Wunderbar! Our Crossbeam just showed up this week, so I'm looking forward to getting serialFIB running on it.

We listened to the community and here is the result: Got a CrossBeam that you would like to use for automated milling? The driver for lamella workflows in SerialFIB (github.com/sklumpe/SerialFIB…), developed in collaboration with @zeiss_micro , has now been tested on 2 Crossbeams

Multiple software (xT, MAPS, AutoTEM) has many functions beyond those needed for cryo-FIB, thus they can be put in background with a single minimalist frontend like SerialFIB. Manufacturers' innovations will be rewarded as easier & cheaper cryo-FIB will lead to more investments

Not many #PFIB #Arctis systems out there but maybe useful to some: #SerialFIB runs on Arctis now #TeamTomo!AutoLoader integration will change in the future and is a little hacked with help from my @thermosciEMSpec friends, thus not on GitHub, PM if needed. github.com/sklumpe/SerialFIB…

Already described in plastic-embedded samples before but really helps in native samples without staining in cryo-(P)FIB-SEM. SerialFIB scripts for 90 degree imaging available on github dev branch (github.com/sklumpe/SerialFIB…). YouTube tutorial to follow after vacation 😅

Interested in pFIB-SEM? Check out this paper by Maud Dumoux, @mcgrange et al. : biorxiv.org/content/10.1101/… . Happy I could help out with some SerialFIB scripting! Want high contrast and quality images in cryo-FIB-SEM tomography? Imaging at 90 degrees helps! #vEM #FIBSEM #PFIB.

On a side note, obviously the moment we have this up and running we will be developing a driver for SerialFIB (GitHub.com/sklumpe/SerialFIB) that communicates with it, because #OpenSoftwareAcceleratesScience . Also some other machine drivers maybe in the making 😉#TeamTomo #PFIB #FIB

SerialFIB enables the streamlining of advanced cryo-FIB protocols such as multi-modal imaging, CLEM-guided lamella preparation and in situ lamella lift-out procedures. elifesciences.org/articles/7…

At the moment, there is no dedicated software able to integrate all methodologies included in cryo-FIB. SerialFIB is an adaptable #OpenSource software that aims to alleviate this problem and eliminate the need for proprietary software. elifesciences.org/articles/7…

I last week's #journalclub we looked at how cryo-FIB workflows are automated in SerialFIB: elifesciences.org/articles/7…

Want to use SerialFIB but reading tutorial not your thing? Here is the video version

In addition, check out the cryoFIB live session YouTube tutorial on the usage of SerialFIB: youtube.com/watch?v=QR7ngJ0a… . There will likely be more videos on the channel in the future whenever new major functionalities have been implemented.

=) Our lab will be in contact with you. @ashleighraz has SerialFIB installed on our Helios and auto-waffles are exactly what we want to do!

#SerialFIB🤖 #OpenSoftwareAcceleratesScience🙌

Finally scripting on the FIB again this morning and did my first attempt at automated Waffle using SerialFIB (Perpendicular cut, nodge lamella) during the #MandM2021 sessions. Needs some optimization and real world trial in cryo, but I like my wanna-be copper lamellae 😁

Yes! AutoTEM waffle lamellae thinning is now routine at @NCITUinfo. We are looking into SerialFIB. It seems like it may be possible to automate all of waffle milling, from trench milling to notch milling to thinning, and all the rotations too. Do you agree?

SerialFIB (github.com/sklumpe/SerialFIB) manuscript talked about the ability to do that @svenklumpe. I don't have any projects for HPF so will have to let others comment.

Amazing work - SerialFIB!! 🤩

First there was SerialEM, now there is #SerialFIB!! Fantastic work by @svenklumpe et al. and a great tweetorial too! Check out that Chlamy cryo-FIB/SEM volume imaging and that stunning Drosophila🪰 lift-out! This is a very important #OpenSource solution for automated FIB milling.