@megatev profile picture
David Edgell @megatev

Genome editing, synthetic biology and mobile genetic elements.

Joined June 2016
  • Tweets 293
  • Following 122
  • Followers 420
50 Photos and videos
Paper alert! 📢 PHYCUT: multiplexed Cas9 editing for microalgae. pubs.acs.org/doi/10.1021/acs… Amazing work from #EmilyStuckless with assist from #Gloor_lab. Multiplexed knockouts of fucosyltransferase genes for humanization of N-linked glycosylation pathway.

PHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the Diatom Phaeodactylum...

Diatoms are globally significant microalgae that contribute ∼20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom Phaeodactylum tricornutum has emerged as a promis...

pubs.acs.org
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🔊Paper alert! Incredible work from #EmilyStuckless and fellow Edgell lab members on PHYCUT multiplex Cas9 editing system for Phaeodactylum tricornutum. biorxiv.org/content/10.1101/…

PHYCUT: Scalable multiplex CRISPR/Cas9 editing for genome engineering in the diatom Phaeodactylum...

Diatoms are globally significant microalgae that contribute ∼ 20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom Phaeodactylum tricornutum has emerged as a...

biorxiv.org
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PHYCUT used for glycoengineering by knocking out fucosyltransferases as first step in humanizing N-linked glycosylation pathway!
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Thanks to @NSERC_CRSNG for funding #ggloor for collaboration @SchulichMedDent @westernuBiochem
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David Edgell@megatev
11 Sep 2025
Paper update! Now online pubs.acs.org/doi/10.1021/acs… at ACS Synthetic Biology @ACSPublications

Genetic Entanglement Enables Ultrastable Biocontainment in the Mammalian Gut

Imbalances in the mammalian gut are associated with acute and chronic conditions, and using engineered probiotic strains to deliver synthetic constructs to treat them is a promising strategy....

pubs.acs.org
David Edgell@megatev
13 Aug 2025
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…
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David Edgell retweeted
David Edgell@megatev
13 Aug 2025
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…

Genetic entanglement enables ultra-stable biocontainment in the mammalian gut

Imbalances in the mammalian gut are associated with acute and chronic conditions, and using engineered probiotic strains to deliver synthetic constructs to treat them is a promising strategy....

biorxiv.org
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David Edgell@megatev
14 Aug 2025
Paper 🚨📢! Adenine methylation in PAM sequence regulates Staph aureus Cas9 activity in bacteria. biorxiv.org/content/10.1101/…. Superstar collab between #DaltonHam @nortnlentivirus

PAM adenine methylation and flanking sequence regulate SaCas9 activity in bacteria

Cas9 nucleases are the effectors of the class 2 type II CRISPR system in bacteria and function to restrict invading DNA. They can also be used with single guide RNAs (sgRNAs) as antimicrobials and...

biorxiv.org
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David Edgell@megatev
14 Aug 2025
crisprHAL ML model found poor activity at PAM sites with GATC sequences....seem familiar? GATC is DNA adenine methyltransferase site in bacteria. Large scale in vivo assays, Nanopore sequencing, and in vitro assays all show that G*ATC methylation impacts SaCas9 activity.
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David Edgell@megatev
14 Aug 2025
All the details in the paper! Another stellar collaboration with #GloorLab. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent
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David Edgell@megatev
13 Aug 2025
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…

Genetic entanglement enables ultra-stable biocontainment in the mammalian gut

Imbalances in the mammalian gut are associated with acute and chronic conditions, and using engineered probiotic strains to deliver synthetic constructs to treat them is a promising strategy....

biorxiv.org
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David Edgell@megatev
13 Aug 2025
Amazing work by @gary_wfoo with big assist from @deheinrichs. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent
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David Edgell@megatev
16 May 2025
Incredibly lucky to have played a small role in this amazing paper. nature.com/articles/s41586-0…. Congrats to all involved!! @SchildPoulter @SchulichMedDent @WesternU #Zhalaboratory @Columbia

Ku limits RNA-induced innate immunity to allow Alu expansion in primates

Nature - Studies on the essentiality of Ku in human cells reveal that Ku interacts with diverse double-stranded RNA molecules, including antisense Alu, and enables tolerance of Alu sequence...

nature.com
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David Edgell@megatev
27 Jun 2024
Congrats to trainees @THamilton26 @brjoris @nortnlentivirus and collaborators @BogumilKaras @gbgloor @RodrigueLab for their paper being selected as one of the Top 25 ACS papers for World Micrbiome Day! pubs.acs.org/page/vi/world-m…

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David Edgell retweeted
Canadian Journal of Microbiology@CanJMicrobio
22 May 2024
📣 @CanJMicrobio is accepting papers for a collection showcasing Canadian research in #Synthetic_Biology. 🧬More info: ow.ly/wYGx50RKYtK @CSM_SCM @DavidKwanPhD @BogumilKaras @SynBioCanada @megatev ✨New extended due date! 📅 November 15, 2024
Call for Papers - Canadian Journal of Microbiology

ALT Call for Papers - Canadian Journal of Microbiology
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David Edgell@megatev
10 Apr 2024
📢New gene editing technology biorxiv.org/content/10.1101/…. This is result of amazing collaboration with @SpecificBio and supported by @NSERC_CRSNG @MitacsCanada @LumiraVentures @adMare_Bio @westernuBiochem @SchulichMedDent 🧵👇

Harnessing RNA-based DNA repair pathways for targeted gene editing

Recent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that the asymmetric DNA overhangs generated by the small TevSaCas9 dual nuclease informs a simple...

biorxiv.org
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David Edgell@megatev
10 Apr 2024
4/n Guess what? Size does matter - everything fits within a single AAV vector (TevSaCas9 repair guide). NO need for co-delivery of exogenous repair factors, accessory repair proteins/templates, or small/molecules/proteins to modify repair pathways.
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David Edgell@megatev
10 Apr 2024
5/n Early stages yet but excited about potential for rep-editing. Stay tuned for updates.
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David Edgell retweeted
Schulich WesternU@SchulichMedDent
13 Mar 2024
Projects by #SchulichMedDent researchers have received a combined $2 million in project grant funding from @CIHR_IRSC. One project, led by David Edgell (@megatev), aims to develop effective methods for eliminating harmful bacteria. Get the details ⤵️ brnw.ch/21wHQZc

Targeting toxic bacteria among research projects to receive CIHR project funding

CIHR project grants will help Schulich Medicine & Dentistry researchers, like David Edgell and Dean Betts, create news answers to our health challenges.

schulich.uwo.ca
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