New preprint: We have developed an integrated pipeline to i) image gastruloids in toto with dual-view 2-photon🔬, ii) quantitatify cell and tissue-scale patterns of gene expression and morphometric features, iii) interact with the data in @napari_imaging. biorxiv.org/content/10.1101/…
📢Our preprint list is now up on FocalPlane. This list is focussed on new research in bioimage analysis. Drop us a message if there is a preprint that we've missed!
focalplane.biologists.com/20…
A first step towards a basis for research on #StemCell based #EmbryoModelsrdcu.be/dSHtz@NatureCellBio
We hope that this will be useful in this new field where quality control and appropriate criteria are needed
Happy that I could contribute 🙂 Flipper-TR also works well with 2-photon excitation 👇. It will be interesting too see in different systems what the probe can tell, and on which time scales. In this context, fast FLIM will be useful. Our attempt here: biorxiv.org/content/10.1101/…
Super happy to share with you a detailed tutorial covering everything you need to know about membrane mechanosensitive Flipper probes (🐬) using fluorescence lifetime microscopy. Accessible here: rdcu.be/dSmET See 🧵 below
New preprint: We have developed an integrated pipeline to i) image gastruloids in toto with dual-view 2-photon🔬, ii) quantitatify cell and tissue-scale patterns of gene expression and morphometric features, iii) interact with the data in @napari_imaging. biorxiv.org/content/10.1101/…
Great to be back in Basel for #BaCell3D. I will present a poster (#216) tomorrow on in toto imaging of organoids, which we use to investigate patterning mechanisms in gastruloids. Looking forward to discuss.
Organic dyes can photoconvert, having unexpected effects on quantitative microscopy. Check out this lovely paper from the Carravilla and Eggeling labs on how photoblueing impacts confocal, STED, and FLIM experiments, and how exchangeable probes can help! nature.com/articles/s41592-0…
I’m so happy to share another story from my PhD with @ErcanLab and @preibischs, where we studied the gene regulation in the dosage compensation system of C. elegans using smFISH! Here are a few highlights…🧵 biorxiv.org/content/10.1101/…
🔬🌟Join us for the 3rd @centuri_ls Hackathon for Quantitative Biology in Marseille!💥Both wet and dry lab people are welcome! Dive into amazing projects and experience this year stunning location!🌍 Registration now open!🚀💻#HackBio#QuantBio
⬇️Don't miss out, sign up now!⬇️
Very happy that I could contribute to this work led by @PRecouvreux. It has always fascinated me how cells coordinate using morphogens. When I started to get interested in biology, C.elegans was the first organism that we imaged at a🔬. Here, both came together with in vivo FCS.
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We are very pleased to see our study on Wnt spreading in C. elegans embryos published in @CurrentBiologycell.com/current-biology/ful… .
The outcome of an exciting collaboration between Vincent Bertrand's team and our team. A short thread on our main results!