Joined October 2019
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4 Mar 2024
Welcome to StainsFile by @STEMCELLTech! Here, you can enjoy easy and open access to all the protocols and supporting resources you need to help you fix, prepare, and stain specimens: stainsfile.com 👩🏽🔬💻🧫🔬
#cellstaining #histology #microscopy #microscopymonday
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StainsFile retweeted
Congratulations to Christine Holt, Kelsey Martin, Erin Schuman, and Oswald Steward, winners of the 2026 Kavli Prize in Neuroscience. Their discoveries revealed how neurons make proteins locally to support brain development, learning, and plasticity.
#KavliPrize
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Jun 12
A new detailed analysis of the #histopathology of the tongue in the A17/ mouse model opens new avenues for understanding the mechanisms of #dysphagia.
Check out the work from the Raz lab at @UniLeidenNews in @AJPathology: bit.ly/4uZIoWR
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Jun 10
🔬 Great embedding starts with the right tools and techniques.
From molds and tampers to biopsy bags and tissue orientation, every detail matters when avoiding common #histology pitfalls.
Get all the tips you'll need to ace your next embedding session: bit.ly/4f6g4O5
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Jun 8
🧠 Working with delicate neural #organoids? Proper cryogenic tissue processing is critical for preserving structure and viability.
Check out this protocol for the best way to fixate, cryoprotect, embed, snap freeze, and cryosection your samples: bit.ly/4nGVupJ
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Jun 5
The Huber lab at @UofR is helping labs become more efficient.
They looked at gomori methenamine silver stains and determined that the standard two levels of #staining are not necessary in the majority of positive cases.
📝 bit.ly/4nETdLv
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StainsFile retweeted
New this month in JHC: "Two-step Gelatin Embedding Preserves Fibrocartilage Morphology, Enthesis, and Cartilage Stain of Bone Joints."
Click to read: journals.sagepub.com/doi/abs…
#cartilage #histology #immunostaining
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A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution
@michalj @ScienceMagazine 2024
science.org/doi/10.1126/scie…
H01 - 3D serial section scanning electron microscope data (4 nm resolution) of 1.05 mm3 human brain (left anterior middle temporal gyrus, epileptic)
h01-dot-neuroglancer-demo.ap…
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Jun 3
CryoPRISM is a new tool from researchers at @MIT for observing cellular machinery in a natural environment.
Dr. Joseph Davis, the study's lead, says that cryoPRISM is the sweet spot between preserving native structures and high resolution: bit.ly/3Peojx2
#Microscopy
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StainsFile retweeted
Bread and butter post: Inflammatory cloacogenic polyp is a mucosal prolapse polyp at the junction of rectal glandular mucosa and anal squamous mucosa. Note that the the lamina propria is filled with smooth muscle rather than the usual complement of inflammatory cells.
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Jun 1
Before tissues can be stained and studied, they need to be preserved and that’s where fixatives come in.
Selecting the appropriate fixative is an essential step in the staining process to ensure the foundation of your microscopy session is solid. Check out this database of fixatives to make sure you always choose right: bit.ly/3PB0TBS
#HistologyResource
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StainsFile retweeted
Take a look at these fluorescent egg chambers from the ovary of a fruit fly. Isn't the geometric nature of biology incredible?! #FluorescenceFriday
bit.ly/4v80ia8
Credit: Dr. Yujun Chen
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May 29
MRI quality is often hampered by noise.
Researchers from @Tsinghua_Uni have proposed a new iterative residual learning strategy entitled “Noise2Average” for denoising #MRI data with multiple repetitions.
Read the abstract: bit.ly/4ceuhXo
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StainsFile retweeted
Mechanical forces & cell shape guide how plant stomata form🥬👄
@RobinsonSci's team including @LeoSerra13 & Euan Smithers uncover how the interplay between cell shape & mechanical stress influences the orientation of stomata
doi.org/10.1016/j.celrep.202…
slcu.cam.ac.uk/news/mechanic…
ALT Confocal microscopy image of adaxial side of PM-YFP cotyledon showing new stomatal divisions. Newly formed stomata are highlighted in magenta at 2 days after germination. Imaging by Leo Serra.
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May 27
A new X-ray imaging technique could transform how hospitals analyse tissue samples, potentially speeding up diagnoses and improving outcomes for patients.
The study was led by researchers at @ucl: bit.ly/3QFpHc2
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StainsFile retweeted
New article in JHC: "Transforming Growth Factor-β Drives Epithelial Mesenchymal Transition and Reduces Synthesis of Unelaborated O-GalNAc Glycans in Breast Cancer Cells."
Click to read for FREE: journals.sagepub.com/doi/ful…
#breastcancer #EMT #microscopy
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High Grade Squamous Intraepithelial Lesion (HSIL) #TheJPCNCR #PathTwitter
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May 25
#Fixation sets the stage for everything that follows. 🔬
From preserving tissue structure to influencing staining outcomes, the choice of fixative matters more than you think.
Brush up on the fundamentals and make more informed decisions at the bench: bit.ly/4mF5IXa
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May 22
Researchers at @embl have tracked in real time nascent RNA structure formation, functionally characterizing up to 8 different types of RNA molecules.
Learn how they accomplished this with multicolor single-molecule #FluorescenceMicroscopy experiments: bit.ly/4mxAa5d
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StainsFile retweeted
What is a brain cell but not a neuron? It sounds like a riddle, but our brains are full of lesser-known cells besides the more well known neurons.
In this microscopy image, we see one such cell known as an astrocyte, named for their star-like shape. #SciShots
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StainsFile retweeted
Should scientists connect more directly with policymakers? 🏛️
The latest Lab Coats & Life™ Podcast explores how researchers can engage in policy, communicate clearly, and bring a human perspective to real-world decisions.
🎧 Listen wherever you get your podcasts.
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