@easonyshen profile picture
Yi (Eason) SHEN 沈溢 @easonyshen

Scientist & Protein Engineer | Husband & Dad

Joined July 2012
  • Tweets 375
  • Following 667
  • Followers 598
18 Photos and videos
Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD@abhi_aggarwal1
4 Apr 2024
Thrilled to share #TGECO1, a new blue-shifted Ca2 indicator for imaging neural activity, freshly published in Neurophotonics! Large 2PX, great for multiplexing! doi.org/10.1117/1.NPh.11.2.0… @Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary

ALT T-GECO1 Ca2 indicator

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Yi (Eason) SHEN 沈溢 retweeted
Ahmed Abdelfattah@Abdelfattah_Lab
20 Dec 2023
We are recruiting for an open Postdoc position. Interested in developing molecular tools? Our research is at the intersection of neuroscience, biochemistry, and biophysics. Please spread the word. Reach out via email if interested! @BrownUNeuro @CarneyInstitute
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Yi (Eason) SHEN 沈溢 retweeted
Joachim Goedhart@joachimgoedhart
28 Oct 2023
Red fluorescent proteins engineered from green fluorescent proteins pnas.org/doi/abs/10.1073/pna…
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Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD@abhi_aggarwal1
18 Oct 2023
Thrilled to share a new teal Ca2 indicator! #TGECO is a blue-shifted biosensor with high SNR & enhanced 2P absorption, great for #multiplexing in vivo!🧵 See preprint below⬇️ biorxiv.org/content/10.1101/… @Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary
T-GECO1 indicator structure

ALT T-GECO1 indicator structure

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Yi (Eason) SHEN 沈溢 retweeted
Joachim Goedhart@joachimgoedhart
15 Sep 2023
Photoswitchable binders enable temporal dissection of endogenous protein function biorxiv.org/content/10.1101/…
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Yi (Eason) SHEN 沈溢 retweeted
Helen Farrants@HelenFarrants
20 Jul 2023
Our #chemigenetic #fluorescent calcium indicator WHaloCaMP is now out on #bioRxiv : biorxiv.org/content/10.1101/… WHaloCaMP can be used to report on neuronal activity in flies, zebrafish and mice. WHaloCaMP is great for #multiplexedimaging. A 🧵:
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Introducing LSSmApple, a long Stokes shift red fluorescent protein, enabling the construction of 2nd gen dual-FP Matryoshka sensors for high throughput quantitative analysis with minimal spectral bleed-thru. biorxiv.org/content/10.1101/…

A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes...

Genetically encoded sensors enable quantitative imaging of analytes in live cells. State-of-the-art sensors are commonly constructed by combining ligand-binding domains with one or more sensitized...

biorxiv.org
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It's been a great pleasure to work with @jejike1 @MayuriSadoine Tom Kleist @healthy_crop & others on this project! Stay tuned for more follow-ups. LSSmApple plasmid is available on @Addgene addgene.org/191259/
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Yi (Eason) SHEN 沈溢 retweeted
Andrew York@AndrewGYork
12 Jul 2023
We discovered a surprisingly easy way to control the brightness of fluorescent proteins via magnetic fields: andrewgyork.github.io/gfp_ma… This raises fascinating scientific questions about the mechanism, and tantalizing technological opportunities for bioengineering.
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Yi (Eason) SHEN 沈溢 retweeted
Prof. Michael Lin@MichaelLinLab
6 Jul 2023
Today in @naturemethods: our new positively tuned voltage indicator, ASAP4e, for extended electrical recordings in the brain. ASAP4e achieves greater responsivity (>200% per 100mV vs 50% for other fluorescent voltage sensors) and better photostability. nature.com/articles/s41592-0…

A positively tuned voltage indicator for extended electrical recordings in the brain

Nature Methods - The ASAP4 family of genetically encoded voltage indicators allows recording of action potentials and subthreshold activity with either one- or two-photon microscopy over extended...

nature.com
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Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD@abhi_aggarwal1
4 May 2023
iGluSnFR3 is published! Check it out⬇️ nature.com/articles/s41592-0… We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission. This is #iGluSnFR3 in action detecting minis w/ TTX. @PodgorskiLab @HHMIJanelia @AllenInstitute
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Yi (Eason) SHEN 沈溢 retweeted
Yusuke Nasu
@NasuLab_IBC
29 Dec 2022
The latest versions of genetically encoded L-lactate biosensors, LACCO series, are out in bioRxiv: a second-generation green fluorescent extracellular biosensor eLACCO2.1 and a red fluorescent intracellular biosensor R-iLACCO1. #lactate #biosensor biorxiv.org/content/10.1101/…

Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular...

l-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. To enable investigations of both the inter- and intra-cellular dynamics of l-Lactate, we develop a...

biorxiv.org
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Yi (Eason) SHEN 沈溢 retweeted
Ben Campbell@lysergicdesigns
7 Nov 2022
Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s41592-0…

Chemically stable fluorescent proteins for advanced microscopy

Nature Methods - The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging...

nature.com
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Yi (Eason) SHEN 沈溢 retweeted
PLOS Biology@PLOSBiology
7 Sep 2022
#Potassium ions are an essential electrolyte in all biological systems. @easonyshen @Campbell_FPs &co describe high-performance genetically encoded K sensors to enable in vivo measurement of [K ] across multiple model organisms #PLOSBiology plos.io/3BkdEa3
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Yi (Eason) SHEN 沈溢 retweeted
Yusuke Nasu
@NasuLab_IBC
1 Sep 2022
Are you interested in a red fluorescent genetically encoded biosensor for extracellular L-lactate? Please check out our preprint on R-eLACCO2! biorxiv.org/content/10.1101/… Thank you so much, everyone contributing to this project! @Campbell_FPs

A red fluorescent genetically encoded biosensor for extracellular L-lactate

L-Lactate, traditionally recognized as a waste product of metabolism, is now appreciated as a key intercellular energy currency in mammals. To enable investigations of intercellular shuttling of...

biorxiv.org
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Yi (Eason) SHEN 沈溢 retweeted
Alexey Chizhik@ChizhikAlexey
28 Aug 2022
Inspired by a fantastic sculpture recently made by @mtyka. #SciArt
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Clearing the lab fridges today and found these gems! Happy #fluorescencefriday
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Yi (Eason) SHEN 沈溢 retweeted
Yong Qian@YongQian6
24 Aug 2022
How to do “multicolor imaging” with a “single color channel” on an ordinary fluorescent microscope? Here we show that temporally multiplexed imaging (TMI), a new principle of imaging, can do this magic.
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Yi (Eason) SHEN 沈溢 retweeted
Yulong Li Lab@yulonglilab
29 Jun 2022
Dopamine signals in RED! New rGRAB-DA sensors provide much higher in vivo sensitivity and SNR, and enable multiplex imaging with green sensors. #GRAB_sensors
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Yi (Eason) SHEN 沈溢 retweeted
Yulong Li Lab@yulonglilab
28 Jun 2022
Greatly improved green GRAB-DA sensors enable detection of cortical dopamine dynamics in vivo!
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