ALT Classification of root systems of three graminoid species in three tested configurations. A CONF1 at an aperture of f/5.6 with the following acquisition settings: TDI 3, Pixel blur 2.5, Binning 2 × 2. Two biological replicates for each species were employed. B CONF2 with the acquisition settings of TDI 1, Pixel blur 0, Binning 1 × 1, unless otherwise indicated. One biological replicate for each species was imaged under the different acquisition settings. C CONF3 with the following acquisition settings: TDI 1, pixel blur 0, binning 1 × 1. One biological replicate for each species was imaged at the different magnifications. In all configurations, the RF model was trained using SAM classifications. A. odor. = A. odoratum, D. flex. = D. flexuosa, E. vagi. = E. vaginatum