The Fluorescent Protein Database. tweets by @TalleyJLambert
Joined January 2019
- Tweets 129
- Following 1
- Followers 1,490
- Likes 121
10 Photos and videos
Try the new FPbase spectra viewer!
Completely rewritten to be faster, more customizable, and full-featured:
• SVG/PNG/CSV export
• state recovery & URL sharing
• keyboard shortcuts for rapid entry 💚
• efficiency calculations
• full documentation
fpbase.org/spectra
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Hi Fluorescence friends!
Please follow future updates on Bluesky:
bsky.app/profile/fpbase.org
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FPbase retweeted
13 Jun 2023
This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from @FPbase.
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FPbase retweeted
13 Dec 2022
Mutagenesis of #FbFP #LOV domain:
🟠 22 variants with 486-512 nm emission maxima
🟠 three-channel imaging via spectral unmixing
🟠 two-channel fluorescence lifetime unmixing
doi.org/10.1101/2022.12.09.5…
Congratulations, @ivangushchin, @Aynya5, and all the authors!
@FPbase
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FPbase retweeted
7 Nov 2022
Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s41592-0…
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Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing @mayankdu!
15 Sep 2022
Happy to share the Methods in Molecular Biology issue on Fluorescent Proteins 🌈🎉😀. Including many “much awaited” protocols on and about Fluorescent Proteins/applications. A big THANKS to authors across the globe for contributing. 👏🏻💐
link.springer.com/book/10.10…
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this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores
fpbase.org/microscope/u4gqNc…
cc @Daniel_Wa19
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FPbase retweeted
7 Dec 2021
🥁 Delighted to announce that our FPCountR paper is now on bioRxiv!
In this paper we describe the development of a method to allow absolute fluorescent protein quantification using microplate readers for synthetic circuit characterisation.
biorxiv.org/content/10.1101/…
🧵
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Always nice to see features put to use!
@eszter_cs and @gbstan use the FPbase API (among other things) in a method for the calibration of fluorescence readings on microplate readers; for quantification of FP concentration
biorxiv.org/content/10.1101/…
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FPbase retweeted
9 Oct 2021
Just discovered the interactive chart of FPbase.com. Wow, what an amazing tool to compare different properties across a wide range of fluorescent proteins. Great work @TalleyJLambert 🙂
fpbase.org/chart/
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FPbase retweeted
14 Oct 2021
FPbase is amazing! I created scope configurations for @UAZCancer core instruments, and link to it with a little QR code posted next to the scope! And made a nice lab exercise for my microscopy class. iScopeCalc rocks too. Keep ‘em coming, @TalleyJLambert!
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Writing a methods chapter on using FPbase for an upcoming MMB book... Will include things like
-Browsing/searching FPs on fpbase
-Spectra viewer
-microscope & reports feature
If there's an aspect or detail of FPbase that you wish were better documented, let me know!
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This is beautiful and makes me want to rewrite FPbase using it 😍
24 Aug 2021
Big project, first public release! 📢
✨ SQLModel ✨
...the biggest thing I've built since FastAPI & Typer. 🤓
SQL DBs based on Python type hints. 😎
Each model is both a Pydantic and @sqlalchemy model. 🤯
Optimized for FastAPI 🚀
github.com/tiangolo/sqlmodel
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Dear imaging companies,
Please tell your customers (or make available on request) the *actual* spectra for all components (LEDs, filters, etc) in their light path. just saying "460-490nm" is not sufficient.
It's amazing how many scientists can't get this info from their reps 😞
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a reminder on the difficulty in comparing fluorescence photobleaching measurements across studies (and even across probes on the same scope)
fpbase.org/bleaching/
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📣 Apply for an award from Addgene to attend a conference! We're accepting applications until April 30
☑️ Undergrad, grad student, postdoc, research tech
☑️ Working w/ fluorescent proteins
☑️ Planning to attend a conference, virtual, hybrid, or in-person
hubs.ly/H0LbWNd0
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Databases are hard... and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone!
Here's an example of what not to do if you find something needing correction 😂
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FPbase retweeted
4 Dec 2020
more JF spectra than you can shake a stick at. (2P coming soon)
thanks @rhodamine110!
fpbase.org/spectra/?s=6373,6…
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