Joined September 2022
- Tweets 22
- Following 86
- Followers 47
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11 Photos and videos
Feb 25
Excited to share our preprint exploring the structural, biochemical and in vivo properties of DRT7/UG10 reverse transcriptases which produce dsDNA using a combination of primase and polymerase activities and provide broad-spectrum antiphage defense!
biorxiv.org/content/10.64898…
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Feb 25
E. coli DRT7 provides defense against a very broad range of phages and uses a direct defense mechanism. The escape mutants of phage lambda and Bas1 that overcame this protection carried mutations in genes encoding a DNA mimic and a putative phosphoesterase, respectively.
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Feb 25
I wish to thank all the co-authors from @IIMCB_Poland and from @hauryliuk lab at @lunduniversity ! @SOLARIS_science
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26 Jun 2025
I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.
biorxiv.org/content/early/20…
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26 Jun 2025
Finally, we deliver the first complete cryo-EM structure of a two-component recombinase composed of ICP8 annealase and UL12 alkaline nuclease. The complex was reconstituted using a model DNA with a dsDNA and 3′ ssDNA overhang.
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26 Jun 2025
I wish to thank all the coauthors of this study and our colleagues from Nowotny lab. We are also grateful to @IIMCB_Poland, @NCN_PL and @SOLARIS_science for making this project possible!
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26 Jun 2025
A very similar DNA conformation is observed in the crystal structures of ICP8 with 14-mer ssDNA. ICP8 subunits form dimers with a two-fold symmetry, with their DNA-binding sites facing each other and the outward-rotated bases of bound DNAs form pairs in a flexible manner.
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26 Jun 2025
We determined a cryo-EM structure of BALF2 bound to ssDNA at 3.2 Å resolution. The visible fragment of DNA is accommodated in a positively charged cleft. In the first four nucleotides, the base edges face the protein, but the next three bases are rotated away from the protein.
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26 Jun 2025
We are very happy to share the preprint of our latest study on the DNA-binding proteins from Herpes Simplex Virus-1 and Epstein-Barr Virus! @IIMCB_Poland
26 Jun 2025
Structural insights into DNA annealing and recombination by herpesviral DNA-binding proteins ICP8 and BALF2 biorxiv.org/content/10.1101/… #biorxiv_molbio
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11 Apr 2024
Following up on our paper about AbiK and Abi-P2, we now present an in-depth characterization of the third Abi polymerase, AbiA, performed in collaboration with @hauryliuk lab and @DziembowskiLab.
academic.oup.com/nar/advance…
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21 Apr 2023
Great job, congratulations to Shivlee and Mariusz!
21 Apr 2023
Very glad to share that our work on the RecFOR pathway is finally published in @NatureSMB! @MarcinNowotny @IIMCB_Poland @MariuszCzC. Many thanks @SOLARIS_science @NCN_PL #cryoEM. (1/2)
doi.org/10.1038/s41594-023-0…
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Małgorzata Figiel retweeted
7 Dec 2022
➡We are starting recruitment for #Warsaw4PhD within @IIMCB_Poland
➡We offer the opportunity to join 2️⃣ unique projects under the guidance of outstanding experts:
prof.@MarcinNowotny dr #MalgorzataFigiel & prof. @gmichlew
👉Recruitment details: bit.ly/3VCRmYX
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8 Dec 2022
So happy that I was granted funding for this awesome project!
8 Dec 2022
💥Congrats to @drfigiel from @IIMCB_Poland on getting new research grant financed by @NCN_PL on DNA replication process in herpesviruses‼
👏💪This is a great success, we are so proud of!
And herpesviruses - beware❗😉
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Małgorzata Figiel retweeted
17 Sep 2022
Ever heard of trimeric reverse transcriptases? Check out our new paper from @MarcinNowotny lab with cryo-EM and MX structures of Abi polymerases. Congratulations to @drfigiel and Marta!
#cryoEM @IIMCB_Poland @t_goral @SOLARIS_science
doi.org/10.1093/nar/gkac772
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