Mighty pleased with this one. My first paper from my postdoc at the Malhotra lab @CRGenomica. We challenge how 'bulky' cargo are organised in the ER lumen and propose a new model for their export. Big thanks to the good people at @JCellBiol for their handling of the paper!

Excited to see out in @Nature my postdoc work! “Membrane prewetting by #condensates promotes tight junction belt formation” How do #tightjunctions form a belt in #epithelia sealing our body cavities? @Honigmann_Lab @mpicbg @BIOTEC_TUD w/ #Jülicher @mpi_pks doi.org/10.1038/s41586-024-0…

Great Viewpoint from Vivek Malhotra @JCellBiol! COPII proteins, essential for cargo export from the ER, form not only coated small vesicles but also collars at tubule necks and phase-separate into liquid-like condensates, adapting to physiological demand. rupress.org/jcb/article/223/…

1/n My postdoc work on the evolution of mitosis in Ichthyosporea @Dey_Gautam @dudin_o @SchwabYannick groups is out @Nature shorturl.at/KPRQw Art by @nirupa_rao contrast fungal-like closed with animal-like open mitosis. Why do cells carry out mitosis in different ways?

#FluorescenceFriday #cellbiology #bioart Three examples of mitochondrial remodeling by lysosomes/late endosomes actively transported along microtubules, as seen by grazing incidence structured illumination microscopy at 1-2 frames/sec. doi.org/10.1016/j.cell.2018.…

Happy to share this mini-review that @TPucadyil and I wrote for Biochemical Society Transactions. portlandpress.com/biochemsoc…

🙌🏿 Congratulations to Vivek Malhotra on his election to the American Academy of Arts and Sciences @americanacad Dr Malhotra, group leader of the Intracellular Compartmentation lab at #CRG, joins a distinguished collection of leaders and professionals across many fields.

Our paper 'Analyzing the use of metaphors in news editorials for political framing' got accepted at the @naacl mains! Congratulations to all the co-authors @roxanneelbaff @miladalsh and Henning Wachsmuth. @AIHannover @trr_318 @webis_de #NLP #NLProc. See you in Mexico!

Our biophysical model in @PNASNews! ER export of collagen driven by a mechanochemical ratchet formed by expansion-contraction of intrinsically disordered regions (IDRs) of TANGO1, and gradients of pH/HSP47. IDR “breathing” produces work with each breath. pnas.org/doi/10.1073/pnas.23…

How can lysosomes, when ruptured, maintain stability allowing for repair? Glad to share @Nature nature.com/articles/s41586-0… the function of stress granules (SG) as molecular plugs that stabilise damaged membranes @TheCrick @maxgabgut #condensates #lysosomes #TB👇Thread

Collagen transport through inter-compartmental tunnels! Our theoretical model, led by the Pincet group @LP_ENS_, for how molecular gradients between organelles drive cargo transport through a tunnel - even without a cargo receptor. biorxiv.org/content/10.1101/…

Happy to share with you, our piece on how the molecular membrane fission machine - dynamin is regulated by 'gears' in its membrane binding PH-domain. @TPucadyil and I curated this brief review for Current Opinion in Cell Biology. Now online: authors.elsevier.com/c/1hPJK…

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec. We explore 4 intracellular and intercellular applications of TransitID: - discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix) - mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress - discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery - analysis of proteins that traffic intercellularly bw tumor cells & macrophages Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor authors.elsevier.com/a/1hKLc…

New on BioRxiv: Adhesion energy controls lipid-binding mediated endocytosis. A group effort lead by @RaluucaG with contributions from our friends @taraskalab @DimovaLab @cmatthaeus2 and Thomas Weikl @MpiciPotsdam @FUBiochemistry @FU_Berlin biorxiv.org/content/10.1101/…

1/2 Our stressed-out PhD scholars deserve a long-overdue increase in their stipends. The revision was due in 2022 after an already delayed raise in 2019. How can they meet their daily needs & undertake research amid rising inflation? @IndiaDST indiatoday.in/education-toda…

Our work about superPLD is now published! In this paper, we describe how we can harness mammalian cell-based directed evolution to engineer a membrane editor (phospholipase D; PLD) with superior performance in mammalian cells. 1/n nature.com/articles/s41557-0…

A tour the force of #geneediting and #STED. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms!

From the ER to outside the cell in 5 minutes - interested in visualizing membrane trafficking in live animals? Check out new paper @Dev_Cell authors.elsevier.com/a/1gsqZ… Congrats to @CarolinaCamelo6, @RaphaelSchleut2, @theajacobs_, @domfoerster twitterless colleagues @WWU_Muenster