Randomized PatchSeq until it sounded interesting :) 3-14-26d
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PatchSeq puts a FM-filled, sequenced patcher inside your @vcvrack - review (and a tip or two)!
cdm.link/patchseq-vcv-rack/
It, uh, makes other sounds, too...
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Jan 11
And so the mayhem begins. With @vcvrack PatchSeq (Voxglitch Jeremy Wentworth collab) -- more on this shortly.
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PatchSeq Coming Soon! A collab with Voxglitch
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24 Dec 2024
I can only stress how much fun this fruitful collaboration was and still is. Thank you @neuronJoy and @internautderek! Looking forward to hearing your comments #paincommunity #patchseq #ionchannel #pain #itch #microneurography #transcriptomics
23 Dec 2024
Happy Holidays! I’m really excited to share this preprint on using Patch-seq and other multi-modal methods to identify the molecular identity of silent nociceptors in the dorsal root gangion in pigs and humans! biorxiv.org/content/10.1101/… 1/
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12 Sep 2024
Looking forward to the meeting and also our symposium on PatchSeq in sensory neurons (central and peripheral) with Shreejoy Tripathy @neuronJoy @EckhardFriauf and Cathryn Cadwell @crcadwell. See you there!
The Website of the 16th Göttingen Meeting 2025 is open now: nwg-goettingen.de/2025/defau…
Take the opportunity to enrich the conference with your abstract and actively shape it!
The Deadline for Early Registration, Abstract Submission and Stipend Applications is:
10 October 2024.
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Fantastic talk by @LaurenJDonovan from @TawfikLab at the NeuroImmune session at @IASPpain world congress! Huge shoutout to superstar Baccei Lab alum @ChelsieLBrewer for doing some sweet PatchSeq studies in the DRG (crazy hard stuff!)
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16 Jul 2024
what a fantastic seminar we had by @LampertLabRWTH, Dr. Angelika Lampert on her latest work on sodium channel mutations and PatchSeq approaches! Thank you for the amazing science!
15 Jul 2024
Happens today at 16:00 Eastern. Be there.
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19 May 2024
(1/11) Andrés Crane’s thesis work in the lab with Suresh Jetti using PatchSeq to define the targets and rules for single neuron RNA editing in Drosophila motoneuron subtypes is now available as a preprint:
biorxiv.org/content/10.1101/…
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26 Nov 2023
4. We aspirate the intracellular content into a PCR tube.
5. We proceed with an RNA-seq protocol for each PCR tube (cell).
6. We can perform immunohistochemistry on the remaining tissue section.
More details
nature.com/articles/nbt.3445
nature.com/articles/nbt.3443
#patchseq #neurons
2/2🧵
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8 Nov 2023
Thank you @bdxneuroschool for counting on me as an instructor for this #CAJAL course. I hope my students think now that PatchSeq is not that scary 🧬🔬 It was an amazing experience!
📸Group photo with all the participants of the #Cajal course on Advanced Techniques for #Synapse #Biology
@AnaLuisaCarva
@letelliermathi1
@Cajal_Training
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Our patchseq data has been used in a few papers. Nice to see it used here to show differences in Na(v) current in beta cells depending on HNF1A expression.
We’re actively working to make this and other data more broadly usable and available. Stay tuned.
nature.com/articles/s41467-0…
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22 Aug 2023
12/12 Challenging Isoform-Patchseq experiments are possible due to generous support from my postdoc advisor @JTroyLittleton and previous in vivo patch training in Zebrafish neurons. Thanks also to my PhD advisor @yaksi_emre for mentoring challenging in vivo patch experiments!
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22 Aug 2023
5/12 To characterize molecular differences that underlie synapse diversity, I established Isoform-Patchseq in collaboration with core facilities at @MIT. Special thanks to @Scientifica_Int manipulator which made my life easy to collect these challenging patch-sep samples!
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22 Aug 2023
@suresh__jetti and colleagues paper from the lab on PatchSeq approaches to characterize differences in Drosophila tonic-like Ib and phasic-like Is motoneurons is now published in Neuron – check it out from the link below.
doi.org/10.1016/j.neuron.202…
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21 Jan 2023
Fantastic work by Suresh et al. from the Littleton lab @JTroyLittleton ! The painstaking, rigorous PatchSeq RNA profiling of our favorite neurons will be foundational for some of our future studies. And I'm sure other labs will also greatly benefit. Thank you!
20 Jan 2023
Suresh and colleagues’ work in our lab on characterizing Drosophila tonic (Ib) and phasic (Is) larval motoneuron (MN) properties and their associated transcriptomes is now out in BioRx, along with supplementary material valuable for the field.
biorxiv.org/content/10.1101/…
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20 Jan 2023
Challenging Drosophila larval motoneuron Isoform-Patchseq experiments are possible due to my previous training in zebrafish in vivo patch-clamping. Thanks to my Ph.D. advisor @yaksi_emre for his mentoring and passion for teaching electrophysiology
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20 Jan 2023
Our Drosophila larval motoneuron Isoform-PatchSeq print is out! Thanks to @JTroyLittleton and other for their collaboration.
Molecular Logic of Synaptic Diversity Between Drosophila Tonic and Phasic Motoneurons biorxiv.org/content/10.1101/…
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20 Jan 2023
We’ve only begun to scratch the surface of the biology that can be gained from Suresh’s Isoform-PatchSeq data sets, but hopefully others in the field will find it useful as they explore their favorite molecular pathways at Drosophila larval NMJs.
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