Most people don't pay attention to acquisition order on #confocal#microscopes.
Frequently, nuclear stains like DAPI are the first channel.
Instead, let the labelling inform you.
For example:
Channel 1: Desmoglein 1
Channel 2: Plakophilin 2
Channel 33342: Hoechst 33342
Surely this'll convince sCMOS manufacturers to do something.
ALT Meme: Sad Wolverine left out of party
Top: two people kissing with text "(s)CMOS correction algorithms" and "most (s)CMOS cameras)
Bottom: a more zoomed out shot with a frowning Wolverine with text: "cameras with manufacturer dark current correction that can't be turned off"