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Filippo Caschera @Stjle

Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.

Joined April 2010
  • Tweets 1,490
  • Following 838
  • Followers 534
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Filippo Caschera
@Stjle
19 Feb 2025
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery sciencedirect.com/science/ar… #CellFree #SynBio
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Automated, modular assembly of reconstituted cell-free systems from in vitro-produced components cell.com/trends/biotechnolog…

Automated, modular assembly of reconstituted cell-free systems from in vitro-produced components

We developed Purified components Optimized for Flexible protein expression using in vitro-produced translation factors, an automated platform that entirely decouples translational machinery product...

cell.com
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Gene-driven Self-morphing Microtubule-based Active Matter researchsquare.com/article/r…

Gene-driven Self-morphing Microtubule-based Active Matter

Materials that change shape or flow typically rely on external fields, photochemical cues, or compositions fixed at the time of assembly. Here, we introduce a self-morphing active material whose...

researchsquare.com
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Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles cell.com/trends/biotechnolog…

Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles

Extracellular vesicles are cell-derived particles that can have therapeutic effects. To this end, we developed a cell-free extracellular vesicle engineering workflow that can be used to quickly...

cell.com
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Filippo Caschera retweeted
Polly Fordyce@fordycelab
Jun 3
Characterizing AI-designed proteins requires quantitative biochemistry at massive scale. Enter Amplicon/Protein Bead Display (APB-Display), a fully in vitro platform that quantifies Kd's for >100,000 variants in <3 days (preprint link below!) @Stanford_ChEMH @czbiohub (1/n)
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First real-world cell-free distributed biomanufacturing project science.org/doi/10.1126/scia…

International multisite implementation of distributed cell-free protein biomanufacturing to advance...

Multisite deployment of distributed cell-free biomanufacturing expands access and equity in global health research.

science.org
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Filippo Caschera retweeted
Maerkl Lab@MaerklLab
May 29
Pao-Wan and co's paper is published now as well ! "A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications" nature.com/articles/s41467-0…

A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications

Nature Communications - T7 RNA polymerase is widely used in synthetic biology, but tools to precisely regulate it are limited. Here, authors engineer a modular T7 RNAP regulatory toolbox for...

nature.com
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Filippo Caschera retweeted
Kathrin Leppek@KatLeppek
May 26
🚨 1/5 Check out the 2nd preprint from our lab on how IRES-mediated translation of synthetic circRNAs is employed in cells and in cell-free translation extracts, a highly collaborative effort with Immagina & the labs of Anders Lund and CK Chen.
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Filippo Caschera retweeted
shimizu@musaibou
May 27
SynCell Asia initiativeです、アジアでも盛り上げていきましょー　nature.com/articles/s41587-0…

A framework for building a synthetic cell from the SynCell Asia Initiative

Nature Biotechnology - Building a living cell from scratch requires overcoming a bottleneck that has remained unresolved despite decades of progress: orchestrating the spatiotemporal integration of...

nature.com
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Filippo Caschera retweeted
Naohiro Terasaka　寺坂 尚紘@NaohiroTerasaka
May 26
プレプリントをbioRxivで公開しました！市販の無細胞翻訳系を用いて、タンパク質N末端に非天然アミノ酸を高効率に導入する系を作りました。この系で発現したビオチン化バインダーは精製せずに、BLIを用いた相互作用解析に利用できます。 @Okamoto_Yasu_CH との共同研究です。biorxiv.org/content/10.64898…

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PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins nature.com/articles/s41467-0…

PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins

Nature Communications - Building a self-replicating biochemical system remains a challenge in synthetic biology. Here the authors demonstrate that the cell-free protein synthesis PURE system can be...

nature.com
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Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications sciencedirect.com/science/ar…
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Filippo Caschera retweeted
shimizu@musaibou
May 25
とうとう民主化運動が始まってしまった　researchsquare.com/article/r…

A minimal‑component E. coli cell‑free transcription–translation system: implications for democrat...

In vitro transcription-translation (TX-TL) systems are central to synthetic biology as platforms for protein production outside living cells; however, most current protocols rely on multicomponent...

researchsquare.com
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Enhanced tRNA array method version 2 for simultaneous in vitro synthesis of 21 tRNAs sciencedirect.com/science/ar…
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Cell-Free Systems link.springer.com/rwe/10.100…

Cell-Free Systems

Cell-free protein synthesis (CFPS) represents a versatile and powerful platform for in vitro protein production, utilizing cell extracts or purified components containing cellular machinery essential...

link.springer.com
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Filippo Caschera retweeted
Riku Nagai@riku_nagai_edu
May 21
🚀 Our new ACS Synthetic Biology paper is out! We restored protein-primed DNA replication in commercial PURE systems by simply swapping the vendor-supplied energy mix for a homemade counterpart. 🧬⚡ pubs.acs.org/doi/10.1021/acs…

Protein-Primed DNA Replication is Restored in a Modified Cell-Free Protein Synthesis System

In vitro directed evolution in synthetic microcompartments can support the evolution of genes with functions beyond affinity. The main challenge in implementing this strategy is the need to incorpo...

pubs.acs.org
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Filippo Caschera retweeted
Olivier Borkowski@O_Borkowski
May 16
Temporal Programming of Cell-Free Transcription Using Orthogonal Enzyme-Responsive DNA Blockers doi.org/10.1021/acssynbio.6c…
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A two-step selection method for in vitro evolution of translational proteins biorxiv.org/content/10.64898…
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Filippo Caschera retweeted
bioRxiv Synthetic Biology@bioRxiv_synbio
May 9
Automated Synthetic Cell-based Screening for Designed Proteins with Emergent Functions biorxiv.org/content/10.64898… #biorxiv_synbio
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Membrane Composition Influences Expression Yield of Plant Cytochrome P450s in E. coli Lysate-Based Cell-Free Systems | ACS Synthetic Biology pubs.acs.org/doi/10.1021/acs…

Membrane Composition Influences Expression Yield of Plant Cytochrome P450s in E. coli Lysate-Based...

Plant cytochrome P450 enzymes are central to natural product biosynthesis, but remain difficult to express in microbial hosts due to their transmembrane nature. Lysate-based, cell-free expression...

pubs.acs.org
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Filippo Caschera retweeted
市橋　伯一　Norikazu Ichihashi@Nori_Ichihashi
May 8
これは進化実験だけで5000時間かかっている力作です。出てきた現象が「絶滅」というどう扱っていいやらよくわからないものだったので解析も大変手こずりました。
Ichihashi Lab Komaba@KomabaLab
May 8
この前卒業したD3湯川さんの論文がオープンになりました。プレスリリースもしました。自己複製RNAを高頻度希釈環境で長期継代したら、絶滅へ向かう進化がおきました。進化すればいいってものではないようです。 c.u-tokyo.ac.jp/info/news/to… academic.oup.com/mbe/article…
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