Beyond Static Screens: A High-Throughput Pooled Imaging CRISPR Platform for Dynamic Phenotype Discovery biorxiv.org/content/10.1101/… #biorxiv_cellbio

Better tools = better science = better medicines. 🧬✨ 💡THE IDEA: create a huge mixed batch of cells where each cell has a different gene switched off. Then scan through them under a microscope, looking for anything unusual and when you find it, mark it with a laser so you can pick it out later. 👎THE PROBLEM: the laser light was too intense meaning the tag was switching on and then immediately burned out. 👍THE FIX: turn down the laser power, reduce how long it lingers on each spot, and slightly blur the focus. Simple tweaks but the result is cells that glow much more brightly and reliably helping scientists accurately track cells of interest. Interested in learning more? Have a look at the Research Protocol from @sravmukherjee , Giulia Zanetti, Bram van den Broek, @jalink_kees from @NKI_nl and @UvA_Amsterdam 🔗febs.onlinelibrary.wiley.com…

Our latest preprint from the lab is now OUT!!! 🧫🔬 Spearheaded by our PhD student Giulia Zanetti - we made cytosolic-enhanced dedicated FLIM sensors for intracellular cAMP detection! Check it out and a small thread below 👇

Back in June this year, I had the opportunity of giving a talk at the 17th @ipscconf organized by the MDC in Berlin!!! I also won the best talk award which was the cherry on top!!! 🤓 Thanks to the organizers for a great conference!!! Look forward to next year!! 🥳