ALT Figure 1 Decreased mitochondrial size and volume in the gastrocnemius, soleus, and cardiac muscle of aged mice in SBF-SEM 3D reconstructions. (a, b) Representative SBF-SEM orthoslices for male murine gastrocnemius, (c, d) soleus, and (e, f) cardiac tissues. (a′, b′) 3D reconstructions of mitochondria (various colors) in gastrocnemius, (c′, d′) soleus, and (e′, f′) cardiac tissues from 3-month-old and 2-year-old mice overlaid on ortho slices. (a″, b″) Pseudo-colored individual mitochondria in gastrocnemius, (c″, d″) soleus, and (e″, f″) cardiac tissues identify micro-level changes. (g–x) Quantification of 3D reconstructions, with each dot representing the average for all mitochondria quantified for one mouse. (g) Mitochondrial volume in the gastrocnemius muscle from 3-month-old and 2-year-old mice and (h) mitochondrial volume distributed as the percent of total mitochondria to visualize relative heterogeneity. ...check figure online for more details

ALT Mentoring Keynote: An invited speaker who exemplifies mentoring by their impact on training scientists and scholars belonging to underrepresented groups, particularly racial and ethnic minorities. Awardee selected by the Minorities Affairs Committee. Antentor Hinton, Jr., Assistant Professor, Ernest E. Just Early Career Investigator, Department of Molecular Physiology and Biophysics, Vanderbilt School of Medicine Basic Sciences