For anyone interested in using portable DNA sequencing with Oxford Nanopore MinION as a teaching and capacity-building tool in conservation and biodiversity research, here is a great article by Watsa et al. (2020) with training advice, case studies, step-by-step protocols, and lots more!
It's also worth checking out the supplementary information for equipment lists, a generalized course syllabus, protocols and pipelines, and a post-course questionnaire.
You can read the article here:
Watsa et al. (2020). Portable sequencing as a teaching tool in conservation and biodiversity research. PLoS biology, 18(4), e3000667.
journals.plos.org/plosbiologβ¦
You can also read all the protocols on
Protocols.io:
protocols.io/view/field-genoβ¦
And, since field sequencing methods advance quickly, it's also worth checking out a more recent article on field sequencing by Pomerantz et al. (2022):
Pomerantz et al. (2022). Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment. Nature Protocols, 17(6), 1415-1443.
researchgate.net/publicationβ¦
And an even more recent article by Srivathsan et al. (2024) featuring real-time DNA barcoding via ONTbarcoder 2.0:
Srivathsan et al. (2024). ONTbarcoder 2.0: rapid species discovery and identification with realβtime barcoding facilitated by Oxford Nanopore R10. 4. Cladistics, 40(2), 192-203.
onlinelibrary.wiley.com/doi/β¦
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journals.plos.org/plosbiologβ¦