.org/10.1093/femsyr/foag0… … in a Cas9-expressing Δlslig4 strain with suppressed NHEJ, precise gene replacement was achieved with 100% accuracy using 50-bp homology arms.

A CRISPR/Cas9-based genome-editing platform enabling efficient and precise gene replacement in...

A CRISPR/Cas9 genome-editing platform in Lipomyces starkeyi enables efficient and precise gene replacement with minimal homology by combining Cas9-induced

academic.oup.com
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"Uncovering functional variants using a high-efficiency PE3-based screening platform" Shisheng Huang, Wanyu Tao [..] Shang-­ Min Zhang, Xingxu Huang. Sci Adv. 2026-06-05. doi.org/10.1126/sciadv.ady73… ＃CRISPR
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NEWS "World-first: therapy to make cells young again trialled in a person" Heidi Ledford. Nature 2026-06-09. doi.org/10.1038/d41586-026-0…

World-first: therapy to make cells young again trialled in a person

Nature - A participant in a landmark clinical trial has been given a cellular-reprogramming treatment that aims to rejuvenate damaged cells in the eye.

nature.com
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"Life Biosciences Announces First Patient Dosed in Phase 1 Trial of ER‑100 for Optic Neuropathies" Life Biosciences. 2026-06-09. lifebiosciences.com/life-bio… ER‑100 is designed to restore function in retinal ganglion cells using Life Biosciences’ Epigenetic Restoration platform, which utilizes controlled expression of three transcription factors, OCT4, SOX2 and KLF4 (OSK), to reset cellular gene expression patterns and restore cells to a more youthful and functional state.

Life Biosciences Announces First Patient Dosed in Phase 1 Trial of ER‑100 for Optic Neuropathies

Lead epigenetic restoration therapy enters the clinic for glaucoma and NAION, targeting a root cause of age-related vision loss BOSTON, June 9, 2026 — Life Biosciences, Inc. (“Life Bio”), a biotech...

lifebiosciences.com
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"Reprogramming to recover youthful epigenetic information and restore vision" Yuancheng Lu [..] David A Sinclair. Nature 2020-12-02/Dec. doi.org/10.1038/s41586-020-2…

Reprogramming to recover youthful epigenetic information and restore vision

Nature - Expression of three Yamanaka transcription factors in mouse retinal ganglion cells restores youthful DNA methylation patterns, promotes axon regeneration after injury, and reverses vision...

nature.com
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