@CollinsRNPLab profile picture
Collins Lab @CollinsRNPLab
Joined February 2024
  • Tweets 26
  • Following 145
  • Followers 235
10 Photos and videos
Collins Lab retweeted
SheqLab@ShechnerLab
29 Oct 2024
Beyond thrilled to present our O-MAP—now *published* @naturemethods (!!!) O-MAP is a powerful new method for biochemically "dissecting" the subcellular microenvironment around an RNA of interest, using off-the-shelf parts and standard manipulations. 🧵 nature.com/articles/s41592-0…

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Collins Lab retweeted
Akanksha Thawani, PhD
@AkankshaThawani
13 Nov 2024
Vertebrate retrotransposons are the future of gene therapy. But how do they insert their genes? 🔥🔥 Thrilled to present part 2 of my postdoctoral work with @NogalesLab @CollinsRNPLab @berkeleyMCB where we investigate this using #CryoEM and transgene insertion in 🧪 and cells!
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Hot off the presses!! How does R2 achieve its *exquisite* site specificity? Check out our new study led by former undergrad turned technician @rosajylee, now a PhD student @StanfordMed. cell.com/cell-reports/fullte…

Conserved and divergent DNA recognition specificities and functions of R2 retrotransposon N-termi...

Lee et al. elucidate general principles of target-site recognition by the N-terminal DNA-binding domains of site-specific R2 retrotransposon proteins across species and clades. They identify a...

cell.com
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Classical biochemistry will never die! We are ecstatic to share this work published in @NAR_open by graduate student Anthony @arodz_var that expands our understanding of RNA requirements for binding and TPRT by the R2 retroelement. A thread : academic.oup.com/nar/advance…

Distinct and overlapping RNA determinants for binding and target-primed reverse transcription by...

Abstract. Eukaryotic retrotransposons encode a reverse transcriptase that binds RNA to template DNA synthesis. The ancestral non-long terminal repeat (non-

academic.oup.com
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This work, combined with pre-existing knowledge of R2 biology, furthers our understanding of retroelement protein-RNA specificity. Additionally, it informs the design of retroelements as alternative tools for gene delivery.
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Of course we have to highlight the human behind the science! Anthony is a fifth year grad student in @berkeleyMCB. He is said to delight in the narcissistic love that retroelement proteins have for their own RNAs. We love you Anthony!!!
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News and views on our R2 PRINT story!
Nature Biotechnology
@NatureBiotech
18 Mar 2024
Replying to @NatureBiotech
A retrotransposon for site-specific gene transfer #NBTNV go.nature.com/4co04Ty
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Check out this exciting Berkeley News article by @bobthesciguy about our work! news.berkeley.edu/2024/02/20…

Junk DNA in birds may hold key to safe, efficient gene therapy - Berkeley News

New technique for inserting genes into a "safe harbor" in the genome would complement CRISPR gene editing.

news.berkeley.edu
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Collins Lab retweeted
Nature Biotechnology
@NatureBiotech
20 Feb 2024
Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci - @CollinsRNPLab go.nature.com/3utPERu
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Collins Lab retweeted
Kelly Nguyen@KellyTHD_Nguyen
20 Feb 2024
Groundbreaking study by the @CollinsRNPLab demonstrating the use of R2 proteins from multiple species for genome editing!
Connor Horton@connorh_sci
20 Feb 2024
Today starts a new era of genome engineering! We use RNA-only delivery to safely and efficiently deliver genes of interest to the human genome! @CollinsRNPLab @berkeleyMCB A thread 🧵: nature.com/articles/s41587-0…
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Great thread from our bioinformatics expert, Connor!
Connor Horton@connorh_sci
20 Feb 2024
Today starts a new era of genome engineering! We use RNA-only delivery to safely and efficiently deliver genes of interest to the human genome! @CollinsRNPLab @berkeleyMCB A thread 🧵: nature.com/articles/s41587-0…
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Bird bird bird, bird is the word! Is the secret to safe gene addition a retroelement in birds? We repurposed the avian R2 element to insert transgenes into human cells (and more!) using RNA-only delivery 🪶 a thread 🧵 nature.com/articles/s41587-0…

Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci

Nature Biotechnology - Transgenes are inserted into human cells by 2-RNA delivery of a retroelement protein and template.

nature.com
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PRINT is a complementary approach to existing methods for genome editing and we hope it provides new avenues for personalized medicine.
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This work took many years and many people to bring to fruition. We've come a long way from 1% GFP positive cells in October 2021!
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