Post-doc @UMich in the @KoschmannLab. Research: intro-operative/rapid sequencing, high-accuracy cf-tDNA sensing. @nanopore hacker. He/him 🏳️🌈
Joined June 2011
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Pinned Tweet
18 Jul 2022
SUPER excited for this project to finally be out in
@CommsBio! Important updates since the pre-print include replicates, flowcell re-use, live basecalling/analysis, and ddPCR validation. @nanopore will end up in-OR (not if but when). A few notes below...
18 Jul 2022
Somatic variants for the intraoperative evaluation of tumor mutations can be detected in less than 30 minutes, using rapid DNA extraction, in silico optimized LAMP amplification, and a LAMP product analysis tool: LAMPrey. @jackwadden nature.com/articles/s42003-0…
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17 Nov 2023
Such an amazing experience to present to this group at #SNO2023! Come say hi at my poster later (BIOM-58) to talk about how we are rapidly and accurately sequencing a variety of sample types (tumor, csf, blood, biopsy wash water) using @nanopore
17 Nov 2023
Excellent Presentation by @jackwadden from @KoschmannLab at SNO2023 (@NeuroOnc) on Nanopore sequencing plasma for H3K27M!
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Jack Wadden retweeted
22 May 2023
Fanstastic line up of insightful talks at the first ever @UMRogelCancer #liquidbiopsy symposium by @jackwadden @andi_cani @TewariLab with special guest speaker @max_diehn
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19 May 2023
Even after one day, I'm *SO* happy I came to #NanoporeConf. Amazing networking. If you're here, come nerd out with me about hyper-accurate concatemeric consensus seq, ultra-rapid (<30min) seq, weird isothermal amplification techniques, and liquid biopsy using @nanopore!
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19 May 2023
I'll be hanging around my poster (#59) from 11:30-12:40. We're showcasing our approach for MRD and cancer monitoring. Concatemer consensus sequencing is so accurate we can track VAFs down to ~0.05%
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19 May 2023
In fact, CCS is so close to perfect that we are able to profile the error rate of *individual pores*. It turns out not all pores are created equally and politely excusing the slackers further improves accuracy
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19 May 2023
Day two at #NanoporeConf! Kicking things off listening to the Data for Breakfast talks. Kudos to @nanopore for committing to open sourcing their code! About halfway through adding support for duplex basecalling of lamplicons to dorado for even more accurate cf-tDNA detection
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18 May 2023
Excited to listen to @billytcl, @Carolinmsa, and @adbeggs talk about utility of @nanopore for liquid biopsy #NanoporeConf #LC23
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18 May 2023
Here we go! So excited to be at my first London Calling. I’m here presenting my poster (#59) on our same-day, LAMP-based ctDNA RMD monitoring technique. Come say hi! I’m the one with the big scar on my head @nanopore #NanoporeConf
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Jack Wadden retweeted
4 May 2023
The incredible Drs. @CarlKoschmann &
@NazarianLabs presenting at the @PNOCFoundation @PNOC_kids @act_dmg in SF! So proud!
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Jack Wadden retweeted
19 Apr 2023
At #AACR23, @jackwadden shared his research developing a new protocol to identify brain tumours in less than an hour, right in the operating room. He discussed his protocol's potential to impact surgical-decision making, in the future. Learn more👇
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17 Apr 2023
Super excited to be at #AACR23 presenting my poster on needle wash diagnostics. We’re sequencing cell-free waste water DNA from biopsy needles and finding exciting potential for clinical diagnostics *AND* research. Come say hi at section 38 poster 18 (#2115)
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17 Apr 2023
Featuring some older ultra-rapid LAMP @nanopore results. We were able to amplify DNA from raw, unprocessed wash water and call clinically relevant mutations within 1hr of sample hands on! We can go a lot faster these days 😜
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4 Apr 2023
Briefly switching gears from my liquid biopsy work back to ultra-rapid @nanopore sequencing. This is a three target panel with 1,000x coverage over all three targets ***within 30mins*** of tissue hands on. Continuously amazed by this tech and kit14 improvements!
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22 Mar 2023
Also was able to ask why they deprecated 260bp/s models in favor of 400bp/s or higher speeds. Answer seemed to boil down to: market for super high accuracy but low throughout was small. Unfortunately this is me rn.
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22 Mar 2023
Outy sequencing advancing nicely. Was always curious if this was possible with short fragments. He’s showing data from 176bp sequence! That would be a game changer.
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18 Jul 2022
SUPER excited for this project to finally be out in
@CommsBio! Important updates since the pre-print include replicates, flowcell re-use, live basecalling/analysis, and ddPCR validation. @nanopore will end up in-OR (not if but when). A few notes below...
18 Jul 2022
Somatic variants for the intraoperative evaluation of tumor mutations can be detected in less than 30 minutes, using rapid DNA extraction, in silico optimized LAMP amplification, and a LAMP product analysis tool: LAMPrey. @jackwadden nature.com/articles/s42003-0…
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18 Jul 2022
13) SOO thankful for my @UMichCSE PIs for letting me go out on this long limb. Also for the training and mentorship from @robertpdickson and @CarlKoschmann. I'm now full-time in the @KoschmannLab and fully hooked on @nanopore for rapid cancer Dx
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18 Jul 2022
14) If you work in this space DM me! I'm so excited about potential use cases and improvements that can be made in extraction, targeted amplification, library preparation, wet-lab automation, squiggle-space informatics, base-space informatics, and would love to collaborate!