Sable is excited to announce our new Promethion Core® XT Behavioral Measurement System, expanding the world-leading accuracy & flexibility of the Promethion Core® from 24 up to 96 rodent cages! buff.ly/E2pTxda #Metabolism #Physiology #Respirometry #IndirectCalorimetry

ナノポアPromethIONフローセルのランのみもできます。手技等が気になる方はラボまでお越しください(｀・∀・´)

Chinese study: A pilot study on the application of Oxford Nanopore PromethION sequencing with adaptive sampling for DMPK repeat expansion sizing in myotonic dystrophy type 1 sciencedirect.com/science/ar…

Tuve la suerte de participar la semana pasada en London Calling 2026, de @nanopore, y una de las novedades más relevantes fue la presentación de PromethION Plus Flow Cell. El mensaje es simple, pero muy importante para quienes pensamos en escalabilidad: más output, mayor yield y menos fricción operativa, sin agregar pasos de wash/reload. En secuenciación de alto rendimiento, la diferencia no está solo en generar más datos. Está en hacerlo de forma más predecible, con menos intervención manual y con workflows más fáciles de integrar en entornos donde la reproducibilidad importa. Para genómica humana, cáncer, enfermedades raras, estudios poblacionales y aplicaciones clínicas translacionales, este tipo de mejora puede tener un impacto directo: más capacidad por corrida, mejor aprovechamiento del instrumento y una vía más clara hacia estudios de genomas y metilomas completos a escala. #OxfordNanopore #PromethION #LongReadSequencing #Genomics #Multiomics #NanoporeConf #LC26

How do #cardiomyocyte signals contribute to #MyocardialFibrosis in #heart disease? With help from Sable Promethion Core #Metabolic Cages, scientists studied the role of casein kinase α (#CK2α) in disease progression. buff.ly/swW2t4i #Mitochondria #CVD #Metabolism

共同研究をさせていただいている鍋島先生のご発表です ----------------------------------------------- Nanopore PromethIONを用いた野生動物ゲノム解析：新規ゲノム決定から保全・感染症研究への展開 nanoporetech.com/about/event… 鍋島先生は、日本をリードする野生動物ゲノミクスおよび保全ゲノミクスを専門家としてパワフルに研究を進めていらっしゃいます♪ ご興味のある方はぜひぜひ♪ @4sotY6ZmydH4bUK

I want to loan / buy a PromethION 2 Solo, so I can do 30x WGS at home. I want to record myself running the full sequencing protocol end-to-end, and post the video. If you are open to this, or know of somehow who may be, please DM me.

The installation of @nanopore PromethION 24 was celebrated with stakeholders over Pizza and cake. Long read enabled questions of structural variants, methylation, repeats & novel bacteria discovery, in addition to the canonical ones....@IGIBSocial

A new powerhouse of Genomics has now joined the #INGENHOPE lab at @IGIBSocial.... looking at generating more than 4 Terra bytes of long read data per run, welcome @nanopore PromethION 24 for solving biological problems

I’m so sad this device was cancelled - I almost used the PromethION 2 SOLO instead of the MinION for my run so I could get 30x coverage across the entire genome. Didn’t manage to source one unfortunately. I just hope the MinION doesn’t go the same way…

New capability unlocked. Our team trained on the newly acquired PromethION 24, advancing high-throughput sequencing and strengthening genomic surveillance for global health. #Innovation #IGHResearch

ナノポアのライブラリー持ち込みでの、PromethIONのランのみも実施していますので、MinIONでは間に合わない時にどうぞ✨

Adaptive sampling for a target panel gets you comfortably to 30x coverage for the panel. Will do another MinION cell or maybe PromethION cell for the next run next week

Adaptive sampling with a targeted to start - gets you to 30x coverage for the panel. Will either use another flow cell for the MinION or use the PromethION for the next run to get full 30x coverage

#Irisin, an #exercise-induced #myokine, counters #obesity via effects on #adipose tissue — but how? With help from Promethion #Metabolic Cages, a Nature #Metabolism study has shown that irisin modulates adipose #IL33 levels to increase #thermogenesis. buff.ly/S5YguKA

Note the gorgeous, gorgeous (did I say gorgeous?) Promethion metabolic & behavioral phenotyping system. A surefire sign of a leading scientific research group!

If you have unlimited resources, supplement long-read data with very high depth short-read sequencing, due to often-superior error rates. Sequence to 1000x depth for better resolution of within-sample mosaicism. There are likely also single-nucleotide variants differing between twins. Apply Sanger sequencing to confirm each short-read WGS-identified variant, as done in real-world applications, given those alleles have a high allele fraction. Using both SNV-based and structural variation-based results gives extra confidence if the two approaches agree. Quantify the strength of evidence by modifying the likelihood ratio from Krawczak et al. (2018), "Distinguishing genetically between the germlines of male monozygotic twins," which operates on read counts and can account for multiple tissue types (doi.org/10.1371/journal.pgen…). At high read depth, bias from sample contamination could invalidate the read count estimator, so cap the maximum likelihood contribution from a single variant or something. Validate the pipeline using genomic data in All of Us and the UK Biobank, both of which include cohorts with short- and long-read sequencing, to test the method with twins and children of known relationships. This calibrates confidence estimates and informs how sure you can be in the paternity result. If follow-up analyses warrant it, sequence the mother to resolve ambiguities from maternal transmission or to confirm phasing, optionally combined with Strand-seq on the child's genome to improve assembly contiguity for reference-free structural variation analysis. For low allele fraction mosaic variants, use targeted unique molecular identifier amplicon sequencing to validate SNVs, which is more robust to PCR and sequencing errors. The primary variants of interest are discordant sites from triplicate sperm samples between the twins compared against the child's genome, followed up with PCR and Sanger sequencing for SNVs and small indels, and optionally droplet digital PCR for copy number variation differences. If PacBio HiFi plus ultra-long Oxford Nanopore assemblies suggest large higher-order repeat variation, use optical genome mapping or optionally pulse-field gel electrophoresis followed by Southern blot as confirmation. Cost estimate of extreme version: 1000x WGS of the human genome is 3.2 Gb × 1000 = 3,200 Gb per sample; 100x is 320 Gb. PacBio Onso short-read sequencing runs about $772k total across blood, triplicate sperm from each twin, and saliva, using 300-cycle kits at roughly 120 Gb per flow cell. PacBio HiFi long-read WGS at 100x depth across two sperm and one blood sample runs about $19k. Oxford Nanopore ultra-long reads at roughly 50 Gb per PromethION flow cell for three samples runs about $41k. Optical genome mapping on three blood samples adds about $6k. PCR and Sanger sequencing for 30 loci across three samples, forward and reverse, comes to about $1k. Total: approximately $840,000. DNA extraction is completed by asking very nicely for someone with relevant wet lab experience to do it before sending out samples, so that part is free. Optional contingent components like Strand-seq, sequencing the mother, and ddPCR are omitted unless ambiguity arises.

【論文紹介】　ナノポアだけでヒトT2T “Here, we present a streamlined Nanopore-only workflow for diploid human T2T assembly using three ultra-long and one Pore-C PromethION flow cell per individual.” たったこれだけのフローセルで！？😆

From plasmids to AMR genes — resolve it all in one workflow. In this webinar, discover the updated NO-MISS microbial isolate sequencing workflow, now supporting PromethION for high-throughput projects. nanoporetech.com/about/event…