newest collaboration with Zhao Lab, led by Xiaomeng Shen, uncovers how KRN5b regulates maize kernel row number through phosphoinositol signaling! I feel so satisfied that our whole-mount immunolocalization protocol worked like a champ with the “oldie-but-goodie ” PIN1 antibody.
ALT KRN5b regulates the maize kernel development through its influence on hormones. (h–n) fluorescent PIN1immunohistochemistry signals in immature ears of control (h–j) or KRN5b KO (k–n). (h) PIN1 immunolocalisation in the cross section of control ear. (i and j) PIN1 immunolocalisation (red in i and j) merged with DAPI (blue in j) in the vertical section of control ear.
ALT KRN5b regulates the maize kernel development through its influence on hormones. (k) PIN1 immunolocalisation in the cross section of KO ear. (l and m) The local features of (f) shows the normally developed FM and the arrested kernel primordium.