Via #OPG_Optica: Speckle illumination temporal focusing two-photon excited fluorescence microscopy bit.ly/42leuAl #DiffractionLimit #Bioimaging @CNRS

🚨NEW EPISODE ALERT🚨 How Super-Resolution Microscopy Changed Brain & Cancer Research | Prof. Markus Sauer How do you see structures inside cells that are smaller than the wavelength of light? Why was the diffraction limit considered an unbreakable barrier for decades, and how did super-resolution microscopy change everything? In this episode, we explore the frontiers of biological imaging with Prof. Markus Sauer, a pioneer of modern super-resolution microscopy. Prof. Sauer developed direct STORM (dSTORM), a technique that enables visualization of molecular organization at the nanometer scale. He leads the super-resolution microscopy lab at the Biozentrum, University of Würzburg, where his team continues to push the boundaries of cellular imaging. We discuss how single-molecule localization microscopy works, what are the technical challenges that we had to overcome to see the nanometer molecular world, and how these methods are now central to research in neuroscience, immunology, and translational science. The conversation also explores expansion microscopy, quantitative imaging, and the challenges of interpreting increasingly detailed biological data. This episode takes a closer look at how improved imaging reshapes our understanding of cells, and why higher resolution often leads to deeper questions. In this episode, you’ll learn about: - Important barriers to brake in order to achieve super-resolution microscopy - The principles behind dSTORM and single-molecule localization - What super-resolution reveals about molecular organization in neurons and in the context of immunology - Expansion microscopy and its impact on modern cell biology Subscribe to our YouTube channel and follow us for exciting neuroscience content. 🔗Link to our social media accounts: linktr.ee/neurosciencebeyond 🔗Link to the video: youtu.be/8cXV9J5NJQQ #Neuroscience #SuperResolutionMicroscopy #dSTORM #Microscopy #CellBiology #DiffractionLimit #ExpansionMicroscopy #Neuroimaging #SciencePodcast #Biophysics

View this Spotlight Summary by Aongus McCarthy of Single-photon laser reflective tomography over 7 km bit.ly/4q1yYIK #SpatialResolution #DiffractionLimit @StudyatUSTC

For #SpotlightSunday see Spotlight Analysis of Silica microdisk-based plasmonic whispering-gallery modes for refractive index and temperature sensing bit.ly/4jvWOZv #DiffractionLimit #FiberOpticSensors @NJU1902

Via #OPG_OL: Point-spread-function engineering in MINFLUX: optimality of donut and half-moon excitation patterns bit.ly/3BGwCe5 #ImageResolution #DiffractionLimit @EPFL

#OES_highlight Supercritical metalens at h-line for high-resolution direct laser writing doi.org/10.29026/oes.2024.23… by Prof. #JinghuaTeng @astar_research #metalens #DirectLaserWriting #laser #supercritical #lens #DiffractionLimit #ultraviolet #metasurface

'Diffraction limit: What light can't show' :Explained by Sh Vasudevan Mukunth @1amnerd #Diffraction #Light -resolution of instrument using light cant improve beyond #DiffractionLimit ,New tech ,#Optical #Microscopy #SuperResolution & More info #Science #UPSC Source:TH

An Editors' Pick via #OPG_JOSA_A: Dressing the cusp: how paraxial sharp-edge diffraction theory solves a basic issue in catastrophe optics bit.ly/3AJzCpm #FresnelDiffraction #DiffractionLimit @UnivRoma3

Via #OPG_AO: Multi-lens component used for a LWIR field-integral gas spectral imager bit.ly/3WigXb0 #DetectorArrays #DiffractionLimit

For over a century, we cursed the inexorable blur of diffracted light. Then, one man stopped trying to control how light behaves. Armed with a donut-shaped laser beam, he instead commanded where it shines and untethered resolution forever. This is how he did it: abberior.rocks/knowledge-bas… #StefanHell #STED #superresolution #diffractionlimit #abberiorKnowledgeBase

(2/2)Her lab is developing new approaches to bypass the #diffractionlimit and improve #singlemoleculelocalization. Current developments aimed at providing nanoscale structural and functional information in living cells, with the support @ERC_Research funding.

Via #OPG_AO: Polarization-controlled high-efficiency meta-couplers for coupling free-space light into subwavelength on-chip terahertz plasmonic devices ow.ly/5sqj50Sp8fh #DiffractionLimit #SurfacePlasmons @TJU1895

🌟Breaking the Limit! 🌟 A big shoutout to @AjasjaLjubetic lab for achieving the remarkable feat of breaking the #diffractionlimit on a #CTrap Edge system! 🔬 Using #DNApaint, they've resolved details as small as 20 nm - now that's ground-breaking! 🎉 Get ready for more incredible discoveries ahead!

Optical data #storage breakthrough increases capacity of diamonds by circumventing the #diffractionLimit @NatureNano doi.org/gs7w65 phys.org/news/2023-12-optica…

#TitleStory A Tunable Resolution Grating Monochromator and the Quest for Transform Limited Pulses By Josep Nicolas and Daniele Cocco from @SLAClab @advlightsource 👉 mdpi.com/2304-6732/9/6/367 #photonics #optics #freeelectronlaser #diffractionlimit

Virtual Superlensing Made a Reality spectrum.ieee.org/virtual-su… #TERAHERTZ #DIFFRACTIONLIMIT #SUPERLENS #MICROSCOPY #IMAGING

Via #OPG_Optica: Deeply subwavelength integrated excitonic van der Waals nanophotonics ow.ly/93Xl50PXLgI #DiffractionLimit @UCLAMechAeroEng

For over a century, we cursed the inexorable blur of diffracted light. Then, one man stopped trying to control how light behaves. Armed with a donut-shaped laser beam, he instead commanded where it shines and untethered resolution forever. This is how he did it: abberior.rocks/knowledge-bas… #StefanHell #STED #superresolution #diffractionlimit #abberiorKnowledgeBase

Via #OPG_Optica: High-fidelity ptychographic imaging of highly periodic structures enabled by vortex high harmonic beams ow.ly/8wSt50PPLfc #DiffractionLimit @STROBE_STC

Via #OPG_Optica: Passive superresolution imaging of incoherent objects ow.ly/55hP50PO14v #DiffractionLimit #ImageProcessing @OxfordPhysics