About 4 minutes of action from our best new intensiometric bioluminescent calcium indicator in HeLa cells exposed to l-histamine. Sorry for no time stamp or scale bar π₯Έ Seeing ΞL/L of >30-fold in some cells π w/@LABrechaun, contributions from @_theglowup, biorXiv soonπ€
Happy #FluorescenceFriday! Check out this time-lapse movie showing the extrusion of larval epidermal cells during Drosophila development. Adherens junctions are visualized by E-cadherin-GFP.
@Dev_journal publication: doi.org/10.1242/dev.179606
What is going on with these zebrafish basal skin cells? I invite you to a π§΅ on wound healing, hydraulic fracking, and macropinocytosis, from subcellular to tissue-scale! All part of my favorite PhD project, now up on bioRxiv (1/17)
biorxiv.org/content/10.1101/β¦
ALT Confocal maximum intensity projection timelapse of zebrafish basal epidermal cells reacting to a wound. Cells express cytoplasmic mCherry. Dark gaps emerge between cells, and dark spots appear in the cytoplasm. The fissures between cells propagate outwards in a wave.
We present light-sheet microscopy combined with structured illumination.
This enables rapid volumetric imaging in a light-sheet format with doubled resolution.
This will need a few tweets to discuss the details... 1/n
biorxiv.org/content/10.1101/β¦
A great solution for targeted sequencing of Monkeypox is available and has been validated in the field in the past. Please reach out to me or our Infectious Disease team at Illumina for information on the panel.