Watching the inner life of a cell
Joined July 2019
- Tweets 23
- Following 3
- Followers 637
- Likes 5
2 Photos and videos
Bo Huang Lab retweeted
11 Nov 2025
What if pooled screens could measure dynamic cell behavior in real time?
RainBar uses fluorescent protein barcodes and cytoplasmic marker mediated segmentation to perform optical pooled screening in live cells.
Preprint: biorxiv.org/content/10.1101/…
ALT Highly accurate clustering of cells by RainBar barcode. Each cluster represents a unique fluorescent protein barcode identity used in optical pooled screening.
ALT Pooled cells expressing RainBar fluorescent barcodes imaged together in a single field during live cell imaging.
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11 Oct 2023
HybriSeq: our venture into single-cell RNA profiling by combing in situ hybridization and single-cell sequencing. doi.org/10.1101/2023.09.27.5…
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11 Oct 2023
CELL-E 2 is ready to transform protein sequences to images as well as generate sequences from images. bohuanglab.github.io/CELL-E_…
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31 May 2022
Here is our first step towards predicting cellular activities of proteins from their amino-acid sequences using deep-learning: biorxiv.org/content/10.1101/…
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11 Mar 2022
The OpenCell is now open! doi.org/10.1126/science.abi6…
11 Mar 2022
A tribute to team science! @labs_mann @czbiohub @kchev @ADBxMPIB @preethiragh @loicaroyer @marcoyannic @mattersOfLight @JswLab @BoHuangLab @liilii_tweet @heracanaj @yeaarachelll many other authors. So much fun to work with you all.
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Bo Huang Lab retweeted
20 Jul 2021
LR-ExSIM and LR-ExSTORM reveal subcellular protein organization. @XiaoyuShi2, Qi Li and colleagues report the development of label-retention expansion microscopy (LR-ExM): bit.ly/3qR6vXX
@IanSeiple @BoHuangLab @jiapeichen @dhivkmr @ReiterLab @ExMicroscopy
#Technology
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11 Jul 2021
LR-ExM and eSPIM also helped resolving endosome structures and dynamics in zebrafish embryo.
19 Jun 2021
We are so happy to help Su Guo lab at UCSF use our new method LR-ExM to visualize Par-3 assisted polarized endosome dynamics in zebrafish. pharm.ucsf.edu/guo, @BoHuangLab, @IanSeiple . advances.sciencemag.org/cont…
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11 Jul 2021
Thanks to @ReiterLab's cool work. LR-ExM is already helping discoveries in centrosome/ciliary structures.
9 Jul 2021
Dhivya Kumar @dhivkmr, @ReiterLab and colleagues identify a multi-protein complex called DISCO (DIStal centriole COmplex) that is essential for the initiation of cilium assembly. bit.ly/3dYZ3Vs
#cilia #centriole #centrosome #cellbiology #biochemistry #cytoskeleton
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6 Jul 2021
Expansion microscopy gets help fron new anchors
6 Jul 2021
.@XiaoyuShi2, Li, @IanSeiple @BoHuangLab et al. report the development of label-retention expansion #microscopy w/ a set of trifunctional anchors that not only prevent fluorescent signal loss but also enable high-efficiency labeling using SNAP & CLIP tags. bit.ly/3qR6vXX
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1 Jul 2021
Tagging and purification of endogenous proteins for single particle cryo-EM. Nice collaboration with @yifan_ucsf ! biorxiv.org/content/10.1101/…
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12 Apr 2021
Just coming out in @CellCellPress: authors.elsevier.com/sd/arti…. Our incredible collaboration with the
@BivonaLabUCSF and @TulpuleLab shows how cytoplasmic protein granules formed by a variety of oncogenic kinase fusion mutants can activate RAS/MAPK signaling.
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Bo Huang Lab retweeted
30 Mar 2021
Endogenous tagging 3D live-cell imaging mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB
30 Mar 2021
OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization biorxiv.org/cgi/content/shor… #bioRxiv
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28 Feb 2021
Transfer learning solves the challenge of training data collection for supervised learning and also improves the performance of self-supervised learning. For image denoising, see bioRxiv dx.doi.org/10.1101/2021.02.0… and GitHub github.com/BoHuangLab/Transf…
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2 Dec 2020
Here is a truly versatile and modular system for split enzymes, as demonstrated for split HaloTag: disq.us/t/3t8m5zq
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29 Sep 2020
Still more to be done, but here is the 3rd-gen of green-colored split fluorescent protein for protein tagging and signal amplification biorxiv.org/cgi/content/shor…
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Bo Huang Lab retweeted
14 Nov 2019
It takes a village: great collaboration between @czbiohub @JswLab @BoHuangLab Barbara Panning @LiGanLab - thanks to all.
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17 Sep 2019
The bright gets brighter: Split red fluorescent proteins for the visualization of endogenous proteins and synapses disq.us/t/3ibmj76
16 Jul 2019
Cytoplasmic protein granules organize kinase-mediated RAS signaling in cancer - our collaboration with Trever Bivona lab: biorxiv.org/content/10.1101/…
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