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Pilhofer Lab @PilhoferLab
Joined July 2017
  • Tweets 113
  • Following 65
  • Followers 2,438
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Pilhofer Lab@PilhoferLab
17 Oct 2024
Captain Aureispira - a bacterial pirate ship🦜🏴‍☠️🪝- boarded @ScienceMagazine. Check out the manuscript here: science.org/doi/10.1126/scie… and have a look on our extended thread below ⬇️We provide new insights on bacterial predation from molecules ⚛️to ocean samples 🌊
Pilhofer Lab@PilhoferLab
30 Jan 2024
Bacteria can be pirates too... 🏴‍☠️ Let us introduce you to Captain Aureispira ⛵️ A bacterial pirate ship loaded with canons 💉 and grappling hooks 🪝🌴predating on other bacteria 🦠 Check the preprint: biorxiv.org/content/10.1101/… #predation #T6SS #T9SS #cryoEM #cryoET #teamtomo 🧵1/6
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Pilhofer Lab retweeted
D-BIOL @ ETH Zurich@ETH_DBIOL
20 Nov 2024
In a collaboration between the @PilhoferLab and the Albers Lab from the University of Freiburg, the researchers show that haloarchaea express contractile injection systems – or “molecular daggers”, which influence the microbial communities they inhabit. biol.ethz.ch/en/news-and-eve…
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Bye bye X/Twitter! Please come visit us on Bluesky @pilhoferlab.bsky.social bsky.app/profile/pilhoferlab… for future lab news, including a new paper on an Archaeal Type 6 Secretion System!
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ETH Zurich@ETH_en
18 Oct 2024
Researchers at ETH Zurich have analysed down to the smallest detail the unusual arsenal of weapons that a predatory marine bacterium has at its disposal. Perhaps one day these weapons could also be put to use in medicine. ethz.ch/en/news-and-events/e…

Catching prey with grappling hooks and cannons

Researchers at ETH Zurich have analysed down to the smallest detail the unusual arsenal of weapons that a predatory marine bacterium has at its disposal. Perhaps one day these weapons could also be...

ethz.ch
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Bacteria can be pirates too... 🏴‍☠️ Let us introduce you to Captain Aureispira ⛵️ A bacterial pirate ship loaded with canons 💉 and grappling hooks 🪝🌴predating on other bacteria 🦠 Check the preprint: biorxiv.org/content/10.1101/… #predation #T6SS #T9SS #cryoEM #cryoET #teamtomo 🧵1/6
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But does this happen in the environment? 🌍 With the help of @NBartlau and @MartinPolz, we analyzed a time-resolved metagenomic dataset from the ocean 🌊 and indeed found coupled dynamics indicating a predator-prey relationship between Vibrionaceae and ixotrophic predators 🧵6/6
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The peer-reviewed version of the manuscript can be found here: science.org/doi/10.1126/scie… 🙏 We thank the reviewers for their constructive feedback!

Mechanism of bacterial predation via ixotrophy

Ixotrophy is a contact-dependent predatory strategy of filamentous bacteria in aquatic environments for which the molecular mechanism remains unknown. We show that predator-prey contact can be...

science.org
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Fly-fishing for flagella... Check out the lovely perspective by Andrew Lovering and @SimonCaulton 🙏🙏 about our findings on bacterial predation via ixotrophy. science.org/doi/10.1126/scie…

Fly-fishing for flagella

Molecular grappling hooks are the tools of a sticky bacterial predator

science.org
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Exciting news!🚀 We have a new follow-up on the cytoplasmic Contractile Injection System from #Streptomyces!💉@casub1, in a wonderful collaboration with @JWSallmenII & @SusanSchlimpert, unveils the critical role of CisA in Streptomyces CIS functionality. ☠️🧵 1/2
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Read the preprint here: biorxiv.org/cgi/content/shor… to see how we used multidisciplinary approaches like #genetics 🧬, #cryoET ❄️🔬, single-particle #cryoEM, #lightmicroscopy 💡, and functional assays to unravel the secrets of this fascinating antibiotic-producing bacterium. 💊 🧵2/2
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An accompanying paper from the Matos lab in collaboration 👯‍♀️👯 with us and the @pedrobeltrao lab and Aebersold lab at @Dev_Cell unveils coordinated waves 🌊🌊🌊 of protein expression and phosphorylation in yeast gametogenesis. cell.com/developmental-cell/…

Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogene...

Wettstein et al. employ integrated proteomics and cryoET to study the process of meiosis in yeast and report that sequential waves of protein phosphorylation rewire the proteome during gametogenesis....

cell.com
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To #TeamTomo ❄️🔬: Do you also sometimes see strange filaments in your #cryoET data and don’t know what they are? 🤔 Try #FilamentID! 🪄🤓 Check out our new paper at @CellCellPress by @HugenerJannik and @xujw_thu together with the Matos lab cell.com/cell/fulltext/S0092… 🧵(1/5)
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Using #FilamentID, we found that the conserved aldehyde dehydrogenase 4 (Ald4) forms filaments in the mitochondria, whereas acetyl-CoA synthetase 1 (Acs1) polymerizes into nuclear and cytoplasmic filaments 🔍 🧵(4/5)
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What are the functions of these filaments? Structural characterization and functional assays suggest that Acs1 filaments facilitates the recovery of chronologically aged spores 😴and, more generally, the cell cycle re-entry of starved cells. 🥳🧵(5/5)
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#cryoET as discovery tool ❄️🔬: DPANN archaea are enigmatic, hard to cultivate, and rely on an ectosymbiotic lifestyle. Check out our new paper @ISMEJournal from Vasil Gaisin in collaboration with @Archaellum where we describe two DPANN lifecycle stages: academic.oup.com/ismej/advan…
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Free DPANNs are motile, but ectosymbiotic cells are linked with the host via an elaborate attachment organelle, making them the potentially first microorganisms using a straw 🥤 Also have a look at the beautifully complementing story from @DebnathGhosal: biorxiv.org/content/10.1101/…

A large attachment organelle mediates interaction between a novel Nanobdellota archaeon YN1 and its...

DPANN archaea are an enigmatic superphylum that are difficult to isolate and culture in the laboratory due to their specific culture conditions and apparent ectosymbiotic lifestyle. Here we success...

biorxiv.org
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Have you ever had nightmares of killer archaea? 🔪☠️😱 stop dreaming! Have a look at our latest preprint on how Halogeometricum borinquense uses it type 6 secretion system to kill Haloferax volcanii: biorxiv.org/content/10.1101/…

Archaeal type six secretion system mediates contact-dependent antagonism

Microbial communities are shaped by cell-cell interactions. Even though archaea are often found in associations with other microorganisms, the mechanisms structuring these communities are poorly...

biorxiv.org
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Pilhofer Lab retweeted
Pilhofer Lab@PilhoferLab
5 Feb 2024
It's out! It's out! 📢The bacterial Tc Toxins are out😱☠️ (and our paper 📜😉)! But how did these huge complexes get out? Where did they assemble? 🧐 Check out Tc Toxins' great escape from Yersina 🦠 @NatureMicrobiol: nature.com/articles/s41564-0… #cryoET #toxins #microbiology 🧵1/4
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Nature Microbiology@NatureMicrobiol
5 Feb 2024
See also the associated paper: Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations By Oleg Sitsel, Stefan Raunser & colleagues @oleg_sitsel @Intein nature.com/articles/s41564-0…

Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulat...

Nature Microbiology - A specialized subpopulation of Yersinia entomophaga cells uses a RoeA-regulated, temperature- and pH-dependent type 10 secretion system to mediate lytic Tc toxin release.

nature.com
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Congrats to @miki_hannah and to all collaboration partners.
Nature Microbiology@NatureMicrobiol
5 Feb 2024
OUT NOW 📢: Stepwise assembly and release of Tc toxins from Yersinia entomophaga By Miki Feldmüller, Martin Pilhofer & colleagues. @mikihannah @PilhoferLab @ETH_en #cryoET #teamtomo nature.com/articles/s41564-0…
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