Neuroscience @ Western U Studying Stress with Patch Clamp Electrophysiology. Inoue Lab. He/Him. Former Carleton U Raven!

Joined July 2020
12 Photos and videos
Sam Mestern retweeted
‼️ Only 10 days left until abstract submission and regular registration deadline for the Southern Ontario Neuroscience Association (SONA) 🧠 meeting at @McMasterU on May 2, 2026! We have a fantastic lineup of speakers - see flyer! Registration and abstract links are also below 👇
1
1
6
239
Sam Mestern retweeted
📅 SAVE THE DATE 📅 The 44th annual meeting of the Southern Ontario Neuroscience Association will be held on Saturday May 2, 2026 at @McMasterU in Hamilton, Ontario. Abstract submission and registration will open in 2 days! More info sonasfn.org/meetings/ Please share🔄

3
8
493
Sam Mestern retweeted
LLM AI assistants will always be at risk of hallucinating/sycophancy/lying. Can they still be useful for accelerating good research? Yes! But we need a *lot* of guardrails. If you've wanted to learn how to use tools like Claude Code to *responsibly* accelerate quantitative research, I'm running a webinar with @aefpweb next week that's free and open to the public to introduce folks to DAAF: the Data Analyst Augmentation Framework. In short: DAAF is an open-source (read: forever free!) instruction layer that sits on top of Claude Code that helps it think and operate more like a rigorous scientist that you can collaborate with for *any* data analysis task. It prioritizes auditability/reproducibility (verify, don't trust!), keeps you in the driver's seat at all times, and handles any data task with rigor *and* speed: documentation lookups, data cleaning, complex joins, regression analyses and causal inference (new in DAAF v2.0.0 coming next week!!), data dashboarding, the works. Think of it like an exo-skeleton for skilled researchers to *scale* their expertise and impact, rather than replace/automate it. Registration link in the first comment below! Note the framing is for an education research audience, but DAAF is immediately applicable to *any* data analysis in *any* field. This webinar with AEFP kicks off a wild month of DAAF-related workshops and seminars with fantastic orgs like Gates Foundation, Northwestern, Georgetown, UVA, SREE, and more. Stay tuned for more free recorded educational resources on that front. And be on the lookout for DAAF v2.0.0 updates early next week. Huge updates and expansions to be VERY excited about; Claude's best summary of what to expect attached :)
2
4
21
2,591
Sam Mestern retweeted
Where in the brain does DANDI have data? Now you can find out. DANDI Atlas maps datasets onto the Allen CCF mouse brain atlas in interactive 3D. Explore 47 dandisets across 353 brain regions. atlas.dandiarchive.org Announcement: about.dandiarchive.org/blog/… youtube.com/watch?v=D8514CLV…

2
6
694
Sam Mestern retweeted
Today, I’m launching DAAF, the Data Analyst Augmentation Framework: an open-source, extensible workflow for Claude Code that allows skilled researchers to rapidly scale their expertise and accelerate data analysis by as much as 5-10x -- without sacrificing the transparency, rigor, or reproducibility demanded by our core scientific principles. You (yes, YOU!) can install and begin using it in as little as 10 minutes from a fresh computer with a high-usage Anthropic account (crucial accessibility caveat, unfortunately very expensive!) github.com/DAAF-Contribution… DAAF explicitly embraces the fact that LLM-based research assistants will never be perfect and can never be trusted as a matter of course. But by providing strict guardrails, enforcing best practices, and ensuring the highest levels of auditability possible, DAAF ensures that LLM research assistants can still be immensely valuable for critically-minded researchers capable of verifying and reviewing their work. In energetic and vocal opposition to deeply misguided attempts to replace human researchers, DAAF is intended to be a force-multiplying "exo-skeleton" for human researchers (i.e., firmly keeping humans-in-the-loop). The base framework comes ready out-of-the-box to analyze any or all of the 40 foundational public education datasets available via the Urban Institute Education Data Portal (educationdata.urban.org/docu…), and is readily extensible to new data domains and methodologies with a suite of built-in tools to ingest new data sources and craft new Skill files at will! With DAAF, you can go from a research question to a shockingly nuanced research report with sections for key findings, data/methodology, and limitations, as well as bespoke data visualizations, with only five minutes of active engagement time, plus the necessary time to fully review and audit the results (see my 10-minute video demo here: youtu.be/ZAM9OA0AlUs). To that crucial end of facilitating expert human validation, all projects come complete with a fully reproducible, documented analytic code pipeline and consolidated analytic notebooks for exploration. Then: request revisions, rethink measures, conduct new sub-analyses, run robustness checks, and even add additional deliverables like interactive dashboards, policymaker-focused briefs, and more -- all with just a quick ask to Claude. And all of this can be done *in parallel* with multiple projects simultaneously. By open-sourcing DAAF under the GNU LGPLv3 license as a forever-free and open and extensible framework, I hope to provide a foundational resource that the entire community of researchers and data scientists can use, benefit from, learn from, and extend via critical conversations and collaboration together. By pairing DAAF with an intensive array of educational materials, tutorials, blog deep-dives, and videos via project documentation and the DAAF Field Guide Substack (daafguide.substack.com/ – MUCH more to come!), I also hope to rapidly accelerate the readiness of the scientific community to genuinely and critically engage with AI disruption and transformation writ large. I don't want to oversell it: DAAF is far from perfect (much more on that below!). But it is already extremely useful, and my intention is that this is the worst that DAAF will ever be from now on given the rapid pace of AI progress and (hopefully) community contributions from here. More than anything, I just hope all of this work can somehow be useful for my many peers and colleagues trying to "catch up" to this rapidly developing (and extremely scary) frontier. It's a wild time, but we need as many people informed and at the table together as possible. Never used Claude Code? No idea where you'd even start? My full installation guide walks you through every step -- but hopefully this video shows how quick a full DAAF installation can be from start-to-finish. Just 3mins! youtube.com/watch?v=jqkVLXA1… Learn more about my vision for DAAF, what makes DAAF different from other attempts to create LLM research assistants, what DAAF currently can and cannot do as of today, how you can get involved, and how you can get started with DAAF yourself! github.com/DAAF-Contribution… So there it is. I am absolutely as surprised and concerned as you are, believe me. With all that in mind, I would *love* to hear what you think, what your questions are, what you’re seeing if you try testing it out, and absolutely every single critical thought you’re willing to share, so we can learn on this frontier together. Thanks for reading and engaging earnestly!
13
79
442
79,179
Sam Mestern retweeted
NWB just turned 10 years old! Researchers worldwide have downloaded 1.9 PB of NWB data from the @DANDIArchive. This animation shows the reach of NWB, facilitating collaboration across the globe. What impact has open neurophysiology data had on your science? Share your stories! 🧠
5
15
4,867
Sam Mestern retweeted
The Hypothalamus, a phylogenetically ancient brain region where action potentials and hormones interface to produce hybrid signalling cascades; ultimately enabling our responses to stress. Check Hiro's paper. Amazing work led by @NeuroStress_Lab. biorxiv.org/content/10.1101/…
6
20
1,032
Sam Mestern retweeted
Check out our new perspective paper on metastability in neuroscience published @PhysRevE doi.org/10.1103/PhysRevE.111…
1
5
274
Sam Mestern retweeted
Last week, I may have DEI’d too close to the sun and attracted anti-NIH folks to my profile who then claimed that my research is useless and a waste of time and money. I beg to differ! Allow me to explain in lay terms how my research in mice is relevant to human health: 🧵
My PhD work is out! How do mothers balance tending to their own needs, such as eating, and taking care of their offspring? We examined the link between feeding and parenting neural circuits and how they are modulated postpartum in mice 🧵 (1/9) PREPRINT: biorxiv.org/content/10.1101/…
15
64
870
119,703
Sam Mestern retweeted
🚨 Exciting news! @NeuroStress_Lab & @Timothy_Bussey have received @CIHR_IRSC funding to explore the role of the prefrontal cortex in behavioral selection under stress. This research could unlock new insights into how stress impacts decision-making! 🧠💡#Neuroscience #Stress
2
6
244
Sam Mestern retweeted
Excited to share my latest work with @DiedrichsenJorn and @andpru. In this work, we ask whether motor sequence learning is motoric at all! Check out the 🧵version of the abstract: biorxiv.org/content/10.1101/… 1/n

4
21
55
6,078
Sam Mestern retweeted
Evolution of the visual system has permeated the basic processing unit in the neocortex, the single neuron. A team effort ⁦@nxwm_network⁩. Single neuron diversity supports area functional specialization along the visual cortical pathways | bioRxiv biorxiv.org/content/10.1101/…
1
4
19
819
Sam Mestern retweeted
Congrats @smestern !! Awesome to see @NeuroCarleton alumni do great things!!
Congrats to @SMestern, Ph.D. student at @WesternU, for winning the MapMySpikes challenge! His tool, PatchOTDA, excelled in mapping electrophysiological data to molecular properties. 🧠🏆 More on his tool and the MapMySpikes challenge: alleninstitute.org/news/cana…
2
7
314
12 Dec 2024
I’m really excited to share this open-science side project I’ve been working on related to patch-clamp electrophysiology! If you’re into this or have some data you’d like to experiment with, I’d love for you to check it out. Big thanks to Allen Institute for the opportunity!
Congrats to @SMestern, Ph.D. student at @WesternU, for winning the MapMySpikes challenge! His tool, PatchOTDA, excelled in mapping electrophysiological data to molecular properties. 🧠🏆 More on his tool and the MapMySpikes challenge: alleninstitute.org/news/cana…
3
3
13
2,412
12 Dec 2024
If you have any questions, want to impletment it yourself, or just want to talk about cool patch-clamp data & open science feel free to contact me!
1
2
50
Sam Mestern retweeted
Congrats to @SMestern, Ph.D. student at @WesternU, for winning the MapMySpikes challenge! His tool, PatchOTDA, excelled in mapping electrophysiological data to molecular properties. 🧠🏆 More on his tool and the MapMySpikes challenge: alleninstitute.org/news/cana…
1
6
4,912
Sam Mestern retweeted
Winter ⁦@WesternU⁩ campus.
3
5
33
2,394
Sam Mestern retweeted
Our group @WesternU and @FieldsInstitute Lab for Network Science has postdoctoral and graduate research positions available. We are looking for scientists to bring together applied mathematics, neural data, and neural networks. Join our collaborative team!
2
13
27
3,954