ALT Transient expression of HopF2-GFP in spinach Sp75 at the four-leaf stage. (A) GFP fluorescence images. G1 and G2 were infiltrated with Agrobacterium strain GV3101 (pSoup-p19) and AGL1 harboring pCAMBIA2300-HopF2-GFP constructs to observe the GFP fluorescence signal at 53 h, 64 h, 72 h after injection (HAI). Scale bar = 50 μm. (B) Fluorescent intensity and transformation efficiency of HopF2-GFP in Sp75 leaves using Agrobacterium strain GV3101 (pSoup-p19) and AGL1 at 53HAI, 64 HAI, 72HAI. AU: arbitrary unit. The experiments were repeated three times with similar results. Data presented are mean values ± SD (n ≥ 6). Different letters represent significant differences determined by one-way ANOVA with Tukey’s post hoc test (P < 0.05)
ALT Diagram of the workflow of tsCUT&Tag in plants. Image 1, plant tissues (plant breeding); image 2, protoplast (extraction); image 3, GFP-tagged TF vector construction; image 4, GFP image showing transient expression; image 5, CUT&Tag in transfected live cells; image 6, representation of DNA library construction.
