Why does multiplex IF get harder when biology gets more interesting? After 1–2 markers, visual review is not enough. This is not just more staining. It is a different data problem, and image analysis is what makes the biology usable. Full episode 👇. #MultiplexIF

Don’t miss poster #4920 this morning! @MariiaDenisovna will show how #multiplexIF of human pancreatic 3D CAF/ECM units, using @lunaphore @biotechne COMET & HORIZON, assigns pro- vs anti-tumor functional values. @FCCCGreenberg @FoxChaseCancer @katztemplemed #AACR26 @AACR

Multiplex IF gets harder when the biology gets more interesting. After 1–2 markers, it’s no longer about visual review. It’s about image analysis, complexity, and biological meaning. Full episode in first comment. #DigitalPathology #MultiplexIF #trlblzr

Proud and grateful to see this work published: jitc.bmj.com/content/13/12/e… What started in 2018 at @theNCI as an idea I kept returning to—how do we create a shared, reproducible language for increasingly complex immunohistochemistry and immunofluorescence assays?—has grown into a truly global, multi-specialty, multi-institutional collaboration. This initiative was initially supported by @abcam, then brought to the @sitcancer, where it evolved into a broad effort spanning academia, pharma, and industry, supported by the SITC Pathology Task Force and strongly advocated for by #JEDIs. None of this would have been possible without the exceptional leadership and coordination of the SITC staff, and the commitment of every contributor involved. I am deeply honored to have worked alongside this extraordinary group of co-authors: Carlo Bifulco @CarloBiful63522, Jaime Rodriguez, M.D., poh sheng JOE YEONG @JoeYeong, Guray A. Akturk, Michael Angelo, Carmen Ballesteros-Merino, PhD, Peter Bankhead @petebankhead, Subham Basu, Jorge Blando, Saska Brajkovic, Marco Cassano, Benjamin J. Chen, Ahmet F Coskun, Tricia R. Cottrell, Carlos E. De Andrea, Robin Edwards, Colt Egelston, Logan Engle, Marc S. Ernstoff, Rong Fan, Michael Feldman, Bernard A. Fox PhD @BernardAFox, Jerome Galon, Robyn Gartrell, Sacha Gnjatic, Benjamin Green, James Gulley MD PhD FACP @gulleyj1, Anne Hellebust, Stephen Hewitt, Travis J. Hollmann, MD, PhD, Lucas A. Horn, William J. Howat, Clifford Hoyt, Shawn Jensen, Arutha Kulasinghe, Wiem Lassoued, Steven Lott, PhD, MB(ASCP)QIHC, James R. Mansfield, Sebastian Marwitz, George J. Netto MD, David Page, Edwin Roger Parra Cuentas, David Rimm, Scott Rodig MD, PhD, Roberto Salgado, Denis Schapiro, Kurt Schalper, Joel C. Sunshin, Michael Surace, Ph.D., Alex Szalay, Magdalena Thurin, Jose C. Villasboas, Keith Wharton, Jr., MD PhD, Ignacio I Wistuba, Jennifer H. Yearley, Yinyin Yuan, George Zaki, James Ziai, and Janis M. Taube. The purpose of this work was to draw a roadmap for advanced IHC and immunofluorescence assays—particularly multiplex IF—to help standardize reporting, improve reproducibility, and establish a common scientific language in a rapidly evolving field. Standardization is not about restriction; it is about clarity, comparability, and trust. Ultimately, this effort is about better science, better translation, and—most importantly—better outcomes for the patients these tests are meant to serve. Grateful to everyone who contributed their expertise, time, and belief in this vision. This is how progress happens—together. #SITC #Immunotherapy #Pathology #MultiplexIF #IHC #CancerResearch #TranslationalScience #Collaboration #Standards @JohnsHopkins @

Streamline custom panel development on Lunaphore’s COMET™ platform with Bio-Techne’s growing catalog of antibodies, specifically validated for multiplex IF (mIF) on COMET™! Larger catalog for your unique experiments. bit.ly/3AbRd9p #MultiplexIF #COMET

Live from #IO360Summit! 🎬 Thank you to everyone who attended our Orion Technology Showcase this morning. 👋 Stop by Booth #38 to chat with our team and see Orion interactive data sets! #SpatialBiology #SpatialProteomics #MultiplexIF #RareCyte

Bio-Techne’s latest addition of rigorously tested antibodies for multiplex immunofluorescence (mIF) on Lunaphore COMET™. Browse our portfolio for a wide range of options to meet your research needs. bit.ly/3PHBeUG #MultiplexIF #MultiplexImmunofluorescence #COMET

GREAT DEBATE: Pre-treatment #predictive #biomarkers: INF-y vs. #Immunoscore vs. #multiplexIF /next-generation #spatialbiology. A 3-way debate.. Drs. Blank vs Galon vs Taube Dr.Janis M Taube wins.. 💥DETAILS ON BSKY @BernardAFox.bsky.social #Bridge2024 @sitcancer @PAscierto

Cool talk @PoojaVaid241 !! Look forward to the outcomes of you studY! #BreastCancer #triplenegative #multiplexIF

The new release of our Highplex FL module can generate interactive markup images, empowering investigation of biomarker colocalization and combined phenotypes. Click for more info! indicalab.com/news/halo-halo… #digitalpathology #pathtwitter #multiplexIF #HALOimageanalysis #IndicaLabs

TriCon is nearly here! Visit Dr. Jeff Dzubay's poster during afternoon refreshments to learn about automated #HALOai analysis of hyperplex images as well as the work of the #IndicaLabs Pharma Services team! #digitalpathology #pathtwitter #multiplexIF #HALOimageanalysis

The DPC: Europe afternoon poster session is starting soon! Visit Dr. Ines Nearchou's poster to see how #HALOai analysis of mIF images, collected by @lunaphore COMET, can offer insight into the TME. #digitalpathology #pathtwitter #spatialbiology #multiplexIF #HALOimageanalysis

This week is the #DigitalPathology & #AI Congress! 💻John Connelly will talk about using #ComputationalPathology and #ML to predict genomic features from histology images 🔬@MRC_TU Histopathology Facility will present their validation of a #multiplexIF assay for neural proteins

We have some exciting news coming to #SITC22! Stay tuned! #immunotherapy #spatialbiology #multiplexIF #precisionmedicine #immunooncology

#histowiz #Louisiana #sales #wednesdaythought #WednesdayMotivation #Wednesdayvibe #digitalpathology #AI #Histology #IHC #telepathology #multiplexIF #pathologyconsultation #imageanalyses

Dr. Ghislaine Lioux's @lunaphore Spatial Biology Week webinar on characterizing the cellular architecture of the TME using HALO and HALO AI is starting soon! link.lunaphore.com/tej #digitalpathology #pathtwitter #spatialbiology #multiplexIF #HALOimageanalysis #HALOai

Don't miss next Thursday's webinar on characterizing the cellular architecture of the TME using HALO and HALO AI during @Lunaphore Spatial Biology Week! link.lunaphore.com/tej #digitalpathology #pathtwitter #spatialbiology #multiplexIF #HALOimageanalysis #HALOai

Here, we used multiple and novel complementary technologies #MultiplexIF #spatialprofiling #CyTOF #geneexpressionprofiling to investigate the immune environment surrounding chemotherapy resistant high risk tumours and how chemotherapy can alter functions of several immune cells.

Don’t miss Dr. Ghislaine Lioux’s Oct. 6 webinar on characterizing the cellular architecture of the TME using HALO and HALO AI during @Lunaphore Spatial Biology Week! lunaphore.com/our-events/the… #digitalpathology #pathtwitter #spatialbiology #multiplexIF #HALOimageanalysis #HALOai

Our new paper, “Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging” is now online at Nature Communications Biology! #MISILab #spatialbiology #Multipleximaging #multiplexIF @WCMCPathology nature.com/articles/s42003-0…