In 216 ligand-bound structures of human FABP isoforms, many at better than 1.2 Å resolution, an estimated 15% of the ligands had a different chemical composition to that expected  #StructureBasedDrugDesign #FattyAcidBindingProteins #LigandBinding doi.org/10.1107/S20597983250…

We’re scaling structure as a core capability in AI-native drug design. #TeamGenerate is searching for an Executive Director of CryoEM to lead a multidisciplinary team operating within one of the most advanced high-throughput structural biology platforms in the world. Reporting to our Chief Technology Officer, you’ll set the vision and deliver on the execution—expanding what’s possible in structural throughput and data quality. You’ll partner closely with machine learning, automation, and protein science teams to embed #cryoEM into a true design–build–test–learn loop. This is an opportunity to shape a capability that will transform structural biology from a bottleneck into a high-speed engine for discovery—redefining how medicines are conceived, designed, and developed. If this sounds like your next challenge, let’s talk. generatebiomedicines.com/ope…

High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1 dlvr.it/TMMdLz

Four case studies are presented in which rationally designed surface modifications were key to establishing crystallization conditions for the target proteins @ActaCrystD @IUCr #SurfaceModification #SurfaceEntropyReduction #CrystalPacking doi.org/10.1107/S20597983240…

Our high-resolution (2.2 Å) in situ structural work reveals details of translational landscape, with various cofactors and antitumor drug/inhibitor, all directly within human cells! biorxiv.org/cgi/content/shor…

Structural characterization of two full length γδTCR/CD3 complexes | bioRxiv biorxiv.org/content/10.1101/…

New paper out in bioarxiv!. In collaboration with the team of Maks Ovsjanikov, Vincent Mallet developed an algorithm that is available as a plug-in for @UCSFChimeraX to find antibodies and nanobodies, within seconds, in #cryoem densities. biorxiv.org/content/10.1101/…. Try it out!

Check out our new work by fabulous grad student Rodrigo @TexasScience @ut_mbs! Guide RNA folds into gnarly geometry to tether tiny protein domains together. Fun collaboration with @metagenomi! DNA targeting by compact Cas9d and its resurrected ancestor biorxiv.org/content/10.1101/…

Update on our kinase domain side project (37 kDa with a ligand): Previous sample had severe preferred orientation issues, pausing the project. Now back at it with new 2D classification results. Still preferred orientation persists, but we're optimistic about getting a map. #TGIF

Time-resolved cryo-EM of G-protein activation by a GPCR | Nature disq.us/t/4nd6xsj

This fantastic story is out on structural analysis of NLRP3 inflammasome activation. Happy to be part of it! Please check it out! nature.com/articles/s41467-0…

Didn't think I would ever post ApoF, but here we go. First couple hours of test data collection on our new Tundra. How easy was that! And the best part: it's not hidden in a basement, but on the 3rd floor, one flight of stairs away from the office :D

#Cryoem #ai #drugdiscovery

Excited to share our preprint: “Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase” in which we obtained a structure of a 46-kDa membrane protein to 2.2 Å resolution without fiducials. tinyurl.com/zycuw3p6

My indispensable right hand Natalya Dudkina is hiring, join our exciting R&D group at Thermo Fisher Scientific as Application Engineer, Cryo-EM. RT please! jobs.thermofisher.com/GmABVU…

Our structure biology and protein design team embedded within the vibrant scientific environment of Large Molecules Research at Sanofi in Cambridge Crossing, MA has an exciting postdoc opportunity to accelerate the development of next generation biologics. #postdocjobs #postdoc

Please check our posters at #IMC20 today (9/13, Wed.) : Cryo-EM structure determination of sub-50-kDa protein : a case study on maltose binding protein - Kunwoong Park (LS 05.P, #2087). See you at the poster session.

As promised, I've got our #IMC20 poster ready to go! If you're at the conference, swing by and say hi. Got questions? Feel free to ask!

“This is really about re-imagining structural biology at scale,” Grigoryan said. “What could we do if it was just as easy to get the structure of a protein you’re working with as sequencing a piece of DNA?” #cryoEM #drugdiscovery #biotech

“As a result, the company will better understand the precise manner in which therapeutics interact with their intended targets, accelerating drug discovery processes and providing a competitive advantage in the de novo therapeutic protein field.” #CryoEM #drugdiscovery #biologics