๐๐พExcited to introduce ๐๐ฅ๐ผ๐ฟ - enabling scalable label-free images-based cell profiling for chemical/genetic screen via continuous spinning ultrafast imaging
๐ ๐๐ฅ๐๐ฃ๐ฃ๐๐ฃ๐ ๐ผ๐ง๐ง๐๐ฎ๐๐ ๐ฟ๐๐จ๐ (๐๐ฅ๐ผ๐ฟ)
- Optofluidic 96-chamber circular array design supporting continuous live-cell imaging
- Ultrafast quantitative phase imaging (QPI) at 1000โs rpm โ sub-cellular-resolution label-free imaging across 100 cm2
- Disk design is fully compatible with standard protocols
๐ ๐๐๐จ๐จ๐๐ซ๐-๐๐๐๐ก๐ ๐ผ๐ฃ๐๐ก๐ฎ๐ฉ๐๐๐จ:
- 10 GB/sec real-time processing
- *InMorph* profiling strategyโ Rich biophysical signatures from label-free data
- Easily scalable to 100s of TBs for CRISPR/drug screens
โ
๐๐ช๐ก๐ฉ๐-๐๐๐๐๐ฉ๐๐ ๐๐๐ก๐๐๐๐ฉ๐๐ค๐ฃs
- Mechanical stability testing
- Cell health assays
- Transcriptomic analysis
We demonstrated that SpAD enables sensitive, large-scale screening of drug responses and CRISPR gene knockouts without labeling - allowing unbiased morphological phenotyping at a new scale.
Years in the making - A great work (fine art) executed by our postdoc
@DicksonSiu1 and Alan Wong from
@hkusbms @hkumed
๐ Preprint:
biorxiv.org/content/10.1101/โฆ
#LabelFree #CRISPR #Drugscreen #QPI #Biophysics #SpAD #microscopy #omics