Sharing speeds science! Addgene helps scientists around the world find and share research materials - plasmids, viral preps, antibodies, data, and more.

Joined October 2009
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11 Jan 2024
Addgene is no longer monitoring or using our X, formerly Twitter, account. Instead, find @addgene on Instagram, TikTok, Facebook, LinkedIn, and emerging microblogging platforms. You can read more about this decision here: blog.addgene.org/addgene-is-…
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🎊 Happy birthday, @Addgene! 🥳 Today we cell-ebrate you and the 137,289 plasmids you have sent on behalf of 5,822 labs from around the world!🤯 Get to know Addgene's #OpenScience mission: addgene.org
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Our Cell Line of the Week is SERCA2 - illuminating the sarcoplasmic/ endoplasmic reticulum! 💪 Browse our openly available hiPSC lines & plasmids – fluorescently-tagged to illuminate structures: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene
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4 Jan 2024
The key reagents from our recent CombiCell manuscript are now available on @Addgene addgene.org/Omer_Dushek/ @embojournal embopress.org/doi/full/10.10…

19 Jun 2023
Our new work! Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs biorxiv.org/content/10.1101/…
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Working with @addgene to make all plasmids easy to get soon
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Our #CellLineOfTheWeek is ras-related protein - targeting endosomes! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience
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14. Get your SOXmas presents @Addgene addgene.org/browse/article/2…

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Most of our pHYX plasmids recently published doi.org/10.1016/j.ymben.2023… are now available @Addgene (addgene.org/browse/article/2…). And merry Christmas to all! 🎅🎄 @ECCP_lab
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We've previously reported PAM variable PEmax for correcting KRAS mutations, and now our constructs - PEmax-SpG, PEmax-SpG-P2A-hMLH1dn - are available on @Addgene! Check them out! #CRISPR #PrimeEditing addgene.org/browse/article/2…

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Troponin I (slow skeletal) is our #CellLineOfTheWeek! ❤️🔬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene. #OpenScience
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Perspective (OA): "The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene" liebertpub.com/doi/10.1089/c… >15,000 plasmids from >1,000 laboratories ∼40 countries 300,000 shipments to ∼5,000 organizations in ∼100 countries @Addgene @CRISPRchef
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14 Dec 2023
Haemophilus influenzae was the first bacteria to be sequenced but few genetic tools exist for this organism. It has small genome of only 1.8 Mbp and can be easily grown in lab. To speed up synbio research for this organism we created plasmids that now you can get @Addgene
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12 Dec 2023
You can find us on: Instagram: hubs.la/Q02cTfDN0 TikTok: hubs.la/Q02cT7wG0 Facebook: hubs.la/Q02cT7pp0 LinkedIn: hubs.la/Q02cTctc0 Addgene Blog: hubs.la/Q02cT8570
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Click editor plasmids now available on @Addgene addgene.org/browse/article/2… 🧬🖱️ Looking forward to feedback from the community on how well click editing works in your hands! @FerreiraSilvaJ @connorjtou @EmilyM_King @Madeline_Eller_ @Linyuan79834832 @DavidRufinoRam1

We’re excited to share our work developing a new gene editing approach that we’re calling #clickediting 🖱️✴️🧬. Click editors (CEs) utilize DNA-dependent polymerases to write bespoke edits into the genome. biorxiv.org/content/10.1101/… @CGM_MGH @MassGeneralNews @MGH_RI @harvardmed
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The plasmids are available on @Addgene, including recombinases, reporters, and empty vectors for your own favorite gene: addgene.org/browse/article/2…

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Interested in Transcription Factor high-content screening? we have a dFLASHy new platform for you. dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Plasmids @Addgene biorxiv.org/content/10.1101/…
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Addgene retweeted
Our #CellLineOfTheWeek is sialyltransferase 1 - targeting the golgi! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience
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Update: most of the Contact-FP probes are now available on @Addgene. We hope folks will try them out and let us know what you think! addgene.org/Sarah_Cohen/

Preprint alert! We are happy to introduce Contact-FP, a suite of probes for studying #MembraneContactSite dynamics. This was an amazing team effort led by Research Associate @GregoryMiner18 and postbacc Sidney Smith 1/11 biorxiv.org/content/10.1101/…
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🌈plasmids now available at @addgene 🍭 addgene.org/Arnaud_Gautier/

Very happy to share a new approach we developed at @institut_curie/@CNRS with my colleague and friend Arnaud Gautier from @Sorbonne_Univ_/@ENS_ULM CATCHFIRE allows to induce and sense protein interaction using a reversible fluorogenic system. More here : rdcu.be/dkAMo
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