🧬 A new report in Science Advances highlights how nonstop-mutations can alter translation initiation Point mutations can affect all levels of gene expression, leading to dysregulation and cancer. In a new study published in Science Advances, Pal et al. explore the effect of nonstop-mutations in VHL, a tumor suppressor gene frequently involved in kidney cancer. VHL uses two translation initiation sites to generate two different isoforms. Wild type cells predominantly use the second start codon giving rise to the abundant, short isoform. The introduction of a nonstop mutation favors the use of the first start codon, shifting the balance to the longer VHL isoform. Read the full paper here: science.org/doi/full/10.1126… ☑️ Using siPOOLs and a custom raPOOL probe set - specifically designed for VHL – the authors were able to affinity purify both wild-type and nonstop-mutated VHL mRNA, and identify differentially bound protein factors that may trigger the changed preference of the ribosome.

Exciting Update! We’re pleased to announce Aurogene Srl as our new exclusive distributor in Italy. 📍 If you’re based in Italy, feel free to connect with Aurogene Srl for all your needs and questions regarding RNAi, RNA-Seq and Ribo-Seq. Welcome to the siTOOLs Biotech GmbH Biotech network, Aurogene Srl ! 💙 #officialdistributor #Italy #RNAi #RNA-Seq -- Siamo lieti di annunciare che Aurogene Srl è il nostro nuovo distributore esclusivo in Italia. 📍 Se avete sede in Italia, non esitate a contattare Aurogene Srl per tutte le vostre esigenze e domande su RNAi, RNA-Seq e Ribo-Seq. Benvenuto nella rete siTOOLs Biotech, Aurogene Srl 💙

Happy International Day of Women and Girls in Science!👩‍🔬 From groundbreaking researchers to creative problem-solvers, women continue to drive innovation and progress across all scientific fields. At siTOOLs Biotech, we celebrate and support their invaluable contributions to research and technology. #WomenInScience #STEM

Minimize your RNA contaminants by using the RiboCut Marker. The RiboCut Marker is a high-resolution molecular marker, which facilitates precise excision of 30-mer ribosome-protected fragments (RPFs) from preparative gels. More info: buff.ly/4doaX7H #RiboSeq #RNAresearch #RNASeq

riboPOOL of the month: Pan-Archaea riboPOOL!🧡🧫 Efficient rRNA depletion is essential for archaeal transcriptomics. The Pan-Archaea riboPOOL enables comprehensive RNA-Seq analysis by selectively removing rRNA across diverse archaeal species, enhancing the detection of low-abundance transcripts and improving data quality. 📌Learn more: buff.ly/3Q5lGKF #rRNAdepletion #Archaea #Metatranscriptomics #RNASeq

New publication investigates the dose-dependent antibacterial effects of the xanthine oxidase-lactoperoxidase system on infant oral microbiota. Using riboPOOLs for E. coli rRNA removal, researchers reveal global gene expression changes, offering insights into its microbial impact. Read more: buff.ly/4gh1csS #microbiome #rRNAdepletion

Exclusive offer on the RBP siPOOL Library!🎉 Covering 1036 RBP genes with the latest annotations, this library is available at 0.1 nmol per gene at a special price. Request your quote today: buff.ly/3ZXwf8m

New research highlights distinct mechanisms regulating Tau monomer and aggregate uptake in human neurons, shedding light on neurodegenerative processes. Using siPOOLs for gene knockdown, this study offers deeper insights into Tau pathology. Read more: buff.ly/3DTQbAl #Neuroscience #siRNA #RNAi

Don't miss out on our winter promotion. All 6 rxn riboPOOL kits for 49 €! Request a quote here: buff.ly/3Cf5x1n . *Promotion valid until Feb. 28th.🧡 #rRNAremovalkit #RNASeq

Starting the year strong with our riboPOOL of the month: The Plant riboPOOL! 🌱Efficiently deplete rRNA in plant RNA samples for high-quality transcriptomic analysis. The Pan-Plant riboPOOL consistently removes >90% of rRNA from total RNA samples across various flowering plant species. Let your green discoveries grow! 🌾✨ Read more: buff.ly/4jbq5Jq #PlantResearch #RNASeq

Happy Holidays from the siTOOLs Team! 🎄🎇🥂 We look forward to working with you in 2025 and wish you a peaceful and prosperous new year!

Wrap Up the Year with Savings on the RBP siPOOL Library! 🎉 Covering 1036 RBP genes with the latest annotations, this library is available at 0.1 nmol per gene at a special price. Offer valid for inquiries submitted by December 31st. Request your quote today: buff.ly/3ZXwf8m

The #HIRI & @Uni_WUE's Faculty of Medicine jointly open a call for 3 HELMHOLTZ RESEARCH GROUPS WITH JUNIOR PROFESSORSHIPS (W1) in the areas of 🔹 RNA-based Infection Research 🔹 Microbial RNA Biology 🔹 RNA-based Medicine Apply by January 19, 2025 at helmholtz-hiri.de/en/w1-grou…

Tired of the tediousness of manual sample fractionation? With the siFractor, you can overcome this limitation and much more. The siFractor offers semi-automated tube fractionation, eliminating errors and enhancing precision. More information here: buff.ly/41rdwmF

Struggling to find an rRNA depletion solution for your species? Custom riboPOOLs provide tailored solutions to efficiently remove unwanted rRNAs and other abundant RNAs from any organism. Request a quote today: buff.ly/49twye6

Don't miss out on the last days of our end-of-the-year promotion 🎁: 6 rxn riboPOOL kits for 49 €, and 25% off all siPOOLs 5 nmol. Request a quote here: buff.ly/3BehiFo

riboPOOL of the month🧡. The Human Holobiont riboPOOL is one of our readily available combination riboPOOLs, designed to efficiently deplete ribosomal RNA (rRNA) from human and bacterial RNA samples. Learn more: buff.ly/3AXSD80

One of the key steps in library preparation for RNA-Seq is the removal of unwanted rRNAs. This step enhances data quality, focuses on meaningful transcripts, and helps reduce sequencing costs! For #ribodepletion check out our available riboPOOL kits: buff.ly/4ikZY2e

Don't miss out on our coming up end-of-the-year promotion. 25% off all siPOOLs 5 nmol! Request a quote here: buff.ly/3OjIu8y . *Promotion valid from Dec.1st to 15th.💙 #RNAi #siRNA

Excited to share a recent publication using our raPOOLs: LINC01021 Attenuates Expression and Affects Alternative Splicing of a Subset of p53-Regulated Genes. Thank you for choosing raPOOLs for targeted RNA capture! Read more: buff.ly/3Z8zI3s #GeneRegulation #p53 #RNAResearch