#SpatialTranscriptomics #Atherosclerosis CosMx 6k @brukerspatial over 1119 fields of view of 4 patients (♀️♂️ stable/unstable plaques)➡️ Sex-/Stability-related lesional cell architecture😎 scRNAseq Fine subtyping of #SmoothMuscleCell, T lymphocyte & macrophage in sex-related plaque stability Harmony➡️ batch correction Fast Nearest Neighbor R (50 μm radii)➡️Spatial neighborhood AtoMx still requires further optimization for vasculature...🧐 @jae_hbyun @atvbahajournals 2026 ahajournals.org/doi/10.1161/…

#Ferroptosis #VascularCalcification SREBP1 transcriptionally⏫iASPP (likely FASN▶️FAO, BHLHE40▶️Circadian, too)➡️ ⏬#SmoothMuscleCell #Ferroptosis #Osteogenesis (BMP2 RUNX2 Calcification) Anti-calcific 1⃣Liproxstatin-1 (Ferroptosis inhibitor) in vitro 2⃣SREBP1 overexpression in vitro & in vivo Pro-calcific 1⃣Erastin in vitro 2⃣sh-iASPP in vitro #CardiovascDiabetol 2025 cardiab.biomedcentral.com/ar…

NAMPT #AbdominalAorticAneurysm 👤AAA ⏬NAMPT OXPHOS mitochondrial ETC #SmoothMuscleCell contractile phenotype ⏫Senescence of SMC non-SMC ⏫B cell #PlasmacytoidDendriticCell 1⃣#ExtracellularVesicle derived from NAMPT-overexpressing #hiPSC-#MesenchymalStemCell or 2⃣P7C3 a NAMPT chemical activator 👉restores SMC NAD 👉alleviates SMC senescence &🐭AAA MSC-EV per se benefits, too🤓 @BioactiveMat 2023 sciencedirect.com/science/ar…

miR-26b as a repressor #AorticMedialCalcification ⏬Aortic Fibroblast BMP4 to #SmoothMuscleCell SMAD1 (direct target gene of miR-26) signaling miR-26b KO drives spontaneous aortic calcification w/o disrupting elastin fiber drastic alteration in #EndothelialCell SMC Fibroblast states scRNAseq @caporalilab #CardiovascRes 2025 @ESC_Journals academic.oup.com/cardiovascr…

Loss of contractile phenotype (⏬SMMHC SMTN ⏫S100A4 MMP2) of #SmoothMuscleCell & Elastin fiber in #AcuteAorticDissection Type A/B? Any aneurysm?🧐 24 case (non-monogenic) vs 17 control "aortic wall adjacent to the entry site of the dissection" #AortaEd #CJCOpen 2025 @CJCJournals cjcopen.ca/article/S2589-790…

#SIRT6 #VascularCalcification Rat #HighFatDiet VitD3 Nicotine ▶️Aortic medial calcification & ⏬SIRT6 SIRT6 overexpression ⏬Palmitic acid-induced #SmoothMuscleCell Apoptosis & Osteogenesis #JHyperten 2023 journals.lww.com/jhypertensi…

#Autophagy #VascularAging SM22α #SmoothMuscleCell Atg7 KO🐭3.5 mo Aorta myography with oscillatory tension 10 Hz 👉Peterson modulus⏫ pressure-dependent systolic 80~300 mmHg diastolic 40~260 mmHg ↔️🫀functions Paul Fransen lab #PflügersArchiv 2020 link.springer.com/article/10…

Transcript signature of lineage-specific mouse proximal thoracic aortic #SmoothMuscleCell Wnt1-Cre cardiac neural crest (inner media)-derived SMC Ccn5 Ccdc42 Tes Eln Aebp1 Galnt6 Ccn2 Aopep Wtip Mef2c-Cre second heart field (outer media)-derived SMC Des Tnnt2 Hand2os1 Psd Gpc3 Meis2 Dcn Gm34030 Palld Nrtn Lum Cfh #AortaEd David Dichek lab @atvbahajournals 2025 ahajournals.org/doi/10.1161/…

1400W, an iNOS inhibitor, reduces #ThoracicAorticAneurysm #ThroacicAorticDissecion in BAPN mouse, in addition to previously reported Marfan mice A possible role in #SmoothMuscleCell Mitochondrial fission/fusion #EurJPharm 2025 sciencedirect.com/science/ar…

#VascularCalcification 🐭aortic #SmoothMuscleCell in anchored 3D fibrin hydrogel for 14 d 👉Accumulate proteoglycan (IHC Proteomics) 👉No elastin fiber formation 👉TGFβ-responsive 👉Calcifiable, retaining similar individual sensitivity to 2D culture sciencedirect.com/science/ar…

#SmoothMuscleCell #Aging Mechanobiology 24- vs 4-mo Rat soleus muscle feed artery ⏬Myogenic tone (so flat 45~135 cmH2O! Learn new knowledge😋)▶️rescued by 1h 130 cmH2O pretreatment Cultured Aged SMC 👉Bulk RNAseq ⏬SMC contractile markers #FocalAdhesion 👉Faster kinetics (⏫SM α-actin filamentation ⏫vinculin clustering) to biaxial #CyclicStretch 👉Inert to Pressure➡️⏬SM α-actin filament on soft 4 kPa matrix 👉Sensitized to Pressure➡️⏫SM α-actin filament ⏫integrin β1 clustering on stiff 100 kPa matrix Andreea Trache lab @FrontPhysiol 2025 frontiersin.org/journals/phy…

#VeinAging #VaricoseVein-associated great saphenous venous medial wall remodeling across adulthood 18~89 y/o Normal Vein 👉Subintimal longitudinal #SmoothMuscleCell bundle peak at middle age 👉Outer medial longitudinal SMC bundle ⬆️with Age Varicose Vein 👉Alternating zones of hypertrophic & atrophic (electron-dense on TEM) SMCs 👉Excess collagen deposit b/w SMCs 👉⬇️subintimal longitudinal SMCs in elderly (vs normal) 👉⬆️outer medial longitudinal SMC in young & middle (vs normal) @JCM_MDPI 2025 mdpi.com/2077-0383/14/18/644…

#HutchinsonGilfordProgeriaSyndrome #SciArt SM22α-Cre #SmoothMuscleCell-specific Progerin transgene (Lmna LCS/LCS) drives #EndMT #Atherosclerosis in 🐭 high fat diet ⏫Aortic Endothelial Cell permeability (Atto565-LDL i.v.) ⏫Leukocyte adhesion/extravasation ⏫Lesional EC infiltr▶️An intriguing ColIV-rich plaque😮 ⏫Intimal Snail, Zeb2; ⏬intimal Twist1, Slug, Zeb1 ⏫Aortic TGFβ1 (not TGFβ2), contributed by intraplaque mesenchymalized EC What is the paracrine signal of progeroid aortic SMCs?😆 @CircAHA 2024 ahajournals.org/doi/10.1161/…

The quantitative analysis of Re-orientation of #SmoothMuscleCell #CollagenFiber in🐭#Atherosclerosis #Calcification Annexin A2/5/6 (#MatrixVesicle markers)⬆️ only seen in zones of SMC/Collagen disorganization Xufeng Niu & Yubo Fan labs Research 2025 spj.science.org/doi/10.34133…

Gelsolin as a selective #Pericyte marker vs #SmoothMuscleCell Fibroblast in mouse #SkeletalMuscle Antibody R&D MAB8170 Cryosection BulkRNAseq (SkM PDGFRβ SMA− pericyte vs PDGFRβ SMA SMC) vs (Brain pericyte vs SMC) #SciRep 2025 nature.com/articles/s41598-0…

PRMT5 di-methylates H3R8 & H4R3▶️ ⏬Myocardin-dependent #SmoothMuscleCell contractile gene transcription ⏫SMC proliferation/migration & intimal hyperplasia in injured🐭carotid artery PRMT5 (& PRMT4) ⬆️in PDGF-BB-treated SMC & 👤coronary #Atherosclerosis Evidence from SMC PRMT5 KO🐭 Lentiviral shPRMT5 EPZ015666 PRMT5i #CardiovascRes 2023 academic.oup.com/cardiovascr…

PRMT5 methylates & stabilizes KLF4 protein▶️ ⏫#SmoothMuscleCell proliferation/migration & intimal hyperplasia in injured🐭carotid artery Evidence from #SmoothMuscleCell PRMT5 KO🐭 & Lentiviral PRMT5 PRMT5⬆️ in👤carotid stenosis & PDGF-BB-treated SMC #CardioVascRes 2023 @ESC_Journals academic.oup.com/cardiovascr…

PRMT5 methylates NF-κB p65 to enhance trimethylamine N-oxide-NOX4-induced #SmoothMuscleCell inflammation SM22α SMC PRMT5 KO🐭 carotid wire injury TMAO i.p.▶️ ⏬p65 methylation ⏬Vascular cell VCAM1 ⏬CD68 recruitment #CellDeathDis 2022 @cddpress nature.com/articles/s41419-0…

#SmoothMuscleCell m6A Epitranscriptomics METTL14 ⬆️in 🐭#ThoracicAorticAneurysm (AngII HFD) or erastin-treated SMC Liproxstatin-1 or AAV-shMettl14 rescues 🐭TAA METTL14-IGF2BP2 stabilizes ACSL4 mRNA➡️ SMC #Ferroptosis SRAMP/deepSRAMP: m6A site prediction @AJPCellPhys 2025 journals.physiology.org/doi/…

#SmoothMuscleCell Mettl14, ⬆️in injured artery, aggravates #IntimalHyperplasia via ⏫nascent KLF4 & SERPINE1 mRNA synthesis (Not impacting mRNA stability) Likely Mettl3, but not other m6A readers/writers/erasers, plays a minor role @JCI_insight 2025 insight.jci.org/articles/vie…