@KleinerLabRNA profile picture
KleinerLab @KleinerLabRNA

The Kleiner lab investigates the chemical biology of RNA modifications and their role in gene expression regulation. kleiner.princeton.edu

Joined September 2021
  • Tweets 155
  • Following 456
  • Followers 744
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KleinerLab retweeted
brangwynnelab@brangwynnelab
21 Apr 2025
What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity to find out - Biggest surprise was impact of encoded protein function. Fantastic work w/ @LindsayABecker @sofiquinodoz @david_a_knowles biorxiv.org/content/10.1101/…

Genome-wide mapping of mesoscale neuronal RNA organization and condensation

Subcellular RNA organization can affect critical cellular functions. However, our understanding of RNA microenvironments, particularly biomolecular condensates, remains limited, largely due to a lack...

biorxiv.org
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KleinerLab retweeted
The Muir Lab@MuirLab
3 Apr 2025
The final version of our protein transposition manuscript is now published in @ScienceMagazine science.org/doi/10.1126/scie… We’ve added a few new things since our preprint /1

Protein editing using a coordinated transposition reaction

Protein engineering through the ligation of polypeptide fragments has proven enormously powerful for studying biochemical processes. In general, this strategy necessitates a final protein-folding...

science.org
The Muir Lab@MuirLab
4 Jun 2024
Ever wanted to swap out an entire internal region of a folded protein for functional biochemical studies? Check out our preprint that has been 5 years in the making, in which we establish a protein editing platform via protein transposition, enabling the insertion of functional cargo or PTMs into internal folded protein regions. #proteinengineering #proteinediting biorxiv.org/content/10.1101/…
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KleinerLab retweeted
Josh Riback@SuperSciJew
28 Feb 2025
In lab's #FIRSTPREPRINT, we present methods to measure nanometer organization around & between specific proteins in condensates. We uncover unexpected heterogeneity for a liquid-like phase with local meshwork spanning 10-50nm, stemming from ribosome biogenesis in the nucleolus.
bioRxiv Biophysics@biorxiv_biophys
28 Feb 2025
Nanometer condensate organization in live cells derived from partitioning measurements biorxiv.org/content/10.1101/… #biorxiv_biophys
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We are looking for a postdoc candidate in RNA chemical biology, broadly defined. Interested applicants can email me at rkleiner@princeton.edu with their CV, research summary, and names of 3 references. kleiner.princeton.edu/
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KleinerLab retweeted
Princeton University
@Princeton
14 Sep 2024
Faculty & students from @EmmanuelCollege, @CSUSBNews and @_hamptonu spent the summer with @PrincetonChem as part of the Visiting Faculty Research Partnership, working on projects that form the core of their research at home but with special focus at Frick: bit.ly/4edp5B8
Faculty and undergrads from Emmanuel College, Cal State San Bernardino and Hampton College

ALT Faculty and undergrads from Emmanuel College, Cal State San Bernardino and Hampton College

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KleinerLab retweeted
Princeton Chemistry@PrincetonChem
31 Jul 2024
Congratulations to Qais Jaber, awarded a two-year @EMBO Postdoctoral Fellowship supporting excellent postdoc research. Qais will continue his work in the @KleinerLabRNA deciphering RNA modifications in pathogenic bacteria.
Full-color photo of Qais standing on the staircase in Princeton Chemistry's Frick Lab.

ALT Full-color photo of Qais standing on the staircase in Princeton Chemistry's Frick Lab.

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KleinerLab retweeted
Jay Schneekloth@jschneek
8 Jul 2024
An honor to announce our book has been published! Many thanks to Martin Pettersson, the team at Wiley, and all our amazing contributors for making this exciting project happen! onlinelibrary.wiley.com/doi/…
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KleinerLab retweeted
Princeton Chemistry@PrincetonChem
14 Jun 2024
We are just opening the summer Visiting Faculty Research Partnership season and are thrilled to be hosting, among others, the Graham Chakafana Lab of @_HamptonU. The lab will be doing malaria research hosted by the @KleinerLabRNA. This marks their 2nd summer @PrincetonChem!
Three individuals (Professor Chakafana in the back) working in the lab at Princeton Chemistry.

ALT Three individuals (Professor Chakafana in the back) working in the lab at Princeton Chemistry.
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I'm very excited to share the news of my promotion. Much of the credit goes to the trainees that drove the research forward, and the invaluable mentorship that I received along the way from Michael Hecht, @davidrliu, @KapoorLab_RU, my Princeton colleagues, and many others!
Princeton Chemistry@PrincetonChem
30 May 2024
We are so very pleased to announce that @PrincetonChem’s Ralph Kleiner has been promoted to Associate Professor with tenure. Congratulations Ralph and the @KleinerLabRNA! Story here: bit.ly/4bJ4NhZ
Color, head-and-shoulders image of Ralph Kleiner against a white backgrop.

ALT Color, head-and-shoulders image of Ralph Kleiner against a white backgrop.

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Here is a photo of me with Crystal Dai after her PhD hooding ceremony this week. She was one of the first graduate students in the group and led the development of our activity-based probing platform for RNA modifying enzymes.
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KleinerLab retweeted
Ahmed H. Badran@AhmedHBadran
16 May 2024
Ever wanted to incorporate ncAAs into your protein but found the efficiency to be too low? Check out our new preprint, where we turned a simple observation in protein translation to an effective quadruplet codon translation strategy!
bioRxiv Synthetic Biology@bioRxiv_synbio
16 May 2024
Efficient Genetic Code Expansion Without Host Genome Modifications ift.tt/YImKuBd #biorxiv_synbio
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KleinerLab retweeted
Biochemistry@BiochemistryACS
2 May 2024
"Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms." Enabling technologies from @KleinerLabRNA pubs.acs.org/doi/10.1021/acs… #tbt #ACSChemicalBiology #methods @PrincetonChem
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KleinerLab retweeted
Princeton Chemistry@PrincetonChem
25 Apr 2024
What a great Take Your Child to Work Day here @PrincetonChem with faculty, visitors, and kids enjoying crafts, lunch, and a campus-wide chem demo (thank you, @PrincetonPhys). Well done Sarah and Angie! #TYCTWD24
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KleinerLab retweeted
Michael Fischbach
@mfgrp
11 Apr 2024
How to pick a problem. Today in @CellCellPress. 1/53 cell.com/cell/fulltext/S0092…

Problem choice and decision trees in science and engineering

Scientists and engineers often spend days choosing a problem and years solving it. This imbalance limits impact. Here, we offer a framework for problem choice: prompts for ideation, guidelines for...

cell.com
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Excited to share the latest manuscript from my lab and our first in @ACSCentSci . We used 5-BrUrd-modified tRNAs as activity-based probes to study the mechanism and substrate specificity of a human dihydrouridine synthase enzyme. Congrats to Jingwei and Nathan! @PrincetonChem
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pubs.acs.org/doi/10.1021/acs…

Sequence- and Structure-Specific tRNA Dihydrouridylation by hDUS2

The post-transcriptional reduction of uridine to dihydrouridine (D) by dihydrouridine synthase (DUS) enzymes is among the most ubiquitous transformations in RNA biology. D is found at multiple sites...

pubs.acs.org
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KleinerLab retweeted
Biochemistry@BiochemistryACS
5 Dec 2023
New issue! 📣 Read up on detection of RNA-protein interactions, selenoneine biosynthesis, riboswitches, sirtuins, functional amyloids, and more! pubs.acs.org/toc/bichaw/62/2… #amyloid #RNA #biosynthesis
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KleinerLab retweeted
The Haw Yang Lab@haw_lab
4 Dec 2023
Please see the amazing work done by @_xun_sun_ , published in Nat. Chem. today! Huge thanks to all of our great collaborators, Prof. Dr. Henning Mootz, Prof. Jhih-Wei Chu, Prof. Nozomi Ando (@AndoLab), and Prof. Tamiki Komatsuzaki (@HokkaidoUnivPR)! nature.com/articles/s41557-0…

Subdomain dynamics enable chemical chain reactions in non-ribosomal peptide synthetases

Nature Chemistry - Many natural products are produced by non-ribosomal peptide synthetases in an assembly-line fashion. How these molecular machines orchestrate the biochemical sequences has...

nature.com
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KleinerLab retweeted
bryan dickinson@chembioBryan
30 Nov 2023
Would you be surprised to learn that acid chlorides are not only compatible with biological research, in water mind you, but are in fact, AMAZING? Read on to see the answer… 1/n
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Two new preprints from our lab on human tRNA dihydrouridine (D) modifications and DUS writer enzymes: biorxiv.org/content/10.1101/… biorxiv.org/content/10.1101/…

A minimal sequence motif drives selective tRNA dihydrouridylation by hDUS2

The post-transcriptional reduction of uridine to dihydrouridine (D) by dihydrouridine synthase (DUS) enzymes is among the most ubiquitous transformations in RNA biology. D is found at multiple sites...

biorxiv.org
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In the first, we use chemoproteomic and transcriptomic methods to study human DUS proteins and globally map tRNA D sites. We then show that DUS proteins control the levels of a subset of tRNAs and codon-specific translation in a cell type-specific manner.
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In the second, we elucidate molecular principles underlying how hDUS2, a DUS protein associated with human cancers, installs a specific D residue in the tRNA D loop.
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