9 May 2024
When one of you posts the results of what you think needs to be checked, I'll pay attention but it appears neither of you have run this until you were called out for not having any familiarity with the tool.
Yes.. You can select RevComp but its still ssDNA and too large as dsDNA
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30 Dec 2022
Interesting piece on Intel’s zettaflops roadmap. They are planning to operate future cores near threshold, which is just what the analysis we did this year predicted was the optimal operating voltage. Good start, guys, but you can do even better! #RevComp seekingalpha.com/article/456…
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7 Jul 2022
Here is another classic Gaff from Funk.
Dr. Lee demonstrates Funk can't read an electropherogram. No Funk.. When you Sanger sequence from a forward read, you do not get a trace that include the forward primer. Only the revcomp of the reverse primer at the 3' end.
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25 Feb 2022
fair warning - the first version is going to be revcomp only, that our version of "hello world"
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6 Feb 2022
I got @detahq-pilled in the summer last year. Should have told you about my little revcomp service I built there earlier! apollo.deta.dev/revcomplemen…
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4 Jan 2022
The revcomp situation on barcode sequences input to bcl2fastq is a good reflection of the state of core bioinformatics software usability
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3/ look at the sequences - can you see the polyA tail? (stretches of Ts or As), can you identify the 5' and 3' cDNA primer? Here's an example from UHRR Iso-Seq data where you can see two reads with the polyA tail (as Ts), so we know these reads are revcomp of transcript
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20 Feb 2021
Have some more detailed questions on the architecture choices particularly how you're dealing with revcomp. Will send a more detailed email. 2/
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Just added this to my bash profile:
function revcomp {
echo $1 | tr "ACGT" "TGCA" | rev
}
2 Dec 2020
The reverse complement of a sequence of nucleotides can be found on a terminal using the 'tr' and 'rev' bash commands #openmethods #minimethods
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13 Jan 2020
Cutadapt 2.8 just released. It comes with a --revcomp option: This checks both the read and its reverse complement for an adapter and swaps it if necessary. Changelog at cutadapt.readthedocs.io/en/l…
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10 Nov 2019
Had punt on BOTB because of your win then fecking realised it was revcomp 🙈🙈
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17 Aug 2019
Always wanted to Polymerase methyl incorp one strand of DNA tethered to its native revcomp and run it through pores. Could do methyl A, Methyl C, Inosine and a variety of analogs that break up 2ndary structure and provide better error correction.
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15 Apr 2019
We are so excited to be apart of the Entrepreneur competition! Congratulations to Speech Cloud & all of the contestants for their hard work! #revcomp instagram.com/p/BwS1v0ahllN/…
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13 Apr 2019
Good times had by all at the 2nd Annual RevRoad Entrepreneur Competition. Thanks to all our sponsors, judges, staff, and participants! What a great day! See you next year. @revroadutah #RevComp .
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13 Apr 2019
2nd place...SpeechCloud. Well done! $2500K! Big Thank You to @fourfoodsgroup for sponsoring this award. #RevComp @revroadutah
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13 Apr 2019
Meet last year’s $10K RevRoad Entrepreneur Competition winner, @journeyfrontinc. Who will be the winner this year? #RevComp
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