(he/him) Light Microscopist. Tweets about microscopy 🔬, cell biology, and optics. No new posts here, but find me on bluesky: @tanner-fadero.bsky.social
Joined August 2017
- Tweets 2,241
- Following 1,453
- Followers 1,491
- Likes 5,575
548 Photos and videos
Pinned Tweet
24 Aug 2023
We are excited to announce the release of @manager_micro v2.0.2_20230815, which includes a new feature in the “On-The-Fly Processor Pipeline” menu: Deskew! Broadly, this is useful for automatically deskewing, rotating, and projecting light sheet images in Micro-Manager. (1/7)
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Jan 12
Magnetically-controllable antibodies!!!!!
🧲💉
Jan 12
Some exciting news to share — we've made the world's first magnetically controlled antibody! What is a magnetically controlled antibody? It's an antibody drug that you can turn on and off, wherever you want in the body. (1/9) x.com/ashleevance/status/201…
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8 Mar 2025
I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!
8 Mar 2025
First post.
NIH and medical research are under attack by the Trump admin. Cancer cures are being stopped.
Please follow us for more info.
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4 Feb 2025
Alright, I'm calling it. I'm over to the other place (rhymes with clue guy).
Find me at my new profile: tanner dash fadero at bee ess kay why dot social dot com.
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Tanner Fadero retweeted
2 Dec 2024
We present long-term stabilization of an oblique plane microscope (OPM).
By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift.
The feedback signal comes from a laser, no fluorescence is expended.
biorxiv.org/content/10.1101/…
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Tanner Fadero retweeted
9 Nov 2024
Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snouty microscope! They have a great team there with @nico_stuurman, @KariHerrington and @micaelalasser (Instagram). A great location!😀
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Tanner Fadero retweeted
4 Nov 2024
PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.
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31 Oct 2024
And thanks to @amsikking for running a thermal calibration on this exact King Snout lens!
18 Oct 2024
Here I measure the axial shift of the focal plane of an AMS-AGY v2 objective (a.k.a KingSnout) at ~2.0um/K, with a CLTE of ~19e-6/K (similar to brass):
github.com/amsikking/AMS_AGY…
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Tanner Fadero retweeted
28 Oct 2024
Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all:
beryl.mrc-lmb.cam.ac.uk/rlgc…
Feedback always welcome.
x.com/JamesDManton/status/18…
12 Aug 2024
We (@AndrewGYork, @RunningPhoton, @microRussell and I) are still working on the manuscript describing our automatic parameter-free deconvolution approach, but we've made a Google Colab notebook where you can try it out without needing to install anything:
beryl.mrc-lmb.cam.ac.uk/rlgc…
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23 Oct 2024
Made a prototype light trap out of aluminum shim stock and Musou black paint. Watching paint dry has never been so interesting. The matte finish is fantastic!
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Tanner Fadero retweeted
11 Oct 2024
Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, @JennySachweh, Bernhard Hampoelz and others uncover that the surrounding cellular milieu determines NPC composition via the nuclear transport system itself. #teamtomo #NPC A 🧵
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Tanner Fadero retweeted
10 Oct 2024
Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a collimated ~1mm⌀ 532nm laser aligned into the back of Nikon 40x0.95 air objective. Radially symmetric/concentric interference rings indicate good alignment.
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2 Oct 2024
Does anyone know what the effective focal length (EFL) and/or the mounting threads of @NikonInst's standard LWD 0.52 NA condenser lens is? Pic for context:
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4 Oct 2024
Here are the specs for anyone interested:
Mounting Threads: M48 x 0.75
Effective Focal Length: 28 mm
Working distance: 29.3 mm
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3 Oct 2024
Gentle Rhodamines for Live-Cell Fluorescence Microscopy | ACS Central Science pubs.acs.org/doi/10.1021/acs…
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2 Oct 2024
Woah cool! I'm going to try this O2/O3 mount for my snoutscope build at the @UCSF CALM
2 Oct 2024
This corresponds closely with the O2-O3 'cross brace' solution from @JLazzariDean. Based on the above data, reversing one of the POLARIS-K2T mounts will neutralize their thermal effect, leaving just the aluminum cross brace and an ~exact cancelation:
x.com/JLazzariDean/status/15…
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