Postdoctoral scholar in @veeslerlab @UW. Former @UCSFBiophysics @SFSU & @ucsc & @exploratorium explainer. Tiny pictures.
Joined March 2017
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34 Photos and videos
20 Oct 2024
As we celebrate the PDB, let's also remember the prolific but underrecognized structural biologists who contributed so much to our field, and to us personally as trainees. Scientists like @UCSF @UCSFBiophysics legend Janet Finer-Moore, with 125 depositions from 1987 to 2024! ππ₯³
20 Oct 2024
Happy Birthday PDB, announced #OTD in 1971 In Nature New Biology.
Today we celebrate the 53rd anniversary of the Protein Data Bank and the PDB depositor community, and congratulate the 2024 Nobel Prize in Chemistry Awardees
rcsb.org/news/feature/670ffeβ¦
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20 Sep 2024
Hey cryo-EM tweeps - I wonder if anyone has seen anything weird with significant 3xFLAG peptide in your samples? (Like 100 uh/mL after eluting)
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20 Sep 2024
The uh is of course the unit of confusion used to quantify bad grids (ug)
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Daniel Asarnow retweeted
20 Aug 2024
πExciting news!π Iβm thrilled to share our latest preprint, which marks a special milestone. This manuscript, authored by my brilliant PhD student @ciara_pugh is her first scientific paper with my group and presents the first high-resolution cryo-EM structure from my group!
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Daniel Asarnow retweeted
9 Jul 2024
How many times did coronaviruses evolve the ability to use ACE2 as receptor for infection? Find out by reading this @biorxivpreprint in collab with Huan Yan (Wuhan Univ)
Led by Ma CB, Liu C @YoungjunPark11 Tang J & Chen J
@HHMINEWS
@UWBiochemistry
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biorxiv.org/content/10.1101/β¦
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9 Apr 2024
Two Mondays, two Salems, two eclipses - no witchcraft
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Daniel Asarnow retweeted
1 Apr 2024
Sharing our work on developing counter-measures against porcine-deltacoronavirus (PDCoV) which is also infecting humans!
Led by @RexhepajMegi @YoungjunPark11 & Lisa Perruzza w Fabio Benigni
@HHMINEWS
@UWBiochemistry
biorxiv.org/content/10.1101/β¦
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14 Mar 2024
Nice idea - I've done something quite similar in 3D, using particle orientation. In general I find it doesn't improve the resolution or replace 3D classification, but large proportions of particles (~3/4 in some cases) can be excluded with no loss.
22 Jan 2024
Duplicated Selection To Exclude Rubbish particles (DuSTER) is a method for facilitating the structural analysis of small biomolecules. DuSTER uses the reproducibility of the particle centering during 2D classification to exclude the low S/N. biorxiv.org/content/10.1101/β¦
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15 Mar 2024
Here's my poster about it (thought the code was already buried in my github somewhere, but not so, I'll have to post it later)
github.com/asarnow/posters/bβ¦
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11 Mar 2024
If these phenotypes worry you... nucleofection maybe?
9 Mar 2024
π " moment of the entry of Lipofectamines into the cell via endosome-rupture"
So many wonderful cell biology ideas behind how John Heuser captured these rare, transient, asynchronous moments. Read them here:
early #EM_Monday @HaniehFalahati
biorxiv.org/content/10.1101/β¦
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6 Mar 2024
"Deliberately and for private reasons" π
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Daniel Asarnow retweeted
28 Feb 2024
CTFFIND5 provides improved insight into quality, tilt and thickness of TEM samples biorxiv.org/cgi/content/shor⦠#biorxiv_biophys
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27 Feb 2024
Paul Doherty taught me the Nakamura lock 20 years ago @exploratorium & I'm sure I'm not the only one who remembers him for it (any many other things) π
26 Feb 2024
How to make an effective paper plane
[πΉ Fuzail Ansari / mr.fuzail_art]
twitter.com/i/status/1762192β¦
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27 Feb 2024
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9 Feb 2024
We used to talk about this idea in @yifan_ucsf lab, but my lab mate chose to start with hydrophobins and never got past initial playing-around. So cool to see folks actually tried it and it works!
1/6: #CryoEM Exhausted with fighting the air-water interface (AWI) problem during sample grid plunge freezing?
We may have an easy solution for you!
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: biorxiv.org/content/10.1101/β¦
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24 Jan 2024
This is like saying debunking the Laffer curve is pointless, because it merely reflects a preexisting political conclusion
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24 Jan 2024
It's important to say that incorrect, politically motivated reasoning is motivated AND that it's wrong - because otherwise it's going to be used again and again in service of the same politics
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8 Jan 2024
This is cool but feels like the real lesson is that @RolandDunbrack rocks
5 Jan 2024
One bead per residue can describe all-atom protein structures dlvr.it/T10wLK
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8 Jan 2024
Unless I'm reading it wrong, the state of the art neural network is performing slightly better than a program the Dunbrack lab first published over 25 years ago
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22 Dec 2023
When foundational discovery turns into "common knowledge" so quickly, the ink is hardly dry before folks argue whether to even cite a source, we risk forgetting what made a new idea challenging - why we believed in the old paradigm and what kind of thinking can change the game
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22 Dec 2023
Tonight I'm remembering a lecture (Biochem 100C, Spring '08) where we discussed the rise and fall of what he called the "Orthodox model" over this then-recent press release
newsarchive.berkeley.edu/newβ¦
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22 Dec 2023
TL;DR Bob Ludwig was a great teacher, emblematic of how greatly @UCSCscience values pedagogy, and also colocalization of lactate dehydrogenase and the malate antiporter is still a big deal, if it's 15, or 30, or (probably) a hundred million years later
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