ALT Microscopy image showing the presence of a P2RY12 expressing macrophages in the yolk sac

ALT Graph showing an inferred trajectory of the early yolk sac bypassing the monocyte stage to directly produce macrophages via an accelerated pathway

ALT Graph showing distinct waves of stem cells in the yolk sac and the varied pools of blood and immune cells they make pre and post AGM.

ALT Graphs showing the inferred emergence of the first blood and immune stem cells from specialised endothelium in the human yolk sac, paralleled in a model iPSC system developed by @ClaraAlsinet et al.

ALT FDG overlaid with PAGA showing trajectory of HE transition to HSPC in YS scRNA-seq data (n=3; CS10, 11 and 14; k=2,262) (top) and iPSC-derived HSPC scRNA-seq data (n=7, k=437) (21) (bottom), with feature plots of key genes (IL33, ALDH1A1) involved in endothelial to hemogenic transition

ALT Circular plots showing relative absorption probabilities of lineage-state transition between primitive HSPCs in the YS pre-AGM (CS10-11) (left) and primitive and definitive HSPCs in the YS post-AGM (>CS14) (right). Colour indicates the HSPC population density as a z-scored kernel density estimation (KDE) score and the position of HSPC population densities indicate respective lineage priming probability between Macrophage, lymphoid (NK and B lineage), erythroid and MK terminal states.