It’s my pleasure to share that our latest manuscript is out now in Nature Biotechnology. This has been an incredibly exciting project! We describe BreakTag, an NGS-based tool for the rapid characterization of CRISPR nucleases and off-target activity. nature.com/articles/s41587-0…

Today in @Nature we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, scalable, and mutation-agnostic genome editing. 🧬 🧵(1/13) nature.com/articles/s41586-0… @BKleinstiver @CGM_MGH @MassGeneralNews @harvardmed

New Article! Multilevel characterization of genome editor nuclease activity with BreakTag bit.ly/4o0SFOD

.@SecKennedy - thank you so much for your broad support for gene editing and gene therapies as well as recognizing the importance of Baby KJ’s story and the potential that CRISPR base editing therapies hold for patients with severe genetic diseases. I’m thrilled you have publicly recognized the Herculean efforts that made KJs therapy possible, from the physicians @CHOP like @kiranmusunuru and Rebecca Ahrens-Nicklas to the technological innovation in @davidrliu and @BKleinstiver labs, and especially the coordination and cooperation of the regulators @US_FDA that made this possible. The gene editing medicine KJ received, a CRISPR adenine base editor was invented and developed in @davidrliu lab @Harvard with government grants from the NIH. This life-saving medicine, and so many future technologies, could not and will not be possible without government grants such as the one that supported this work, but are now shut off by this administration. So PLEASE ask this administration to stop attacking our academic institutions and restore government funding to @Harvard (where again, KJ’s medicine was created!!) We cannot have stories like KJ’s without full and unwavering support from the US government. Please help, please.

Really nice characterization of Cas9 DSBs in human gDNA, with thorough off-target profiling and modeling of DSB outcomes for different guides. From @genomewizard @VassilisRozanas

The genome-wide landscape of Cas-induced double-strand breaks and end structures is profiled at nucleotide resolution go.nature.com/44DO2Cc rdcu.be/ekKtv

In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-Cas9 enzymes, offering safer & more genome editing tools. 🧬🖥️ @RachelSilvers11 @CGM_MGH @MGH_RI @MassGeneralNews @harvardmed nature.com/articles/s41586-0…

A scientist who successfully treated her own breast cancer by injecting the tumour with lab-grown viruses has sparked discussion about the ethics of self-experimentation go.nature.com/3NXqzEq

Our Roukos lab discovered that cells use type II topoisomerases to regulate the 3D folding of DNA. This is important for controlling gene expression & may also be important for preventing DNA from becoming overtwisted & breaking. Findings published in Molecular Cell @cellpress.

Publication alert! 📰 Great job to @RoukosVassilis and collaborators on the new publication in @MolecularCell! Read more about it here: cell.com/molecular-cell/full…

The @RoukosVassilis lab led a new and detailed study of Topoisomerase II contribution to #3Dgenome folding in human cells and in different cell cycle phases -- my group and the @MundlosLab contributed analyses/data. The paper is now published in @MolecularCell ...1/n

Happy to share our new work in collaboration with the @MundlosLab @StefanMundlos & @AkisPapantonis labs, published today in @MolecularCell Many congratulations to all co-authors and many thanks to the funding agencies for the important support @imbmainz @sfb1361 @upatras