Scientific instrument developer, focused on #lightsheet and other #microscopy at @ASIImaging. 🔬 Rarely here; find me at jsdaniel02.bsky.social
Joined December 2016
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12 Photos and videos
6 Dec 2024
Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer and @Bin_YANG_Optics. vimeo.com/801341925
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6 Dec 2024
DaXi is a SOLS design that simultaneously achieves sub-micron resolution (450nm laterally and 2um axially) and large FOV (volume of 3000 x 800 x 300 um with galvo, more with stage scanning). Light sheet gentleness and speed. Multi-view capability. nature.com/articles/s41592-0…
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4 Dec 2022
At #CellBio2022? ASI has a new microscope to announce in the talk at 11:15am by @briankhake in theater 1 in the exhibition area, and afterwards the curtain will be pulled back. Booth 2025.
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23 Feb 2022
Most high-NA air objective lenses are coverslip-corrected but sometimes you want to image in air, e.g. high-NA SOLS. We have glued coverslips onto objectives for this. To check how the gluing went we take image stack of diffraction-limited beads supported on a coverslip. 🧵
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23 Feb 2022
If you want to do this yourself the process we’ve come to is 1) cut AR-coated coverslip to size; 2) apply silicone glue around the edge of the objective tip; 3) place coverslip 4) level it by iteratively pushing the edge with a toothpick; 5) double-check with beads after curing.
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23 Feb 2022
Iterative leveling is guided by bead stacks: take stack of beads, inspect reliced images, poke again. It’s tedious but algorithmic once you determine which coma direction corresponds to which tilt direction. In our hands, once the coma is gone then other aberrations are gone too.
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15 Nov 2021
Seem an appropriate cue to announce a MM2 plugin "LightSheetManager" to provide an easy interface to all light sheet microscopes, be they custom-built or from commercial parts. CC @Bin_YANG_Optics @DougPShepherd @kschink @MarijnSiemons @Maurice_Y_Lee @mattersOfLight @openspim
11 Nov 2021
There is certainly a software need for this type of microscope. We will also be working on it at the Biohub. Maybe we should do a coordinated community project? @mattersOfLight @loicaroyer
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15 Nov 2021
Goal #3: FOSS community effort with contributions from many individuals and institutions. To avoid duplication of effort the plugin should work with many microscopes in many contexts. Contributing to the project should be easy.
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15 Nov 2021
Current status: working on API definition and initial code. If you are interested in joining with us at this stage please get in touch with me and/or @nico_stuurman. Hoping to make the repo public in the coming months. Started by ASI and the Micro-Manager team.
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26 Mar 2021
I will be giving 2 #FOM2021 company talks:
- Mon 16:40 CET "A high-NA objective lens that works in water, CLARITY, BABB, and more"
- Tues 17:10 CET "How to turn a widefield microscope into a rapid 2D imaging system"
Descriptions follow:
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26 Mar 2021
Monday 16:40-17:10 CET Room B
A simple design trick enables an objective lens to work in any medium. I will describe this trick and discuss our latest all-immersion design with 3.4mm WD and NA 1.05 in water, 1.15 in CLARITY, and 1.24 in BABB. Help us make this lens a reality!
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26 Mar 2021
Tuesday 17:10-17:40 CET Room B
Imaging large 2D areas is usually done by tiling images with step/settle stage motion. I present a method for rapid 2D imaging -- like a slide scanner -- using a standard widefield microscope, a constant-speed stage, and a synchronized galvo.
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14 Oct 2020
@asiimaging will proceed with a production run of #KingSnout objectives if we receive at least 4 orders for them in the next 3 months, and we'll give a discount on those orders. Details at asiimaging.com/frontpage-art…. Credit to inventors @amsikking and @AndrewGYork.
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14 Oct 2020
A beautiful demonstration of their utility for high-NA SOLS with large FOV by @Bin_YANG_Optics and @loicaroyer is at doi.org/10.1101/2020.09.22.3….
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5 Jun 2020
I'm "published" again, a brief semi-technical overview article about light sheet microscopy for the Biophotonics magazine. Perhaps a good resource for people who want a "lightweight" overview of LSFM/SPIM. Formatting is bit weird in the HTML version photonics.com/Articles/Light….
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5 Jun 2020
So far I've only spotted 2 minor things I would like to change, there are probably more. Sigh...
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5 Jun 2020
It was an interesting experience explaining things technically correct but with the least jargon possible, within a tight space constraint.
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