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Murphy Lab @_Murphy_Lab

Structural and mechanistic study of proteins, especially redox- and metalloproteins

Joined September 2021
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  • Followers 456
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Happy to share that our work on the FDX2-bound core ISC complex is now published in @NatureComms 🎉 nature.com/articles/s41467-0… By the way, we are now also on @bluesky! 🦋 bsky.app/profile/bjmurphylab…

Two-stage binding of mitochondrial ferredoxin-2 to the core iron-sulfur cluster assembly complex

Nature Communications - De novo [2Fe-2S] cluster synthesis by the mitochondrial ISC complex requires electrons from ferredoxin-2 (FDX2). The authors map FDX2 binding to the core ISC complex,...

nature.com
Murphy Lab@_Murphy_Lab
23 Feb 2024
🎉 Our latest preprint is now online 👉 biorxiv.org/content/10.1101/… Congrats to @RalfSteinhilper who determined #cryoEM structures of the ferredoxin 2-bound core iron-sulfur cluster assembly complex under reducing and turnover conditions at 2 Å resolution. More below! 👇 🧵 1/8
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Our elemental mapping work, combining cryo-EM and EELS to REEL analysis, is now also published on Nature Methods: nature.com/articles/s41592-0… For a highlight “reel”, see here 👇

Murphy Lab@_Murphy_Lab
19 Jan 2024
Happy to announce that our efforts to establish elemental mapping for cryo-EM ❄️ by EELS 🌈 are now shared on bioRxiv: biorxiv.org/content/10.1101/… Congratulations to @zoliviapg and co-authors! But wait - Can we map elements in 3D? 🧵👇 1/6
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How could CO₂ fixation occur in anaerobic and ancient life? Our new preprint sheds light on catalytic mechanism of the key enzyme CODH/ACS in the Wood-Ljungdahl pathway. Watch the summary movie to see how CO₂ is transformed into acetyl-CoA. Read more: t.ly/u3Ir0
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The story doesn’t end there. QM/MM calculations reveal novel features of the catalytic metalloclusters, the C-cluster and A-cluster. The results suggest an electron storage mechanism for the C-cluster, and the configuration of the A-cluster in the ligand-free resting stage.
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Congrats to @MaxYin_211013 and co-authors! Big thanks to X-ray team: Tristan Wagner, Olivier Lemaire and Mélissa Belhamri @MarineMicrobio; computational team: Gerhard Hummer and José Guadalupe Rosas Jiménez @HummerLab; MS team: Anna Shevchenko @mpicbg for the great collaboration!
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Happy to see our study on the structure of 46-kDa Streptococcus pneumoniae NOX out in @NatureSMB! Congratulations to the authors @PABLOSANSEGUND3 and @vicsnorr 🥳 Check it out here (open-access) 👉 t.ly/T0oej

Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase

Nature Structural & Molecular Biology - Using cryo-electron microscopy, the authors obtained structures of Streptococcus pneumoniae nicotinamide adenine dinucleotide phosphate (NADPH) oxidase...

nature.com
Murphy Lab@_Murphy_Lab
20 Oct 2023
Excited to share our preprint: “Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase” in which we obtained a structure of a 46-kDa membrane protein to 2.2 Å resolution without fiducials. tinyurl.com/zycuw3p6
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🎉 Our latest preprint is now online 👉 biorxiv.org/content/10.1101/… Congrats to @RalfSteinhilper who determined #cryoEM structures of the ferredoxin 2-bound core iron-sulfur cluster assembly complex under reducing and turnover conditions at 2 Å resolution. More below! 👇 🧵 1/8
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Our study clarifies how FDX2 binds to the core ISC complex and confirms that de novo FeS biosynthesis is a highly concerted process. This might have implications for understanding the concentration-dependent effects of frataxin in diseases like Friedreich’s ataxia. 🧵 7/8
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Many thanks to our colleagues @MPIbp and collaborators in the group of Roland Lill @Uni_MR for cooperation on this story and a study of the sulfur transfer intermediates in the frataxin-bound core ISC complex: doi.org/10.21203/rs.3.rs-348… 🧵 8/8

Mechanism and structural dynamics of sulfur transfer during de novo [2Fe-2S] cluster assembly on...

Maturation of iron-sulfur proteins in eukaryotes is initiated in mitochondria by the core iron-sulfur cluster assembly (ISC) complex, consisting of the cysteine desulfurase sub-complex NFS1-ISD11-A...

researchsquare.com
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Happy to announce that our efforts to establish elemental mapping for cryo-EM ❄️ by EELS 🌈 are now shared on bioRxiv: biorxiv.org/content/10.1101/… Congratulations to @zoliviapg and co-authors! But wait - Can we map elements in 3D? 🧵👇 1/6
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A big thanks for their contributions, especially to @_Higor_Rosa, as well as Dietmar Riedel, @YuSebyChen, @FilipPetegem, and the always-helpful team from CEOS whose awesome CEFID we have been relying on, as well as @DECTRIS_News whose ELA has been crucial to this project. 🧵 5/6
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More research will be needed to reach single-atom sensitivity. In the meantime, we’d be grateful for any feedback and hope you enjoy the read! Also, if you’d like to join the team: We do have an open post-doc position! ^^ recruitingapp-5577.de.umanti… 🧵 6/6
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