The molecular interplay between viruses and cell plasma membrane regulating virus assembly. Viruses, Cell Membranes, Dynamics, Biophysics and Microscopy.
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ALT Screenshot of the linked article with the text: PERSPECTIVE| 28 OCTOBER 2024 Back to the future – 20 years of progress and developments in photonic microscopy and biological imaging Icon for The Forest of Biologists In collection: Imaging Marie Erard ORCID logo, Cyril Favard ORCID logo, Luke D. Lavis ORCID logo, Gaëlle Recher ORCID logo, Hervé Rigneault ORCID logo, Daniel Sage ORCID logo Author and article information J Cell Sci (2024) 137 (20): jcs262344. https://doi.org/10.1242/jcs.262344
ALT Hypothetical schematic representation of how actin-associated host cell factors that sense and promote membrane curvature – including the I-BAR domain-containing protein IRSp53, the Arp2/3-inhibitory cofactor Arpin and the ESCRT scission machinery – might facilitate HIV-1 assembly.
ALT Types of measurements for model development. (A) Analysis pipeline for extracting intensity and shape features from raw data. Step 1 is to segment the object of interest, which ranges from subcellular components to cellular structures and tissues. Step 2 is to track filaments, nuclei or cells either in real time via live-cell imaging or via pseudotime, which is the ordering of dynamic structures according to their progression in time (e.g. used to retrieve temporal data from time-course experiments using fixed samples). Step 3 is to extract features such as fluorescence intensity, shape or neighbourhood information from segmented objects. Finally, this will result in feature trajectories as a function of either absolute time, cell number or pseudotime. (B) Examples are shown of how to measure different types of tensions via pipette aspiration, laser ablation, optical tweezers or traction force microscopy.
ALT https://imabio-cnrs.fr/wp-content/uploads/2024/07/Programme_26062024.pdf?x49656