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Dougan Lab @DouganLab

Regulated proteolysis | N-degron | AAA machines | #Proteostasis bsky.app/profile/douganlab.b… orcid.org/0000-0001-9313… views my own

Joined April 2015
  • Tweets 4,464
  • Following 867
  • Followers 922
61 Photos and videos
Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex | Nature Communications nature.com/articles/s41467-0…

Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity...

Nature Communications - Transcriptional regulation is a central mechanism underlying bacterial adaptation to stress. Here, the authors used a genetic fitness screen to show that the Y-complex in...

nature.com
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Molecular Cell @MolecularCell
May 26
Online Now: Design principles of a membrane-spanning ubiquitin ligase dlvr.it/TSkgS6
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A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS pnas.org/doi/10.1073/pnas.26…

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short-...

The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

pnas.org
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Molecular mechanisms of mitochondrial AAA proteases - Journal of Biological Chemistry jbc.org/article/S0021-9258%2…

Molecular mechanisms of mitochondrial AAA proteases

Mitochondrial AAA proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association...

jbc.org
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Chemical activation of mitophagy via the N-degron pathway alleviates mitochondrial neuropathies biorxiv.org/content/10.64898…
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Dougan Lab retweeted
Guillaume Laconde @Glaconde34
Feb 9
Data-Driven Design of PROTAC Linkers to Improve PROTAC Cell Membrane Permeability | JACS Au pubs.acs.org/doi/10.1021/jac…

Data-Driven Design of PROTAC Linkers to Improve PROTAC Cell Membrane Permeability

Proteolysis-targeting chimeras (PROTACs) are promising next-generation therapeutics for the degradation of disease-associated proteins. However, optimizing the physicochemical properties of PROTACs,...

pubs.acs.org
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Molecular mechanisms of mitochondrial AAA proteases - Journal of Biological Chemistry jbc.org/article/S0021-9258%2…

Molecular mechanisms of mitochondrial AAA proteases

Mitochondrial AAA proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association...

jbc.org
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Dougan Lab retweeted
Hema Chandra Kotamarthi @HemaKotamarthi
Feb 4
Our recent work on mechanism of disassembly and reassembly of oligomeric proteins is now published in ACS Biochemistry. doi.org/10.1021/acs.biochem.…

Concerted Deoligomerization and Nonfunctional Reassembly of the Hexameric Proteasomal ATPase Mpa...

Mycobacterium tuberculosis Mpa is an AAA ATPase that unfolds and translocates substrate proteins during proteasomal degradation. Mpa spontaneously assembles into a homohexamer driven by interdomain...

pubs.acs.org
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CLIP-LUX: A Luminescent Split-Ubiquitin Assay Using ClpS-Mediated N-Degron Recognition for Detection of Protein-Protein Interactions in Mammalian Cells. sciencedirect.com/science/ar…
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Elongation Factor Tu Acts as a Chaperone to Activate an Antibacterial RNase Toxin - goo.gl/scholar/WFsfuJ #ScholarAlerts
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Molecular Cell @MolecularCell
Jan 28
Online Now: Mitochondria as sources and targets of cellular signaling dlvr.it/TQcy31
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ShorterLab @ShorterLab
Jan 27
Metabolically regulated proteasome supramolecular organization in situ: Cell cell.com/cell/fulltext/S0092…

Metabolically regulated proteasome supramolecular organization in situ

Structural steps along the assembly of proteasome storage granules—membraneless organelles that form in response to metabolic shifts in yeast—are visualized inside cells by cryo-electron tomography....

cell.com
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Dougan Lab retweeted
Molecular Cell @MolecularCell
Jan 27
Online Now: Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors dlvr.it/TQbRjK
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The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis sciencedirect.com/science/ar…
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ShorterLab @ShorterLab
28 Nov 2025
In @eLife: Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (PROTAC) doi.org/10.7554/eLife.101496

Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (p97-PROTAC)

Synthetic adaptors combining camelid nanobodies with the UBX domain of the p97 adaptor FAF1 enable targeted substrate delivery to p97, establishing an E3 ubiquitin ligase-independent mechanism for...

elifesciences.org
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Dougan Lab retweeted
ShorterLab @ShorterLab
6 Nov 2025
Structural landscape of the degrading 26S proteasome reveals conformation-specific binding of TXNL1: nature.com/articles/s41594-0…

Structural landscape of the degrading 26S proteasome reveals conformation-specific binding of TXNL1

Nature Structural & Molecular Biology - The authors use time-resolved cryo-electron microscopy to reveal the interactions of the redox-active cofactor TXNL1 with the human 26S proteasome and...

nature.com
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MITOtalks @MitOtalks
4 Nov 2025
How's your juggling act? Mitochondria also have one, and the MITOtalk this week will be all about it. See you there?
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Dougan Lab @DouganLab
30 Oct 2025
Mechanisms of assembly and function of the Hsp70-Hsp40 chaperone machinery sciencedirect.com/science/ar…
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Dougan Lab retweeted
Molecular Cell @MolecularCell
20 Oct 2025
Online Now: Delayed protein translocation protects mitochondria against toxic CAT-tailed proteins dlvr.it/TNnWzh
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Cameron Thrash @jcamthrash
22 Oct 2025
Proteolytically activated antibacterial toxins inhibit the growth of diverse gram-positive bacteria pnas.org/doi/abs/10.1073/pna…

Proteolytically activated antibacterial toxins inhibit the growth of diverse gram-positive bacteria...

Many species of bacteria produce small-molecule antibiotics that enter and kill a wide range of competitor microbes. However, diffusible antibacter...

pnas.org
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