Very proud of the students and postdocs in my lab behind our new paper. We describe a set of rules to optimize germline expression and reagents to improve gene editing efficiency in C. elegans. nature.com/articles/s41467-0…
Excited that a talented Ph.D. student Mohammed Aljohani @MDJEditing from my lab is presenting his work on a genome-wide C. elegans CRISPR library for DNA and RNP-based CRISPR editing for the first time today at the CSHL CRISPR meeting.
Modular safe-harbor Transgene Insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. biorxiv.org/cgi/content/shor…#bioRxiv
Happy to see our paper on piRNA-mediated silencing and transgenerational inheritance in C.elegans in print.
Thanks to Sam Gu @ Rutgers for a great collaboration! nature.com/articles/s41592-0…
1/5 Our paper is out as a bioRxiv preprint. @sonia_elmouridi and @FaisalMAlkhaldi Like MosSCI, just better. Targeted single-copy and array integration synthesizing new and gradual improvements from the past 15 years across many labs. biorxiv.org/content/early/20…
I made an opto cell line and it's the coolest thing ever!
I feel like a wizard pointing at cells and making them contract 🧙♂️.
Here is local RhoA activation by recruitment of LARG.
That dorky sense of accomplishment when you finally find a restriction site for genotyping a knock-in that encodes a flexible linker dipeptide that does not introduce phosphorylatable amino acids. SfoI! In frame, encodes a gly-ala. AND cuts in Phusion HF buffer.
Looking for a better negative selection marker for C. elegans transgenesis than peel-1? My postdoc and Master's student developed a simple histamine-based system that works like a charm. pubmed.ncbi.nlm.nih.gov/3343…
Are genomes of identical twins really identical? "Our results indicate that allocations of cells during development shapes genomic differences between monozygotic twins." rdcu.be/cddS6@NatureGenet
I had to “sink or swim” in my undergrad lab, and because I swam, I assumed it was a good system. After having supportive mentors, I realize I could have learned so much more with proper guidance. Don’t let people sink just because it’s the way it was for you! #AcademicChatter
Our review (@timlu & Zhong lab) on designing living materials using synthetic biology is out! We covered recent advances & prospects and summarized #synbio tools for programming smart living materials with various functionalities.
rdcu.be/ccNg0nature.com/articles/s41578-0…
1/n Rounding out the best of 2020 for our lab, newly published “Engineering Cell Fate: Applying synthetic biology to cellular reprogramming.” We review cellular reprogramming #synbio Vision brought to life in vivid color by @nbwang22 and @beitz_adamsciencedirect.com/science/ar…
40 million years ago, ticks stole a gene from bacteria. Today, they use the enzyme that gene makes to fight off bacteria on our skin.
sciencenews.org/article/how-…