🌾 Hormone-free rice regeneration multiplex editing of previously "uneditable" AT-rich loci — in one system. The Plant Journal: iSpyMacCas9-Combo activates OsBBM1 (12×) via CRISPR-Combo, enabling 42% hormone-free regeneration and dramatically boosting editing at difficult targets. ✅ OsFLO6 (low-efficiency locus): finally editable ✅ 15/19 T0 plants: dual mutants; 6/19: triple mutants ✅ NAAR PAM — perfect for AT-rich promoter regions ❌ Cas12b-Combo: weak activation (~3×), failed to regenerate Qi lab | The Plant Journal doi.org/10.1111/tpj.70980 #CRISPR #Rice #CRISPRCombo #PlantBiology #MultiplexEditing

🌾 29 genes edited simultaneously in a single rice plant. No Cas12a. Just TREX2-SpCas9 optimized tRNA arrays. New in Plant Communications: TREX2 fusion boosts SpCas9 at tough loci (0% → 9.38%), and 38 rice tRNAs were screened to find 13 high-efficiency elements — two hit 100%. ✅ 83% of T0 plants: 19–27 OsCPK genes edited simultaneously ✅ Two plants: all 29 target genes knocked out ✅ Works in N. benthamiana too Zhou labs, CAAS/ZJU | Plant Communications doi.org/10.1016/j.xplc.2026.… #CRISPR #MultiplexEditing #Rice #PlantBiology #SpCas9

Summer break is over, but your CellEDIT-ed cells came back healthy, happy, and multiplexed — just the way you wanted. 🧬✨ While some cells return from vacation with nothing more than a sketchy nose job 👃😬 #CellEDIT delivers the full makeover: Vector-free, intra-nuclear delivery with precise RNP stoichiometry and multiplex genome editing — for results that are accurate, safe, and seriously impressive. 💅🧬 #CellGlowUp #BackToSchool #MultiplexEditing #SingleCellEngineering #PrecisionCRISPR #NextGenGeneEditing #CellEngineering #CustomCellLines #CellLineDevelopment #CellManipulation #GeneticallyEngineeredCells #SyntheticBiology