15 Apr 2024
We’re switching it up! Celebrate EAL’s new #MethodMonday by dancing around to your favorite songs! #loveyourbrain #EAA 💃🪩🕺
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25
26 Feb 2024
#MethodMonday 49: GRADE in systematic reviews with meta-analyses and clinical practice guidelines
1) doi.org/10.1111/aas.14386
2) doi.org/10.1111/aas.14384
This week includes two papers on using the GRADE (@GRADE_WG) approach in SRMAs (#1) and clinical practice guidelines (#2) 1/...
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2,684
29 Jan 2024
#MethodMonday 48: Estimating means/SDs from medians/IQRs/ranges?
1) doi.org/10.1186/1471-2288-14…
2) training.cochrane.org/handbo…, sections 6.5.2.9 10.5.3, 6.5.2.4-6
This week covers methods for estimating means and SDs from medians/IQRs/ranges, as frequently done in meta-analyses. 1/..
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25
3,674
22 Jan 2024
#MethodMonday 47: Factorial trials
doi.org/10.1186/1471-2288-3-…
doi.org/10.1001/jama.2023.19…
This week focuses on factorial trials, where participants are randomised to multiple 'sets' of interventions (factors), i.e. A vs B and C vs D, leading to combinations A C, A D, B C, and B D 1/..
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2,784
8 Jan 2024
#MethodMonday 46: Analysing outcomes also measured at baseline in trials
doi.org/10.1136/bmj.323.7321…
This week's paper by @VickersBiostats and Altman discusses how outcomes measured both at follow-up but also at baseline in trials should be analysed 1/..
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6,122
18 Dec 2023
#MethodMonday 45: 10 simple rules for good research practice
doi.org/10.1371/journal.pcbi…
This article follows #MethodMonday 44, and includes a checklist of 10 points covering the essentials for good research practice 1/..
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13
1,571
4 Dec 2023
#MethodMonday 44: Researcher degrees of freedom and variation in analyses/results
doi.org/10.3389/fpsyg.2016.0…
doi.org/10.1177/251524591774…
Two papers from psychology, but the central points apply to most fields 1/..
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681
27 Nov 2023
#MethodMonday 43: Directed acyclic graphs (DAGs)
doi.org/10.1001/jama.2022.18…
This week's paper is a brief introduction to DAGs. DAGs are used as a tool to specify analytical models in e.g. observational studies and a formalised approach to variable selection.. 1/..
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36
122
19,552
20 Nov 2023
#MethodMonday 42: Immortal time bias
catalogofbias.org/biases/imm…
doi.org/10.1002/pds.1357
This week covers immortal time bias in observational studies - periods where participants in a cohort by definition cannot experience the event of interest. 1/...
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22
4,644
13 Nov 2023
#MethodMonday 41: Environmental life cycle assessments
doi.org/10.1097/ogx.00000000…
This week's paper suggested by @TineMeyhoff - thanks! Suggestions always welcome.
It covers life cycle assessments (LCAs), a method to quantify the enviromental impacts of medical practice 1/..
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710
6 Nov 2023
#MethodMonday 40: Prospective meta-analyses
doi.org/10.1136/bmj.l5342
This week's article concerns prospective meta-analyses (PMAs) - meta-analyses planned before the included studies are completed. It how PMAs differ from conventional systematic reviews with MAs, ... 1/...
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10
3,324
30 Oct 2023
#MethodMonday #39: Cluster-randomised trials
doi.org/10.1093/ije/dyad064
#MethodMonday is back after a break, this time with a paper on cluster RCTs.
The article covers key methodological considerations, including the potential disadvantages of cluster trials 1/..
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25
4,696
9 Oct 2023
👨🔬This #MethodMonday Lautaro Gándara and colleagues at the University of Buenos Aires reveal how to design FRET-based sensor experiments👩🔬 Read the full #OpenAccess🔓Methods & Techniques article here: journals.biologists.com/bio/…
ALT Metabolic FRET-based sensors. Illustration of the functioning of the lactate sensor Laconic (San Martín et al., 2013). Scheme of the molecular mechanism of the sensor.
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1,132
2 Oct 2023
🧬🔬This #MethodMonday Katie M. Toperzer and colleagues @FloridaTech use a CaFE reporter, a calcium indicator which aids the visualisation of sperm-egg fusion during fertilisation. 🔓 Read the full #OpenAccess Research Article here: journals.biologists.com/bio/…
ALT CaFE fluorescence is dependent upon functional sperm - Time-lapse images of ovulation.
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849
18 Sep 2023
🔬This #MethodMonday @StanStrawbridge and colleagues describe a new mathematical framework classifying interior and exterior points of imaged cells within the early mouse embryo. Read the full #OpenAccess Methods and Techniques article here: journals.biologists.com/bio/…
ALT Morphogenetic events during pre-implantation mouse development.
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2,078
18 Sep 2023
#MethodMonday 38: Common statistical misinterpretations
doi.org/10.1007/s10654-016-0…
This paper by @Lester_Domes @stephensenn covers some of the most common misinterpretations of statistical tests, p-values, confidence intervals and power. 1/..
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12,266
11 Sep 2023
#MethodMonday 37: Bayesian analyses
doi.org/10.2196/10873
doi.org/10.1136/bmjopen-2018…
These papers provide an introduction to Bayesian statistical methods and a comparison with conventional frequentist methods (incl. p-values, confidence intervals, null hypothesis tests) 1/..
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51
12,141
4 Sep 2023
This #MethodMonday Tomohiro Tamari and colleagues
@jacksonlab demonstrate that the zygote electroporation method is effective for generating knockout mice in multiple inbred strains. Read the full #OpenAccess Methods and Techniques article here: journals.biologists.com/bio/…
ALT Genome editing and genotyping design on Hr gene. Orange arrows (gRNA-1 and gRNA-2) represent the gRNA target sites flanking exon 3. Blue arrows represent PCR primers, including the size of the PCR amplicon for G0 genotyping. Allele A represents the wild-type allele; allele B represents the exon deletion allele. gRNA, guide RNA.
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938
4 Sep 2023
#MethodMonday 36: Random forests
nature.com/articles/nmeth.44…
This paper covers ensemble methods, specifically bagging and random forests. In brief, these methods rely on using a simple regression tree to split the dataset... (1/...)
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14
2,315
28 Aug 2023
#MethodMonday 35: Clustering
doi.org/10.1038/nmeth.4299
Clustering methods may be used to find clusters of similar patients based on multiple variables. This paper introduces general principles, some specific methods, assessment of similarity within clusters (ideally high).. (1/..)
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3,063